Molecular cloning of cellulolytic enzyme genes from Cellulomonas flavigena in E. Coli

Jesus Silva, Markku Saloheimo, Cecilia Montanez, Jonathan Knowles, Tuula Teeri

Research output: Contribution to journalArticleScientificpeer-review

5 Citations (Scopus)

Abstract

A genomic bank of Cellulomonas flavigena was constructed in E. coli using the pUC18 vector, and over 14000 clones screened for cellulolytic activity.
Three different cellulolytic enzyme genes were cloned, one coding for an endo-β-glucanase (pJS10, CMC activity) and two coding for β-glucosidases, each with a distinct substrate specificity (pJS3, X-glu, and pJS4, X-glu and MUC activities).
These three inserts have different restriction patterns to each other and the previously isolated cellulolytic enzyme genes from C. fimi and C. uda.
Original languageEnglish
Pages (from-to)249-255
JournalJournal of Biotechnology
Volume8
Issue number3
DOIs
Publication statusPublished - 1988
MoE publication typeA1 Journal article-refereed

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Cellulomonas
Cloning
Molecular Cloning
Escherichia coli
Enzymes
Genes
Glucosidases
Substrate Specificity
Clone Cells
Substrates

Cite this

Silva, Jesus ; Saloheimo, Markku ; Montanez, Cecilia ; Knowles, Jonathan ; Teeri, Tuula. / Molecular cloning of cellulolytic enzyme genes from Cellulomonas flavigena in E. Coli. In: Journal of Biotechnology. 1988 ; Vol. 8, No. 3. pp. 249-255.
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Molecular cloning of cellulolytic enzyme genes from Cellulomonas flavigena in E. Coli. / Silva, Jesus; Saloheimo, Markku; Montanez, Cecilia; Knowles, Jonathan; Teeri, Tuula.

In: Journal of Biotechnology, Vol. 8, No. 3, 1988, p. 249-255.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Molecular cloning of cellulolytic enzyme genes from Cellulomonas flavigena in E. Coli

AU - Silva, Jesus

AU - Saloheimo, Markku

AU - Montanez, Cecilia

AU - Knowles, Jonathan

AU - Teeri, Tuula

PY - 1988

Y1 - 1988

N2 - A genomic bank of Cellulomonas flavigena was constructed in E. coli using the pUC18 vector, and over 14000 clones screened for cellulolytic activity. Three different cellulolytic enzyme genes were cloned, one coding for an endo-β-glucanase (pJS10, CMC activity) and two coding for β-glucosidases, each with a distinct substrate specificity (pJS3, X-glu, and pJS4, X-glu and MUC activities). These three inserts have different restriction patterns to each other and the previously isolated cellulolytic enzyme genes from C. fimi and C. uda.

AB - A genomic bank of Cellulomonas flavigena was constructed in E. coli using the pUC18 vector, and over 14000 clones screened for cellulolytic activity. Three different cellulolytic enzyme genes were cloned, one coding for an endo-β-glucanase (pJS10, CMC activity) and two coding for β-glucosidases, each with a distinct substrate specificity (pJS3, X-glu, and pJS4, X-glu and MUC activities). These three inserts have different restriction patterns to each other and the previously isolated cellulolytic enzyme genes from C. fimi and C. uda.

U2 - 10.1016/0168-1656(88)90007-7

DO - 10.1016/0168-1656(88)90007-7

M3 - Article

VL - 8

SP - 249

EP - 255

JO - Journal of Biotechnology

JF - Journal of Biotechnology

SN - 0168-1656

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