Modulation of skin collagen metabolism by irradiation

Collagen synthesis is increased in irradiated human skin

R. Riekki, A. Jukkola, M. L. Sassi, Matti Höyhtyä, M. Kallioinen, J. Risteli, A. Oikarinen (Corresponding Author)

Research output: Contribution to journalArticleScientificpeer-review

33 Citations (Scopus)

Abstract

Radiation‐induced fibrosis is a common side‐effect of cancer treatment. The pathophysiological events leading to fibrosis are not known in detail. We analysed the effect of therapeutic irradiation on human skin collagen synthesis, skin thickness, gelatinases and their inhibitors. Twenty randomly chosen women who had been treated for breast cancer with surgery and radiation therapy participated in the study. In each patient, the irradiated skin area was compared with a corresponding non‐treated skin area. Suction blister fluid (SBF) and serum samples were analysed for the aminoterminal propeptides of type I and type III procollagens (PINP and PIIINP), tissue inhibitors of matrix metalloproteinases (MMPs) 1 and 2 (TIMP‐1 and TIMP‐2) and MMP‐9 and MMP‐2/TIMP‐2 complex. Skin biopsies were analysed for PINP and immunohistochemical staining was used for PIIINP. In irradiated skin, PINP, PIIINP, TIMP‐1 and MMP‐2/TIMP‐2 complex levels in SBF and the number of PINP‐positive fibroblasts in tissue sections were significantly higher in comparison with non‐treated skin. The levels of TIMP‐2 in irradiated and non‐irradiated skin were similar. MMP‐9 could not be detected in SBF with the assay used. The serum levels of MMP‐9 were higher in the treated subjects than the reference values. The serum values of PINP, PIIINP, TIMP‐1, TIMP‐2 and MMP‐2/TIMP‐2 complex were not significantly affected. These results indicate increased local collagen synthesis and accumulation of connective tissue in irradiated skin. The marked upregulation of collagen synthesis as a result of irradiation offers a possibility to treat this complication with compounds such as topical steroids which downregulate collagen synthesis.
Original languageEnglish
Pages (from-to)874 - 880
Number of pages7
JournalBritish Journal of Dermatology
Volume142
Issue number5
DOIs
Publication statusPublished - 2000
MoE publication typeA1 Journal article-refereed

Fingerprint

Collagen
Skin
Suction
Blister
Fibrosis
Serum
Tissue Inhibitor of Metalloproteinase-2
Matrix Metalloproteinase 1
Tissue Inhibitor of Metalloproteinase-1
Collagen Type III
Matrix Metalloproteinase Inhibitors
Matrix Metalloproteinase 2
Therapeutic Uses
Connective Tissue
Reference Values
Up-Regulation
Radiotherapy
Down-Regulation
Fibroblasts
Steroids

Cite this

Riekki, R., Jukkola, A., Sassi, M. L., Höyhtyä, M., Kallioinen, M., Risteli, J., & Oikarinen, A. (2000). Modulation of skin collagen metabolism by irradiation: Collagen synthesis is increased in irradiated human skin. British Journal of Dermatology, 142(5), 874 - 880. https://doi.org/10.1046/j.1365-2133.2000.03465.x
Riekki, R. ; Jukkola, A. ; Sassi, M. L. ; Höyhtyä, Matti ; Kallioinen, M. ; Risteli, J. ; Oikarinen, A. / Modulation of skin collagen metabolism by irradiation : Collagen synthesis is increased in irradiated human skin. In: British Journal of Dermatology. 2000 ; Vol. 142, No. 5. pp. 874 - 880.
@article{d1fdd417c0ca463191d926571d89d5c6,
title = "Modulation of skin collagen metabolism by irradiation: Collagen synthesis is increased in irradiated human skin",
abstract = "Radiation‐induced fibrosis is a common side‐effect of cancer treatment. The pathophysiological events leading to fibrosis are not known in detail. We analysed the effect of therapeutic irradiation on human skin collagen synthesis, skin thickness, gelatinases and their inhibitors. Twenty randomly chosen women who had been treated for breast cancer with surgery and radiation therapy participated in the study. In each patient, the irradiated skin area was compared with a corresponding non‐treated skin area. Suction blister fluid (SBF) and serum samples were analysed for the aminoterminal propeptides of type I and type III procollagens (PINP and PIIINP), tissue inhibitors of matrix metalloproteinases (MMPs) 1 and 2 (TIMP‐1 and TIMP‐2) and MMP‐9 and MMP‐2/TIMP‐2 complex. Skin biopsies were analysed for PINP and immunohistochemical staining was used for PIIINP. In irradiated skin, PINP, PIIINP, TIMP‐1 and MMP‐2/TIMP‐2 complex levels in SBF and the number of PINP‐positive fibroblasts in tissue sections were significantly higher in comparison with non‐treated skin. The levels of TIMP‐2 in irradiated and non‐irradiated skin were similar. MMP‐9 could not be detected in SBF with the assay used. The serum levels of MMP‐9 were higher in the treated subjects than the reference values. The serum values of PINP, PIIINP, TIMP‐1, TIMP‐2 and MMP‐2/TIMP‐2 complex were not significantly affected. These results indicate increased local collagen synthesis and accumulation of connective tissue in irradiated skin. The marked upregulation of collagen synthesis as a result of irradiation offers a possibility to treat this complication with compounds such as topical steroids which downregulate collagen synthesis.",
author = "R. Riekki and A. Jukkola and Sassi, {M. L.} and Matti H{\"o}yhty{\"a} and M. Kallioinen and J. Risteli and A. Oikarinen",
year = "2000",
doi = "10.1046/j.1365-2133.2000.03465.x",
language = "English",
volume = "142",
pages = "874 -- 880",
journal = "British Journal of Dermatology",
issn = "0007-0963",
publisher = "Wiley-Blackwell",
number = "5",

}

Riekki, R, Jukkola, A, Sassi, ML, Höyhtyä, M, Kallioinen, M, Risteli, J & Oikarinen, A 2000, 'Modulation of skin collagen metabolism by irradiation: Collagen synthesis is increased in irradiated human skin', British Journal of Dermatology, vol. 142, no. 5, pp. 874 - 880. https://doi.org/10.1046/j.1365-2133.2000.03465.x

Modulation of skin collagen metabolism by irradiation : Collagen synthesis is increased in irradiated human skin. / Riekki, R.; Jukkola, A.; Sassi, M. L.; Höyhtyä, Matti; Kallioinen, M.; Risteli, J.; Oikarinen, A. (Corresponding Author).

In: British Journal of Dermatology, Vol. 142, No. 5, 2000, p. 874 - 880.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Modulation of skin collagen metabolism by irradiation

T2 - Collagen synthesis is increased in irradiated human skin

AU - Riekki, R.

AU - Jukkola, A.

AU - Sassi, M. L.

AU - Höyhtyä, Matti

AU - Kallioinen, M.

AU - Risteli, J.

AU - Oikarinen, A.

PY - 2000

Y1 - 2000

N2 - Radiation‐induced fibrosis is a common side‐effect of cancer treatment. The pathophysiological events leading to fibrosis are not known in detail. We analysed the effect of therapeutic irradiation on human skin collagen synthesis, skin thickness, gelatinases and their inhibitors. Twenty randomly chosen women who had been treated for breast cancer with surgery and radiation therapy participated in the study. In each patient, the irradiated skin area was compared with a corresponding non‐treated skin area. Suction blister fluid (SBF) and serum samples were analysed for the aminoterminal propeptides of type I and type III procollagens (PINP and PIIINP), tissue inhibitors of matrix metalloproteinases (MMPs) 1 and 2 (TIMP‐1 and TIMP‐2) and MMP‐9 and MMP‐2/TIMP‐2 complex. Skin biopsies were analysed for PINP and immunohistochemical staining was used for PIIINP. In irradiated skin, PINP, PIIINP, TIMP‐1 and MMP‐2/TIMP‐2 complex levels in SBF and the number of PINP‐positive fibroblasts in tissue sections were significantly higher in comparison with non‐treated skin. The levels of TIMP‐2 in irradiated and non‐irradiated skin were similar. MMP‐9 could not be detected in SBF with the assay used. The serum levels of MMP‐9 were higher in the treated subjects than the reference values. The serum values of PINP, PIIINP, TIMP‐1, TIMP‐2 and MMP‐2/TIMP‐2 complex were not significantly affected. These results indicate increased local collagen synthesis and accumulation of connective tissue in irradiated skin. The marked upregulation of collagen synthesis as a result of irradiation offers a possibility to treat this complication with compounds such as topical steroids which downregulate collagen synthesis.

AB - Radiation‐induced fibrosis is a common side‐effect of cancer treatment. The pathophysiological events leading to fibrosis are not known in detail. We analysed the effect of therapeutic irradiation on human skin collagen synthesis, skin thickness, gelatinases and their inhibitors. Twenty randomly chosen women who had been treated for breast cancer with surgery and radiation therapy participated in the study. In each patient, the irradiated skin area was compared with a corresponding non‐treated skin area. Suction blister fluid (SBF) and serum samples were analysed for the aminoterminal propeptides of type I and type III procollagens (PINP and PIIINP), tissue inhibitors of matrix metalloproteinases (MMPs) 1 and 2 (TIMP‐1 and TIMP‐2) and MMP‐9 and MMP‐2/TIMP‐2 complex. Skin biopsies were analysed for PINP and immunohistochemical staining was used for PIIINP. In irradiated skin, PINP, PIIINP, TIMP‐1 and MMP‐2/TIMP‐2 complex levels in SBF and the number of PINP‐positive fibroblasts in tissue sections were significantly higher in comparison with non‐treated skin. The levels of TIMP‐2 in irradiated and non‐irradiated skin were similar. MMP‐9 could not be detected in SBF with the assay used. The serum levels of MMP‐9 were higher in the treated subjects than the reference values. The serum values of PINP, PIIINP, TIMP‐1, TIMP‐2 and MMP‐2/TIMP‐2 complex were not significantly affected. These results indicate increased local collagen synthesis and accumulation of connective tissue in irradiated skin. The marked upregulation of collagen synthesis as a result of irradiation offers a possibility to treat this complication with compounds such as topical steroids which downregulate collagen synthesis.

U2 - 10.1046/j.1365-2133.2000.03465.x

DO - 10.1046/j.1365-2133.2000.03465.x

M3 - Article

VL - 142

SP - 874

EP - 880

JO - British Journal of Dermatology

JF - British Journal of Dermatology

SN - 0007-0963

IS - 5

ER -