Monitoring of accelerated naphthalene-biodegradation in a bioaugmented soil slurry

Reetta Piskonen (Corresponding Author), Mari Nyyssönen, Tiina Rajamäki, Merja Itävaara

Research output: Contribution to journalArticleScientificpeer-review

41 Citations (Scopus)

Abstract

The effect of microbial inoculation on the mineralization of naphthalene in a bioslurry treatment was evaluated in soil slurry microcosms. Inoculation by Pseudomonas putida G7 carrying the naphthalene dioxygenase (nahA) gene resulted in rapid mineralization of naphthalene, whereas indigenous microorganisms in the PAH-contaminated soil required a 28 h adaptation period before significant mineralization occurred. The number of nahA-like gene copies increased in both the inoculated and non-inoculated soil as mineralization proceeded, indicating selection towards naphthalene dioxygenase producing bacteria in the microbial community. In addition, 16S rRNA analysis by denaturing gradient gel electrophoresis (DGGE) analysis showed that significant selection occurred in the microbial community as a result of biodegradation. However, the indigenous soil bacteria were not able to compete with the P. putida G7 inoculum adapted to naphthalene biodegradation, even though the soil microbial community slightly suppressed naphthalene mineralization by P. putida G7.
Original languageEnglish
Pages (from-to)127 - 134
Number of pages8
JournalBiodegradation
Volume16
Issue number2
DOIs
Publication statusPublished - 2005
MoE publication typeA1 Journal article-refereed

Keywords

  • bioremediation
  • inoculation
  • molecular monitoring
  • naphthalene
  • soil slurry

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