Monitoring of accelerated naphthalene-biodegradation in a bioaugmented soil slurry

Reetta Piskonen; Mari Nyyssönen; Tiina Rajamäki; Merja Itävaara

doi:10.1007/s10532-004-4893-9

Monitoring of accelerated naphthalene-biodegradation in a bioaugmented soil slurry

Reetta Piskonen (Corresponding Author), Mari Nyyssönen, Tiina Rajamäki, Merja Itävaara

Research output: Contribution to journal › Article › Scientific › peer-review

44 Citations (Scopus)

Abstract

The effect of microbial inoculation on the mineralization of naphthalene in a bioslurry treatment was evaluated in soil slurry microcosms. Inoculation by Pseudomonas putida G7 carrying the naphthalene dioxygenase (nahA) gene resulted in rapid mineralization of naphthalene, whereas indigenous microorganisms in the PAH-contaminated soil required a 28 h adaptation period before significant mineralization occurred. The number of nahA-like gene copies increased in both the inoculated and non-inoculated soil as mineralization proceeded, indicating selection towards naphthalene dioxygenase producing bacteria in the microbial community. In addition, 16S rRNA analysis by denaturing gradient gel electrophoresis (DGGE) analysis showed that significant selection occurred in the microbial community as a result of biodegradation. However, the indigenous soil bacteria were not able to compete with the P. putida G7 inoculum adapted to naphthalene biodegradation, even though the soil microbial community slightly suppressed naphthalene mineralization by P. putida G7.

Original language	English
Pages (from-to)	127 - 134
Number of pages	8
Journal	Biodegradation
Volume	16
Issue number	2
DOIs	https://doi.org/10.1007/s10532-004-4893-9
Publication status	Published - 2005
MoE publication type	A1 Journal article-refereed

Keywords

bioremediation
inoculation
molecular monitoring
naphthalene
soil slurry

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10.1007/s10532-004-4893-9

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title = "Monitoring of accelerated naphthalene-biodegradation in a bioaugmented soil slurry",

abstract = "The effect of microbial inoculation on the mineralization of naphthalene in a bioslurry treatment was evaluated in soil slurry microcosms. Inoculation by Pseudomonas putida G7 carrying the naphthalene dioxygenase (nahA) gene resulted in rapid mineralization of naphthalene, whereas indigenous microorganisms in the PAH-contaminated soil required a 28 h adaptation period before significant mineralization occurred. The number of nahA-like gene copies increased in both the inoculated and non-inoculated soil as mineralization proceeded, indicating selection towards naphthalene dioxygenase producing bacteria in the microbial community. In addition, 16S rRNA analysis by denaturing gradient gel electrophoresis (DGGE) analysis showed that significant selection occurred in the microbial community as a result of biodegradation. However, the indigenous soil bacteria were not able to compete with the P. putida G7 inoculum adapted to naphthalene biodegradation, even though the soil microbial community slightly suppressed naphthalene mineralization by P. putida G7.",

keywords = "bioremediation, inoculation, molecular monitoring, naphthalene, soil slurry",

author = "Reetta Piskonen and Mari Nyyss{\"o}nen and Tiina Rajam{\"a}ki and Merja It{\"a}vaara",

year = "2005",

doi = "10.1007/s10532-004-4893-9",

language = "English",

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journal = "Biodegradation",

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T1 - Monitoring of accelerated naphthalene-biodegradation in a bioaugmented soil slurry

AU - Piskonen, Reetta

AU - Nyyssönen, Mari

AU - Rajamäki, Tiina

AU - Itävaara, Merja

PY - 2005

Y1 - 2005

N2 - The effect of microbial inoculation on the mineralization of naphthalene in a bioslurry treatment was evaluated in soil slurry microcosms. Inoculation by Pseudomonas putida G7 carrying the naphthalene dioxygenase (nahA) gene resulted in rapid mineralization of naphthalene, whereas indigenous microorganisms in the PAH-contaminated soil required a 28 h adaptation period before significant mineralization occurred. The number of nahA-like gene copies increased in both the inoculated and non-inoculated soil as mineralization proceeded, indicating selection towards naphthalene dioxygenase producing bacteria in the microbial community. In addition, 16S rRNA analysis by denaturing gradient gel electrophoresis (DGGE) analysis showed that significant selection occurred in the microbial community as a result of biodegradation. However, the indigenous soil bacteria were not able to compete with the P. putida G7 inoculum adapted to naphthalene biodegradation, even though the soil microbial community slightly suppressed naphthalene mineralization by P. putida G7.

AB - The effect of microbial inoculation on the mineralization of naphthalene in a bioslurry treatment was evaluated in soil slurry microcosms. Inoculation by Pseudomonas putida G7 carrying the naphthalene dioxygenase (nahA) gene resulted in rapid mineralization of naphthalene, whereas indigenous microorganisms in the PAH-contaminated soil required a 28 h adaptation period before significant mineralization occurred. The number of nahA-like gene copies increased in both the inoculated and non-inoculated soil as mineralization proceeded, indicating selection towards naphthalene dioxygenase producing bacteria in the microbial community. In addition, 16S rRNA analysis by denaturing gradient gel electrophoresis (DGGE) analysis showed that significant selection occurred in the microbial community as a result of biodegradation. However, the indigenous soil bacteria were not able to compete with the P. putida G7 inoculum adapted to naphthalene biodegradation, even though the soil microbial community slightly suppressed naphthalene mineralization by P. putida G7.

KW - bioremediation

KW - inoculation

KW - molecular monitoring

KW - naphthalene

KW - soil slurry

U2 - 10.1007/s10532-004-4893-9

DO - 10.1007/s10532-004-4893-9

M3 - Article

VL - 16

SP - 127

EP - 134

JO - Biodegradation

JF - Biodegradation

SN - 0923-9820

IS - 2

ER -

Monitoring of accelerated naphthalene-biodegradation in a bioaugmented soil slurry

Abstract

Keywords

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