Molecular cloning of cellulolytic enzyme genes from Cellulomonas flavigena in E. Coli

Jesus Silva, Markku Saloheimo, Cecilia Montanez, Jonathan Knowles, Tuula Teeri

Research output: Contribution to journalArticleScientificpeer-review

5 Citations (Scopus)


A genomic bank of Cellulomonas flavigena was constructed in E. coli using the pUC18 vector, and over 14000 clones screened for cellulolytic activity.
Three different cellulolytic enzyme genes were cloned, one coding for an endo-β-glucanase (pJS10, CMC activity) and two coding for β-glucosidases, each with a distinct substrate specificity (pJS3, X-glu, and pJS4, X-glu and MUC activities).
These three inserts have different restriction patterns to each other and the previously isolated cellulolytic enzyme genes from C. fimi and C. uda.
Original languageEnglish
Pages (from-to)249-255
JournalJournal of Biotechnology
Issue number3
Publication statusPublished - 1988
MoE publication typeA1 Journal article-refereed


Cite this