Modification of hardwood dissolving pulp with purified Trichoderma reesei cellulases

Leena Rahkamo; Matti Siika-aho; Marianna Vehviläinen; Matti Dolk; Liisa Viikari; Pertti Nousiainen; Johanna Buchert

doi:10.1007/BF02228798

Modification of hardwood dissolving pulp with purified Trichoderma reesei cellulases

Leena Rahkamo^*, Matti Siika-aho, Marianna Vehviläinen, Matti Dolk, Liisa Viikari, Pertti Nousiainen, Johanna Buchert

^*Corresponding author for this work

Research output: Contribution to journal › Article › Scientific › peer-review

Abstract

Hardwood dissolving pulp was treated with purified Trichoderma reesei endoglucanases and cellobiohydrolases. Endoglucanases were more efficient in hydrolysing pulp carbohydrates than were the cellobiohydrolases at the same protein dosage. Endoglucanases also lowered the viscosity and improved the alkaline solubility more dramatically. There was a clear correlation between the alkaline solubility and viscosity, and therefore the solubility could only be improved by lowering the viscosity of the pulp. At the same degree of cellulose degradation, endoglucanase II was found to be most effective in reducing the viscosity and thus improving the solubility. Cellobiohydrolases had a less pronounced effect on the viscosity or solubility.

Original language	English
Pages (from-to)	153-163
Journal	Cellulose
Volume	3
Issue number	1
DOIs	https://doi.org/10.1007/BF02228798
Publication status	Published - 1996
MoE publication type	A1 Journal article-refereed

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title = "Modification of hardwood dissolving pulp with purified Trichoderma reesei cellulases",

abstract = "Hardwood dissolving pulp was treated with purified Trichoderma reesei endoglucanases and cellobiohydrolases. Endoglucanases were more efficient in hydrolysing pulp carbohydrates than were the cellobiohydrolases at the same protein dosage. Endoglucanases also lowered the viscosity and improved the alkaline solubility more dramatically. There was a clear correlation between the alkaline solubility and viscosity, and therefore the solubility could only be improved by lowering the viscosity of the pulp. At the same degree of cellulose degradation, endoglucanase II was found to be most effective in reducing the viscosity and thus improving the solubility. Cellobiohydrolases had a less pronounced effect on the viscosity or solubility.",

author = "Leena Rahkamo and Matti Siika-aho and Marianna Vehvil{\"a}inen and Matti Dolk and Liisa Viikari and Pertti Nousiainen and Johanna Buchert",

note = "Project code: B5SU00072",

year = "1996",

doi = "10.1007/BF02228798",

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T1 - Modification of hardwood dissolving pulp with purified Trichoderma reesei cellulases

AU - Rahkamo, Leena

AU - Siika-aho, Matti

AU - Vehviläinen, Marianna

AU - Dolk, Matti

AU - Viikari, Liisa

AU - Nousiainen, Pertti

AU - Buchert, Johanna

N1 - Project code: B5SU00072

PY - 1996

Y1 - 1996

N2 - Hardwood dissolving pulp was treated with purified Trichoderma reesei endoglucanases and cellobiohydrolases. Endoglucanases were more efficient in hydrolysing pulp carbohydrates than were the cellobiohydrolases at the same protein dosage. Endoglucanases also lowered the viscosity and improved the alkaline solubility more dramatically. There was a clear correlation between the alkaline solubility and viscosity, and therefore the solubility could only be improved by lowering the viscosity of the pulp. At the same degree of cellulose degradation, endoglucanase II was found to be most effective in reducing the viscosity and thus improving the solubility. Cellobiohydrolases had a less pronounced effect on the viscosity or solubility.

AB - Hardwood dissolving pulp was treated with purified Trichoderma reesei endoglucanases and cellobiohydrolases. Endoglucanases were more efficient in hydrolysing pulp carbohydrates than were the cellobiohydrolases at the same protein dosage. Endoglucanases also lowered the viscosity and improved the alkaline solubility more dramatically. There was a clear correlation between the alkaline solubility and viscosity, and therefore the solubility could only be improved by lowering the viscosity of the pulp. At the same degree of cellulose degradation, endoglucanase II was found to be most effective in reducing the viscosity and thus improving the solubility. Cellobiohydrolases had a less pronounced effect on the viscosity or solubility.

U2 - 10.1007/BF02228798

DO - 10.1007/BF02228798

M3 - Article

SN - 0969-0239

VL - 3

SP - 153

EP - 163

JO - Cellulose

JF - Cellulose

IS - 1

ER -

Modification of hardwood dissolving pulp with purified Trichoderma reesei cellulases

Abstract

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