Abstract
| Original language | English |
|---|---|
| Pages (from-to) | 127 - 134 |
| Number of pages | 8 |
| Journal | Biodegradation |
| Volume | 16 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 2005 |
| MoE publication type | A1 Journal article-refereed |
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Keywords
- bioremediation
- inoculation
- molecular monitoring
- naphthalene
- soil slurry
Cite this
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Monitoring of accelerated naphthalene-biodegradation in a bioaugmented soil slurry. / Piskonen, Reetta (Corresponding Author); Nyyssönen, Mari; Rajamäki, Tiina; Itävaara, Merja.
In: Biodegradation, Vol. 16, No. 2, 2005, p. 127 - 134.Research output: Contribution to journal › Article › Scientific › peer-review
TY - JOUR
T1 - Monitoring of accelerated naphthalene-biodegradation in a bioaugmented soil slurry
AU - Piskonen, Reetta
AU - Nyyssönen, Mari
AU - Rajamäki, Tiina
AU - Itävaara, Merja
PY - 2005
Y1 - 2005
N2 - The effect of microbial inoculation on the mineralization of naphthalene in a bioslurry treatment was evaluated in soil slurry microcosms. Inoculation by Pseudomonas putida G7 carrying the naphthalene dioxygenase (nahA) gene resulted in rapid mineralization of naphthalene, whereas indigenous microorganisms in the PAH-contaminated soil required a 28 h adaptation period before significant mineralization occurred. The number of nahA-like gene copies increased in both the inoculated and non-inoculated soil as mineralization proceeded, indicating selection towards naphthalene dioxygenase producing bacteria in the microbial community. In addition, 16S rRNA analysis by denaturing gradient gel electrophoresis (DGGE) analysis showed that significant selection occurred in the microbial community as a result of biodegradation. However, the indigenous soil bacteria were not able to compete with the P. putida G7 inoculum adapted to naphthalene biodegradation, even though the soil microbial community slightly suppressed naphthalene mineralization by P. putida G7.
AB - The effect of microbial inoculation on the mineralization of naphthalene in a bioslurry treatment was evaluated in soil slurry microcosms. Inoculation by Pseudomonas putida G7 carrying the naphthalene dioxygenase (nahA) gene resulted in rapid mineralization of naphthalene, whereas indigenous microorganisms in the PAH-contaminated soil required a 28 h adaptation period before significant mineralization occurred. The number of nahA-like gene copies increased in both the inoculated and non-inoculated soil as mineralization proceeded, indicating selection towards naphthalene dioxygenase producing bacteria in the microbial community. In addition, 16S rRNA analysis by denaturing gradient gel electrophoresis (DGGE) analysis showed that significant selection occurred in the microbial community as a result of biodegradation. However, the indigenous soil bacteria were not able to compete with the P. putida G7 inoculum adapted to naphthalene biodegradation, even though the soil microbial community slightly suppressed naphthalene mineralization by P. putida G7.
KW - bioremediation
KW - inoculation
KW - molecular monitoring
KW - naphthalene
KW - soil slurry
U2 - 10.1007/s10532-004-4893-9
DO - 10.1007/s10532-004-4893-9
M3 - Article
VL - 16
SP - 127
EP - 134
JO - Biodegradation
JF - Biodegradation
SN - 0923-9820
IS - 2
ER -