Abstract
The effect of microbial inoculation on the mineralization of naphthalene
in a bioslurry treatment was evaluated in soil slurry microcosms.
Inoculation by Pseudomonas putida G7 carrying the naphthalene dioxygenase (nahA)
gene resulted in rapid mineralization of naphthalene, whereas
indigenous microorganisms in the PAH-contaminated soil required a 28 h
adaptation period before significant mineralization occurred. The number
of nahA-like gene copies increased
in both the inoculated and non-inoculated soil as mineralization
proceeded, indicating selection towards naphthalene dioxygenase
producing bacteria in the microbial community. In addition, 16S rRNA
analysis by denaturing gradient gel electrophoresis (DGGE) analysis
showed that significant selection occurred in the microbial community as
a result of biodegradation. However, the indigenous soil bacteria were
not able to compete with the P. putida
G7 inoculum adapted to naphthalene biodegradation, even though the soil
microbial community slightly suppressed naphthalene mineralization by P. putida G7.
Original language | English |
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Pages (from-to) | 127 - 134 |
Number of pages | 8 |
Journal | Biodegradation |
Volume | 16 |
Issue number | 2 |
DOIs | |
Publication status | Published - 2005 |
MoE publication type | A1 Journal article-refereed |
Keywords
- bioremediation
- inoculation
- molecular monitoring
- naphthalene
- soil slurry