Modification of spruce lignans with Trametes hirsuta laccase

Johanna Buchert, Annikka Mustranta, Tarja Tamminen, Peter Spetz, Bjarne Holmbom

Research output: Contribution to journalArticleScientificpeer-review

37 Citations (Scopus)


The effect of Trametes hirsuta laccase on isolated spruce wood lignans was evaluated. Lignans were isolated from the heartwood of spruce branches and treated with different laccase dosages and treatment times. The effect of the treatment was monitored by gas chromatography, size exclusion chromatography and ionization difference UV spectroscopy. Lignans were efficiently oxidized by T. hirsuta laccase. About half of the phenolic groups present in lignans remained intact during the treatment. The oxidation of phenolic groups in lignans produced oligomeric structures containing approximately 4–5 lignan units (i.e., 8–10 phenyl propane units). Precipitation of the formed oligomeric structures probably prevented further polymerization.
Original languageEnglish
Pages (from-to)579-584
Issue number6
Publication statusPublished - 2002
MoE publication typeA1 Journal article-refereed


  • Laccase
  • Trametes hirsuta
  • Lignan
  • Hydroxymatairesinol
  • Spruce extractives

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