Modulating the Mechanical Performance of Macroscale Fibers through Shear-Induced Alignment and Assembly of Protein Nanofibrils

Ayaka Kamada, Aviad Levin, Zenon Toprakcioglu, Yi Shen, Viviane Lutz-Bueno, Kevin N. Baumann, Pezhman Mohammadi, Markus B. Linder, Raffaele Mezzenga, Tuomas P.J. Knowles (Corresponding Author)

Research output: Contribution to journalArticleScientificpeer-review

Abstract

Protein-based fibers are used by nature as high-performance materials in a wide range of applications, including providing structural support, creating thermal insulation, and generating underwater adhesives. Such fibers are commonly generated through a hierarchical self-assembly process, where the molecular building blocks are geometrically confined and aligned along the fiber axis to provide a high level of structural robustness. Here, this approach is mimicked by using a microfluidic spinning method to enable precise control over multiscale order during the assembly process of nanoscale protein nanofibrils into micro- and macroscale fibers. By varying the flow rates on chip, the degree of nanofibril alignment can be tuned, leading to an orientation index comparable to that of native silk. It is found that the Young's modulus of the resulting fibers increases with an increasing level of nanoscale alignment of the building blocks, suggesting that the mechanical properties of macroscopic fibers can be controlled through varying the level of ordering of the nanoscale building blocks. Capitalizing on strategies evolved by nature, the fabrication method allows for the controlled formation of macroscopic fibers and offers the potential to be applied for the generation of further novel bioinspired materials.
Original languageEnglish
Article number1904190
JournalSmall
DOIs
Publication statusE-pub ahead of print - 9 Oct 2019
MoE publication typeA1 Journal article-refereed

Fingerprint

Proteins
Silk
Microfluidics
Fibers
Elastic Modulus
Adhesives
Hot Temperature
Thermal insulation
Self assembly
Elastic moduli
Flow rate
Fabrication
Mechanical properties

Keywords

  • bioinspired materials
  • mechanical properties
  • microfluidic spinning
  • protein nanofibrils
  • structural orientation

Cite this

Kamada, A., Levin, A., Toprakcioglu, Z., Shen, Y., Lutz-Bueno, V., Baumann, K. N., ... Knowles, T. P. J. (2019). Modulating the Mechanical Performance of Macroscale Fibers through Shear-Induced Alignment and Assembly of Protein Nanofibrils. Small, [1904190]. https://doi.org/10.1002/smll.201904190
Kamada, Ayaka ; Levin, Aviad ; Toprakcioglu, Zenon ; Shen, Yi ; Lutz-Bueno, Viviane ; Baumann, Kevin N. ; Mohammadi, Pezhman ; Linder, Markus B. ; Mezzenga, Raffaele ; Knowles, Tuomas P.J. / Modulating the Mechanical Performance of Macroscale Fibers through Shear-Induced Alignment and Assembly of Protein Nanofibrils. In: Small. 2019.
@article{37c872d150214323b0fbd464bc6e4ccf,
title = "Modulating the Mechanical Performance of Macroscale Fibers through Shear-Induced Alignment and Assembly of Protein Nanofibrils",
abstract = "Protein-based fibers are used by nature as high-performance materials in a wide range of applications, including providing structural support, creating thermal insulation, and generating underwater adhesives. Such fibers are commonly generated through a hierarchical self-assembly process, where the molecular building blocks are geometrically confined and aligned along the fiber axis to provide a high level of structural robustness. Here, this approach is mimicked by using a microfluidic spinning method to enable precise control over multiscale order during the assembly process of nanoscale protein nanofibrils into micro- and macroscale fibers. By varying the flow rates on chip, the degree of nanofibril alignment can be tuned, leading to an orientation index comparable to that of native silk. It is found that the Young's modulus of the resulting fibers increases with an increasing level of nanoscale alignment of the building blocks, suggesting that the mechanical properties of macroscopic fibers can be controlled through varying the level of ordering of the nanoscale building blocks. Capitalizing on strategies evolved by nature, the fabrication method allows for the controlled formation of macroscopic fibers and offers the potential to be applied for the generation of further novel bioinspired materials.",
keywords = "bioinspired materials, mechanical properties, microfluidic spinning, protein nanofibrils, structural orientation",
author = "Ayaka Kamada and Aviad Levin and Zenon Toprakcioglu and Yi Shen and Viviane Lutz-Bueno and Baumann, {Kevin N.} and Pezhman Mohammadi and Linder, {Markus B.} and Raffaele Mezzenga and Knowles, {Tuomas P.J.}",
year = "2019",
month = "10",
day = "9",
doi = "10.1002/smll.201904190",
language = "English",
journal = "Small",
issn = "1613-6810",
publisher = "Wiley-VCH Verlag",

}

Kamada, A, Levin, A, Toprakcioglu, Z, Shen, Y, Lutz-Bueno, V, Baumann, KN, Mohammadi, P, Linder, MB, Mezzenga, R & Knowles, TPJ 2019, 'Modulating the Mechanical Performance of Macroscale Fibers through Shear-Induced Alignment and Assembly of Protein Nanofibrils', Small. https://doi.org/10.1002/smll.201904190

Modulating the Mechanical Performance of Macroscale Fibers through Shear-Induced Alignment and Assembly of Protein Nanofibrils. / Kamada, Ayaka; Levin, Aviad; Toprakcioglu, Zenon; Shen, Yi; Lutz-Bueno, Viviane; Baumann, Kevin N.; Mohammadi, Pezhman; Linder, Markus B.; Mezzenga, Raffaele; Knowles, Tuomas P.J. (Corresponding Author).

In: Small, 09.10.2019.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Modulating the Mechanical Performance of Macroscale Fibers through Shear-Induced Alignment and Assembly of Protein Nanofibrils

AU - Kamada, Ayaka

AU - Levin, Aviad

AU - Toprakcioglu, Zenon

AU - Shen, Yi

AU - Lutz-Bueno, Viviane

AU - Baumann, Kevin N.

AU - Mohammadi, Pezhman

AU - Linder, Markus B.

AU - Mezzenga, Raffaele

AU - Knowles, Tuomas P.J.

PY - 2019/10/9

Y1 - 2019/10/9

N2 - Protein-based fibers are used by nature as high-performance materials in a wide range of applications, including providing structural support, creating thermal insulation, and generating underwater adhesives. Such fibers are commonly generated through a hierarchical self-assembly process, where the molecular building blocks are geometrically confined and aligned along the fiber axis to provide a high level of structural robustness. Here, this approach is mimicked by using a microfluidic spinning method to enable precise control over multiscale order during the assembly process of nanoscale protein nanofibrils into micro- and macroscale fibers. By varying the flow rates on chip, the degree of nanofibril alignment can be tuned, leading to an orientation index comparable to that of native silk. It is found that the Young's modulus of the resulting fibers increases with an increasing level of nanoscale alignment of the building blocks, suggesting that the mechanical properties of macroscopic fibers can be controlled through varying the level of ordering of the nanoscale building blocks. Capitalizing on strategies evolved by nature, the fabrication method allows for the controlled formation of macroscopic fibers and offers the potential to be applied for the generation of further novel bioinspired materials.

AB - Protein-based fibers are used by nature as high-performance materials in a wide range of applications, including providing structural support, creating thermal insulation, and generating underwater adhesives. Such fibers are commonly generated through a hierarchical self-assembly process, where the molecular building blocks are geometrically confined and aligned along the fiber axis to provide a high level of structural robustness. Here, this approach is mimicked by using a microfluidic spinning method to enable precise control over multiscale order during the assembly process of nanoscale protein nanofibrils into micro- and macroscale fibers. By varying the flow rates on chip, the degree of nanofibril alignment can be tuned, leading to an orientation index comparable to that of native silk. It is found that the Young's modulus of the resulting fibers increases with an increasing level of nanoscale alignment of the building blocks, suggesting that the mechanical properties of macroscopic fibers can be controlled through varying the level of ordering of the nanoscale building blocks. Capitalizing on strategies evolved by nature, the fabrication method allows for the controlled formation of macroscopic fibers and offers the potential to be applied for the generation of further novel bioinspired materials.

KW - bioinspired materials

KW - mechanical properties

KW - microfluidic spinning

KW - protein nanofibrils

KW - structural orientation

UR - http://www.scopus.com/inward/record.url?scp=85073956492&partnerID=8YFLogxK

U2 - 10.1002/smll.201904190

DO - 10.1002/smll.201904190

M3 - Article

AN - SCOPUS:85073956492

JO - Small

JF - Small

SN - 1613-6810

M1 - 1904190

ER -