Molecular approaches for the detection and identification of Bifidobacteria and Lactobacilli in the human gastrointestinal tract

Reetta Satokari (Corresponding Author), E. E. Vaughan, H. Smidt, Maria Saarela, W. M. de Vos

    Research output: Contribution to journalArticleScientificpeer-review

    94 Citations (Scopus)

    Abstract

    In this review an overview of various molecular techniques and their application for the detection and identification of bifidobacteria and lactobacilli in the gastrointestinal (GI) tract is presented. The techniques include molecular typing techniques such as amplified ribosomal DNA restriction analysis (ARDRA), randomly amplified polymorphic DNA (RAPD), pulsed field gel electrophoresis (PFGE), ribotyping and community profiling techniques such as PCR coupled to temperature and denaturing gradient gel electrophoresis (PCR-TGGE and PCR-DGGE, respectively). Special attention is given to oligonucleotide probes and primers that target the ribosomal RNA (rRNA) sequences and their use in PCR and different hybridisation techniques such as DNA microarrays and fluorescent in situ hybridisation (FISH). In addition, recent findings based on the molecular studies of bifidobacteria and lactobacilli in the GI-tract are reviewed.
    Original languageEnglish
    Pages (from-to)572-584
    Number of pages13
    JournalSystematic and Applied Microbiology
    Volume26
    Issue number4
    DOIs
    Publication statusPublished - 2003
    MoE publication typeA1 Journal article-refereed

    Keywords

    • Lactobacillus
    • Bifidobacteria
    • Identification
    • Detection
    • Molecular techniques
    • rRNA

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