Molecular analysis of a Phanerochaete chrysosporium lignin peroxidase gene

Isabelle Walther, M. Kälin, Jakob Reiser, F. Suter, B. Fritsche, Markku Saloheimo, Matti Leisola, Tuula Teeri, Jonathan Knowles, A. Fiechter

Research output: Contribution to journalArticleScientificpeer-review

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Abstract

The basidiomycete fungus Phanerochaete chrysosporium produces a number of extracellular peroxidases which appear to be important for lignin degradation.
We present here the isolation and complete nucleotide (nt) sequence of a gene (Ipo) coding for lignin peroxidase (LPO), the coding region of which is identical to a Ipo cDNA sequence which had previously been described [M. Tien and C.-P.D. Tu, Nature 326 (1987) 520–523]. The deduced amino acid (aa) sequence corresponds to 372 aa residues and the coding region is interrupted by eight short introns that range m size from 50 to 62 nt.
Southern blot experiments using the cloned Ipo gene as a hybridization probe revealed a complex restriction fragment pattern, indicating that there are a number of lpo-rElated nucleotide sequences present m P. chrysosporium DNA which cross-hybridize.
We also present data on the in vivo expression of the Ipo genes and show that they are regulated at the RNA level and that the structure of the transcripts as judged from Sl experiments is complex.
These data are consistent with the idea that there are a number of related Ipo genes in P. chrysosporium which constitute a gene family.
Original languageEnglish
Pages (from-to)127-137
JournalGene
Volume70
Issue number1
DOIs
Publication statusPublished - 1988
MoE publication typeA1 Journal article-refereed

Fingerprint

Phanerochaete
Genes
Peroxidases
Basidiomycota
Lignin
Southern Blotting
Introns
Amino Acid Sequence
Fungi
Nucleotides
Complementary DNA
RNA
Gene Expression
Amino Acids
lignin peroxidase
DNA

Cite this

Walther, I., Kälin, M., Reiser, J., Suter, F., Fritsche, B., Saloheimo, M., ... Fiechter, A. (1988). Molecular analysis of a Phanerochaete chrysosporium lignin peroxidase gene. Gene, 70(1), 127-137. https://doi.org/10.1016/0378-1119(88)90111-4
Walther, Isabelle ; Kälin, M. ; Reiser, Jakob ; Suter, F. ; Fritsche, B. ; Saloheimo, Markku ; Leisola, Matti ; Teeri, Tuula ; Knowles, Jonathan ; Fiechter, A. / Molecular analysis of a Phanerochaete chrysosporium lignin peroxidase gene. In: Gene. 1988 ; Vol. 70, No. 1. pp. 127-137.
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abstract = "The basidiomycete fungus Phanerochaete chrysosporium produces a number of extracellular peroxidases which appear to be important for lignin degradation. We present here the isolation and complete nucleotide (nt) sequence of a gene (Ipo) coding for lignin peroxidase (LPO), the coding region of which is identical to a Ipo cDNA sequence which had previously been described [M. Tien and C.-P.D. Tu, Nature 326 (1987) 520–523]. The deduced amino acid (aa) sequence corresponds to 372 aa residues and the coding region is interrupted by eight short introns that range m size from 50 to 62 nt. Southern blot experiments using the cloned Ipo gene as a hybridization probe revealed a complex restriction fragment pattern, indicating that there are a number of lpo-rElated nucleotide sequences present m P. chrysosporium DNA which cross-hybridize. We also present data on the in vivo expression of the Ipo genes and show that they are regulated at the RNA level and that the structure of the transcripts as judged from Sl experiments is complex. These data are consistent with the idea that there are a number of related Ipo genes in P. chrysosporium which constitute a gene family.",
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Walther, I, Kälin, M, Reiser, J, Suter, F, Fritsche, B, Saloheimo, M, Leisola, M, Teeri, T, Knowles, J & Fiechter, A 1988, 'Molecular analysis of a Phanerochaete chrysosporium lignin peroxidase gene', Gene, vol. 70, no. 1, pp. 127-137. https://doi.org/10.1016/0378-1119(88)90111-4

Molecular analysis of a Phanerochaete chrysosporium lignin peroxidase gene. / Walther, Isabelle; Kälin, M.; Reiser, Jakob; Suter, F.; Fritsche, B.; Saloheimo, Markku; Leisola, Matti; Teeri, Tuula; Knowles, Jonathan; Fiechter, A.

In: Gene, Vol. 70, No. 1, 1988, p. 127-137.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Molecular analysis of a Phanerochaete chrysosporium lignin peroxidase gene

AU - Walther, Isabelle

AU - Kälin, M.

AU - Reiser, Jakob

AU - Suter, F.

AU - Fritsche, B.

AU - Saloheimo, Markku

AU - Leisola, Matti

AU - Teeri, Tuula

AU - Knowles, Jonathan

AU - Fiechter, A.

PY - 1988

Y1 - 1988

N2 - The basidiomycete fungus Phanerochaete chrysosporium produces a number of extracellular peroxidases which appear to be important for lignin degradation. We present here the isolation and complete nucleotide (nt) sequence of a gene (Ipo) coding for lignin peroxidase (LPO), the coding region of which is identical to a Ipo cDNA sequence which had previously been described [M. Tien and C.-P.D. Tu, Nature 326 (1987) 520–523]. The deduced amino acid (aa) sequence corresponds to 372 aa residues and the coding region is interrupted by eight short introns that range m size from 50 to 62 nt. Southern blot experiments using the cloned Ipo gene as a hybridization probe revealed a complex restriction fragment pattern, indicating that there are a number of lpo-rElated nucleotide sequences present m P. chrysosporium DNA which cross-hybridize. We also present data on the in vivo expression of the Ipo genes and show that they are regulated at the RNA level and that the structure of the transcripts as judged from Sl experiments is complex. These data are consistent with the idea that there are a number of related Ipo genes in P. chrysosporium which constitute a gene family.

AB - The basidiomycete fungus Phanerochaete chrysosporium produces a number of extracellular peroxidases which appear to be important for lignin degradation. We present here the isolation and complete nucleotide (nt) sequence of a gene (Ipo) coding for lignin peroxidase (LPO), the coding region of which is identical to a Ipo cDNA sequence which had previously been described [M. Tien and C.-P.D. Tu, Nature 326 (1987) 520–523]. The deduced amino acid (aa) sequence corresponds to 372 aa residues and the coding region is interrupted by eight short introns that range m size from 50 to 62 nt. Southern blot experiments using the cloned Ipo gene as a hybridization probe revealed a complex restriction fragment pattern, indicating that there are a number of lpo-rElated nucleotide sequences present m P. chrysosporium DNA which cross-hybridize. We also present data on the in vivo expression of the Ipo genes and show that they are regulated at the RNA level and that the structure of the transcripts as judged from Sl experiments is complex. These data are consistent with the idea that there are a number of related Ipo genes in P. chrysosporium which constitute a gene family.

U2 - 10.1016/0378-1119(88)90111-4

DO - 10.1016/0378-1119(88)90111-4

M3 - Article

VL - 70

SP - 127

EP - 137

JO - Gene

JF - Gene

SN - 0378-1119

IS - 1

ER -