Modification of hardwood dissolving pulp with purified Trichoderma reesei cellulases

Leena Rahkamo, Matti Siika-aho, Marianna Vehviläinen, Matti Dolk, Liisa Viikari, Pertti Nousiainen, Johanna Buchert

Research output: Contribution to journalArticleScientificpeer-review

87 Citations (Scopus)

Abstract

Hardwood dissolving pulp was treated with purified Trichoderma reesei endoglucanases and cellobiohydrolases. Endoglucanases were more efficient in hydrolysing pulp carbohydrates than were the cellobiohydrolases at the same protein dosage. Endoglucanases also lowered the viscosity and improved the alkaline solubility more dramatically. There was a clear correlation between the alkaline solubility and viscosity, and therefore the solubility could only be improved by lowering the viscosity of the pulp. At the same degree of cellulose degradation, endoglucanase II was found to be most effective in reducing the viscosity and thus improving the solubility. Cellobiohydrolases had a less pronounced effect on the viscosity or solubility.
Original languageEnglish
Pages (from-to)153-163
Number of pages11
JournalCellulose
Volume3
Issue number1
DOIs
Publication statusPublished - 1996
MoE publication typeA1 Journal article-refereed

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Cellulases
Hardwoods
Pulp
Cellulose 1,4-beta-Cellobiosidase
Solubility
Viscosity
Cellulase
Carbohydrates
Cellulose
Proteins
Degradation

Cite this

Rahkamo, Leena ; Siika-aho, Matti ; Vehviläinen, Marianna ; Dolk, Matti ; Viikari, Liisa ; Nousiainen, Pertti ; Buchert, Johanna. / Modification of hardwood dissolving pulp with purified Trichoderma reesei cellulases. In: Cellulose. 1996 ; Vol. 3, No. 1. pp. 153-163.
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abstract = "Hardwood dissolving pulp was treated with purified Trichoderma reesei endoglucanases and cellobiohydrolases. Endoglucanases were more efficient in hydrolysing pulp carbohydrates than were the cellobiohydrolases at the same protein dosage. Endoglucanases also lowered the viscosity and improved the alkaline solubility more dramatically. There was a clear correlation between the alkaline solubility and viscosity, and therefore the solubility could only be improved by lowering the viscosity of the pulp. At the same degree of cellulose degradation, endoglucanase II was found to be most effective in reducing the viscosity and thus improving the solubility. Cellobiohydrolases had a less pronounced effect on the viscosity or solubility.",
author = "Leena Rahkamo and Matti Siika-aho and Marianna Vehvil{\"a}inen and Matti Dolk and Liisa Viikari and Pertti Nousiainen and Johanna Buchert",
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Modification of hardwood dissolving pulp with purified Trichoderma reesei cellulases. / Rahkamo, Leena; Siika-aho, Matti; Vehviläinen, Marianna; Dolk, Matti; Viikari, Liisa; Nousiainen, Pertti; Buchert, Johanna.

In: Cellulose, Vol. 3, No. 1, 1996, p. 153-163.

Research output: Contribution to journalArticleScientificpeer-review

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AU - Rahkamo, Leena

AU - Siika-aho, Matti

AU - Vehviläinen, Marianna

AU - Dolk, Matti

AU - Viikari, Liisa

AU - Nousiainen, Pertti

AU - Buchert, Johanna

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AB - Hardwood dissolving pulp was treated with purified Trichoderma reesei endoglucanases and cellobiohydrolases. Endoglucanases were more efficient in hydrolysing pulp carbohydrates than were the cellobiohydrolases at the same protein dosage. Endoglucanases also lowered the viscosity and improved the alkaline solubility more dramatically. There was a clear correlation between the alkaline solubility and viscosity, and therefore the solubility could only be improved by lowering the viscosity of the pulp. At the same degree of cellulose degradation, endoglucanase II was found to be most effective in reducing the viscosity and thus improving the solubility. Cellobiohydrolases had a less pronounced effect on the viscosity or solubility.

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