Molecular approaches for the detection and identification of Bifidobacteria and Lactobacilli in the human gastrointestinal tract

Reetta Satokari (Corresponding Author), E. E. Vaughan, H. Smidt, Maria Saarela, W. M. de Vos

Research output: Contribution to journalArticleScientificpeer-review

91 Citations (Scopus)

Abstract

In this review an overview of various molecular techniques and their application for the detection and identification of bifidobacteria and lactobacilli in the gastrointestinal (GI) tract is presented. The techniques include molecular typing techniques such as amplified ribosomal DNA restriction analysis (ARDRA), randomly amplified polymorphic DNA (RAPD), pulsed field gel electrophoresis (PFGE), ribotyping and community profiling techniques such as PCR coupled to temperature and denaturing gradient gel electrophoresis (PCR-TGGE and PCR-DGGE, respectively). Special attention is given to oligonucleotide probes and primers that target the ribosomal RNA (rRNA) sequences and their use in PCR and different hybridisation techniques such as DNA microarrays and fluorescent in situ hybridisation (FISH). In addition, recent findings based on the molecular studies of bifidobacteria and lactobacilli in the GI-tract are reviewed.
Original languageEnglish
Pages (from-to)572-584
Number of pages13
JournalSystematic and Applied Microbiology
Volume26
Issue number4
DOIs
Publication statusPublished - 2003
MoE publication typeA1 Journal article-refereed

Fingerprint

Bifidobacterium
Lactobacillus
gastrointestinal system
Gastrointestinal Tract
DNA
Polymerase Chain Reaction
Denaturing Gradient Gel Electrophoresis
electrokinesis
gel
denaturing gradient gel electrophoresis
Ribotyping
Molecular Typing
DNA Primers
Oligonucleotide Probes
Pulsed Field Gel Electrophoresis
Ribosomal DNA
Oligonucleotide Array Sequence Analysis
Fluorescence In Situ Hybridization
methodology
probe

Keywords

  • Lactobacillus
  • Bifidobacteria
  • Identification
  • Detection
  • Molecular techniques
  • rRNA

Cite this

Satokari, Reetta ; Vaughan, E. E. ; Smidt, H. ; Saarela, Maria ; de Vos, W. M. / Molecular approaches for the detection and identification of Bifidobacteria and Lactobacilli in the human gastrointestinal tract. In: Systematic and Applied Microbiology. 2003 ; Vol. 26, No. 4. pp. 572-584.
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Molecular approaches for the detection and identification of Bifidobacteria and Lactobacilli in the human gastrointestinal tract. / Satokari, Reetta (Corresponding Author); Vaughan, E. E.; Smidt, H.; Saarela, Maria; de Vos, W. M.

In: Systematic and Applied Microbiology, Vol. 26, No. 4, 2003, p. 572-584.

Research output: Contribution to journalArticleScientificpeer-review

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AU - de Vos, W. M.

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KW - Bifidobacteria

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