Molecular interactions between a recombinant IgE antibody and the beta-lactoglobulin allergen

Merja Niemi, Sirpa Jylhä, Marja-Leena Laukkanen, Hans Söderlund, Soili Mäkinen-Kiljunen, Johanna M. Kallio, Nina Hakulinen, Tari Haahtela, Kristiina Takkinen, Juha Rouvinen

Research output: Contribution to journalArticleScientificpeer-review

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Abstract

Allergies are caused by the immune reaction to commonly harmless proteins, allergens.
This reaction is typified by immunoglobulin E (IgE) antibodies. We report the crystal structure of an IgE Fab fragment in complex with β-lactoglobulin (BLG), one of the major allergens of bovine milk. The solved structure shows how two IgE/Fab molecules bind the dimeric BLG. The epitope of BLG consists of six different short fragments of the polypeptide chain, which are located especially in the β strands, covering a flat area on the allergen surface.
All six CDR (complementary-determining region) loops of the IgE Fab participate in the binding of BLG. The light chain CDR loops are responsible for the binding of the flat β sheet region of BLG.
The IgE epitope is different from common IgG epitopes that are normally located in the exposed loop regions of antigens and observed also in the two recently determined allergen-IgG complexes.
Original languageEnglish
Pages (from-to)1413-1421
JournalStructure
Volume15
Issue number11
DOIs
Publication statusPublished - 2007
MoE publication typeA1 Journal article-refereed

Fingerprint

Lactoglobulins
Allergens
Immunoglobulin E
Antibodies
Epitopes
Immunoglobulin G
Immunoglobulin Fab Fragments
Hypersensitivity
Milk
Light
Antigens
Peptides
Proteins

Keywords

  • allergen
  • allergy
  • IgE antibody
  • antibodies
  • recombinant antibodies
  • lactoglobulin

Cite this

Niemi, M., Jylhä, S., Laukkanen, M-L., Söderlund, H., Mäkinen-Kiljunen, S., Kallio, J. M., ... Rouvinen, J. (2007). Molecular interactions between a recombinant IgE antibody and the beta-lactoglobulin allergen. Structure, 15(11), 1413-1421. https://doi.org/10.1016/j.str.2007.09.012
Niemi, Merja ; Jylhä, Sirpa ; Laukkanen, Marja-Leena ; Söderlund, Hans ; Mäkinen-Kiljunen, Soili ; Kallio, Johanna M. ; Hakulinen, Nina ; Haahtela, Tari ; Takkinen, Kristiina ; Rouvinen, Juha. / Molecular interactions between a recombinant IgE antibody and the beta-lactoglobulin allergen. In: Structure. 2007 ; Vol. 15, No. 11. pp. 1413-1421.
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abstract = "Allergies are caused by the immune reaction to commonly harmless proteins, allergens. This reaction is typified by immunoglobulin E (IgE) antibodies. We report the crystal structure of an IgE Fab fragment in complex with β-lactoglobulin (BLG), one of the major allergens of bovine milk. The solved structure shows how two IgE/Fab molecules bind the dimeric BLG. The epitope of BLG consists of six different short fragments of the polypeptide chain, which are located especially in the β strands, covering a flat area on the allergen surface. All six CDR (complementary-determining region) loops of the IgE Fab participate in the binding of BLG. The light chain CDR loops are responsible for the binding of the flat β sheet region of BLG. The IgE epitope is different from common IgG epitopes that are normally located in the exposed loop regions of antigens and observed also in the two recently determined allergen-IgG complexes.",
keywords = "allergen, allergy, IgE antibody, antibodies, recombinant antibodies, lactoglobulin",
author = "Merja Niemi and Sirpa Jylh{\"a} and Marja-Leena Laukkanen and Hans S{\"o}derlund and Soili M{\"a}kinen-Kiljunen and Kallio, {Johanna M.} and Nina Hakulinen and Tari Haahtela and Kristiina Takkinen and Juha Rouvinen",
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Niemi, M, Jylhä, S, Laukkanen, M-L, Söderlund, H, Mäkinen-Kiljunen, S, Kallio, JM, Hakulinen, N, Haahtela, T, Takkinen, K & Rouvinen, J 2007, 'Molecular interactions between a recombinant IgE antibody and the beta-lactoglobulin allergen', Structure, vol. 15, no. 11, pp. 1413-1421. https://doi.org/10.1016/j.str.2007.09.012

Molecular interactions between a recombinant IgE antibody and the beta-lactoglobulin allergen. / Niemi, Merja; Jylhä, Sirpa; Laukkanen, Marja-Leena; Söderlund, Hans; Mäkinen-Kiljunen, Soili; Kallio, Johanna M.; Hakulinen, Nina; Haahtela, Tari; Takkinen, Kristiina; Rouvinen, Juha.

In: Structure, Vol. 15, No. 11, 2007, p. 1413-1421.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Molecular interactions between a recombinant IgE antibody and the beta-lactoglobulin allergen

AU - Niemi, Merja

AU - Jylhä, Sirpa

AU - Laukkanen, Marja-Leena

AU - Söderlund, Hans

AU - Mäkinen-Kiljunen, Soili

AU - Kallio, Johanna M.

AU - Hakulinen, Nina

AU - Haahtela, Tari

AU - Takkinen, Kristiina

AU - Rouvinen, Juha

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AB - Allergies are caused by the immune reaction to commonly harmless proteins, allergens. This reaction is typified by immunoglobulin E (IgE) antibodies. We report the crystal structure of an IgE Fab fragment in complex with β-lactoglobulin (BLG), one of the major allergens of bovine milk. The solved structure shows how two IgE/Fab molecules bind the dimeric BLG. The epitope of BLG consists of six different short fragments of the polypeptide chain, which are located especially in the β strands, covering a flat area on the allergen surface. All six CDR (complementary-determining region) loops of the IgE Fab participate in the binding of BLG. The light chain CDR loops are responsible for the binding of the flat β sheet region of BLG. The IgE epitope is different from common IgG epitopes that are normally located in the exposed loop regions of antigens and observed also in the two recently determined allergen-IgG complexes.

KW - allergen

KW - allergy

KW - IgE antibody

KW - antibodies

KW - recombinant antibodies

KW - lactoglobulin

U2 - 10.1016/j.str.2007.09.012

DO - 10.1016/j.str.2007.09.012

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JF - Structure

SN - 0969-2126

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Niemi M, Jylhä S, Laukkanen M-L, Söderlund H, Mäkinen-Kiljunen S, Kallio JM et al. Molecular interactions between a recombinant IgE antibody and the beta-lactoglobulin allergen. Structure. 2007;15(11):1413-1421. https://doi.org/10.1016/j.str.2007.09.012