Modification of spruce lignans with Trametes hirsuta laccase

Johanna Buchert, Annikka Mustranta, Tarja Tamminen, Peter Spetz, Bjarne Holmbom

Research output: Contribution to journalArticleScientificpeer-review

35 Citations (Scopus)

Abstract

The effect of Trametes hirsuta laccase on isolated spruce wood lignans was evaluated. Lignans were isolated from the heartwood of spruce branches and treated with different laccase dosages and treatment times. The effect of the treatment was monitored by gas chromatography, size exclusion chromatography and ionization difference UV spectroscopy. Lignans were efficiently oxidized by T. hirsuta laccase. About half of the phenolic groups present in lignans remained intact during the treatment. The oxidation of phenolic groups in lignans produced oligomeric structures containing approximately 4–5 lignan units (i.e., 8–10 phenyl propane units). Precipitation of the formed oligomeric structures probably prevented further polymerization.
Original languageEnglish
Pages (from-to)579-584
JournalHolzforschung
Volume56
Issue number6
DOIs
Publication statusPublished - 2002
MoE publication typeA1 Journal article-refereed

Fingerprint

Laccase
Lignans
Size exclusion chromatography
Ultraviolet spectroscopy
Propane
Gas chromatography
Ionization
Wood
Polymerization
Oxidation

Keywords

  • Laccase
  • Trametes hirsuta
  • Lignan
  • Hydroxymatairesinol
  • Spruce extractives

Cite this

Buchert, Johanna ; Mustranta, Annikka ; Tamminen, Tarja ; Spetz, Peter ; Holmbom, Bjarne. / Modification of spruce lignans with Trametes hirsuta laccase. In: Holzforschung. 2002 ; Vol. 56, No. 6. pp. 579-584.
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abstract = "The effect of Trametes hirsuta laccase on isolated spruce wood lignans was evaluated. Lignans were isolated from the heartwood of spruce branches and treated with different laccase dosages and treatment times. The effect of the treatment was monitored by gas chromatography, size exclusion chromatography and ionization difference UV spectroscopy. Lignans were efficiently oxidized by T. hirsuta laccase. About half of the phenolic groups present in lignans remained intact during the treatment. The oxidation of phenolic groups in lignans produced oligomeric structures containing approximately 4–5 lignan units (i.e., 8–10 phenyl propane units). Precipitation of the formed oligomeric structures probably prevented further polymerization.",
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Buchert, J, Mustranta, A, Tamminen, T, Spetz, P & Holmbom, B 2002, 'Modification of spruce lignans with Trametes hirsuta laccase', Holzforschung, vol. 56, no. 6, pp. 579-584. https://doi.org/10.1515/HF.2002.088

Modification of spruce lignans with Trametes hirsuta laccase. / Buchert, Johanna; Mustranta, Annikka; Tamminen, Tarja; Spetz, Peter; Holmbom, Bjarne.

In: Holzforschung, Vol. 56, No. 6, 2002, p. 579-584.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Modification of spruce lignans with Trametes hirsuta laccase

AU - Buchert, Johanna

AU - Mustranta, Annikka

AU - Tamminen, Tarja

AU - Spetz, Peter

AU - Holmbom, Bjarne

PY - 2002

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N2 - The effect of Trametes hirsuta laccase on isolated spruce wood lignans was evaluated. Lignans were isolated from the heartwood of spruce branches and treated with different laccase dosages and treatment times. The effect of the treatment was monitored by gas chromatography, size exclusion chromatography and ionization difference UV spectroscopy. Lignans were efficiently oxidized by T. hirsuta laccase. About half of the phenolic groups present in lignans remained intact during the treatment. The oxidation of phenolic groups in lignans produced oligomeric structures containing approximately 4–5 lignan units (i.e., 8–10 phenyl propane units). Precipitation of the formed oligomeric structures probably prevented further polymerization.

AB - The effect of Trametes hirsuta laccase on isolated spruce wood lignans was evaluated. Lignans were isolated from the heartwood of spruce branches and treated with different laccase dosages and treatment times. The effect of the treatment was monitored by gas chromatography, size exclusion chromatography and ionization difference UV spectroscopy. Lignans were efficiently oxidized by T. hirsuta laccase. About half of the phenolic groups present in lignans remained intact during the treatment. The oxidation of phenolic groups in lignans produced oligomeric structures containing approximately 4–5 lignan units (i.e., 8–10 phenyl propane units). Precipitation of the formed oligomeric structures probably prevented further polymerization.

KW - Laccase

KW - Trametes hirsuta

KW - Lignan

KW - Hydroxymatairesinol

KW - Spruce extractives

U2 - 10.1515/HF.2002.088

DO - 10.1515/HF.2002.088

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