Model-based mutagenesis to improve the enantioselective fractionation properties of an antibody

Tarja Nevanen (Corresponding Author), Marja-Leena Hellman, Nana Munck, Gerd Wohlfahrt, Anu Koivula, Hans Söderlund

    Research output: Contribution to journalArticleScientificpeer-review

    18 Citations (Scopus)

    Abstract

    The binding affinity and specificity of recombinant antibodies can be modified by site‐directed mutagenesis. Here we have used molecular modelling of the variable domains of an enantiospecific antibody fragment to fine‐tune its affinity so it is more suitable for the fractionation of the drug enantiomers. We have shown earlier that the Fab fragment of this antibody specifically recognizes one enantiomer from the racemic mixture of a medical drug and that it can be used for the fractionation of these enantiomers by affinity chromatography. However, the affinity was unnecessarily high, requiring harsh elution conditions to release the bound enantiomer. Thus, the continuous use of the antibody affinity columns was impossible. We made a homology model of the antibody and designed mutations to the antigen‐binding site to decrease the affinity. Four out of five point mutations showed decreased affinity for the hapten. Two of the mutations were also combined to construct a double mutant. The affinity columns made using one of the single mutants with lowered affinity and the double mutant were capable of multiple rounds of enantioseparation.
    Original languageEnglish
    Pages (from-to)1089-1097
    Number of pages9
    JournalProtein Engineering
    Volume16
    Issue number12
    DOIs
    Publication statusPublished - 2003
    MoE publication typeA1 Journal article-refereed

    Keywords

    • antibodies
    • antibody Fab'-fragment
    • antibody fragments
    • mutagenesis
    • enantiomers
    • enantioselective antibody

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