Modular synthesis of self-assembling Janus-dendrimers and facile preparation of drug-loaded dendrimersomes

Sami Nummelin, Markus Selin, Sacha Legrand, Jarmo Ropponen, Jani Seitsonen, Antti Nykänen, Jari Koivisto, Jouni Hirvonen, Mauri A. Kostiainen, Luis M. Bimbo

Research output: Contribution to journalArticleScientificpeer-review

11 Citations (Scopus)

Abstract

Materials and methods aimed at the next generation of nanoscale carriers for drugs and other therapeutics are currently in great demand. Yet, creating these precise molecular arrangements in a feasible and straightforward manner represents a remarkable challenge. Herein we report a modular synthetic route for amphiphilic Janus-dendrimers via a copper-catalyzed click reaction (CuAAC) and a facile procedure, using simple injection, to obtain highly uniform dendrimersomes with efficient loading of the model drug compound propranolol. The resulting assemblies were analyzed by dynamic light scattering and cryogenic transmission electron microscopy revealing the formation of unilamellar and multilamellar dendrimersomes. The formation of a bilayer structure was confirmed using cryo-TEM and confocal microscopy visualization of an encapsulated solvatochromic dye (Nile Red). The dendrimersomes reported here are tunable in size, stable over time and display robust thermal stability in aqueous media. Our results expand the scope of dendrimer-based supramolecular colloidal systems and offer the means for one-step fabrication of drug-loaded dendrimersomes in the size range of 90-200 nm, ideal for biomedical applications.
Original languageEnglish
Pages (from-to)7189-7198
Number of pages10
JournalNanoscale
Volume9
Issue number21
DOIs
Publication statusPublished - 7 Jun 2017
MoE publication typeA1 Journal article-refereed

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Dendrimers
Transmission electron microscopy
Drug Carriers
Confocal microscopy
Dynamic light scattering
Propranolol
Pharmaceutical Preparations
Cryogenics
Copper
Thermodynamic stability
Coloring Agents
Visualization
Dyes
Display devices
Fabrication
nile red

Cite this

Nummelin, S., Selin, M., Legrand, S., Ropponen, J., Seitsonen, J., Nykänen, A., ... Bimbo, L. M. (2017). Modular synthesis of self-assembling Janus-dendrimers and facile preparation of drug-loaded dendrimersomes. Nanoscale, 9(21), 7189-7198. https://doi.org/10.1039/c6nr08102a
Nummelin, Sami ; Selin, Markus ; Legrand, Sacha ; Ropponen, Jarmo ; Seitsonen, Jani ; Nykänen, Antti ; Koivisto, Jari ; Hirvonen, Jouni ; Kostiainen, Mauri A. ; Bimbo, Luis M. / Modular synthesis of self-assembling Janus-dendrimers and facile preparation of drug-loaded dendrimersomes. In: Nanoscale. 2017 ; Vol. 9, No. 21. pp. 7189-7198.
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Nummelin, S, Selin, M, Legrand, S, Ropponen, J, Seitsonen, J, Nykänen, A, Koivisto, J, Hirvonen, J, Kostiainen, MA & Bimbo, LM 2017, 'Modular synthesis of self-assembling Janus-dendrimers and facile preparation of drug-loaded dendrimersomes', Nanoscale, vol. 9, no. 21, pp. 7189-7198. https://doi.org/10.1039/c6nr08102a

Modular synthesis of self-assembling Janus-dendrimers and facile preparation of drug-loaded dendrimersomes. / Nummelin, Sami; Selin, Markus; Legrand, Sacha; Ropponen, Jarmo; Seitsonen, Jani; Nykänen, Antti; Koivisto, Jari; Hirvonen, Jouni; Kostiainen, Mauri A.; Bimbo, Luis M.

In: Nanoscale, Vol. 9, No. 21, 07.06.2017, p. 7189-7198.

Research output: Contribution to journalArticleScientificpeer-review

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AU - Nummelin, Sami

AU - Selin, Markus

AU - Legrand, Sacha

AU - Ropponen, Jarmo

AU - Seitsonen, Jani

AU - Nykänen, Antti

AU - Koivisto, Jari

AU - Hirvonen, Jouni

AU - Kostiainen, Mauri A.

AU - Bimbo, Luis M.

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N2 - Materials and methods aimed at the next generation of nanoscale carriers for drugs and other therapeutics are currently in great demand. Yet, creating these precise molecular arrangements in a feasible and straightforward manner represents a remarkable challenge. Herein we report a modular synthetic route for amphiphilic Janus-dendrimers via a copper-catalyzed click reaction (CuAAC) and a facile procedure, using simple injection, to obtain highly uniform dendrimersomes with efficient loading of the model drug compound propranolol. The resulting assemblies were analyzed by dynamic light scattering and cryogenic transmission electron microscopy revealing the formation of unilamellar and multilamellar dendrimersomes. The formation of a bilayer structure was confirmed using cryo-TEM and confocal microscopy visualization of an encapsulated solvatochromic dye (Nile Red). The dendrimersomes reported here are tunable in size, stable over time and display robust thermal stability in aqueous media. Our results expand the scope of dendrimer-based supramolecular colloidal systems and offer the means for one-step fabrication of drug-loaded dendrimersomes in the size range of 90-200 nm, ideal for biomedical applications.

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