Monitoring of TiO2 and ZnO nanoparticle penetration into enamel and dentine of human tooth in vitro and assessment of their photocatalytic ability

Natalia A. Trunina, Maxim E. Darvin, Krisztían Kord́as, Anjana Sarkar, Jyri Pekka Mikkola, J̈urgen Lademann, Martina C. Meinke, Risto Myllyl̈a, Valery V. Tuchin, Alexey P. Popov

Research output: Contribution to journalArticleScientificpeer-review

5 Citations (Scopus)

Abstract

Penetration of nanoparticles into tooth enamel and dentine is of significant interest upon solving problems related to reduction of tooth sensitivity, enamel strengthening, disinfection, restoration as well as cosmetic bleaching. This paper aims at studying the process of nanoparticle penetration into tooth enamel and dentine samples using nonlinear optical microscopy and at investigating the influence of the same nanoparticles on the generation of free radicals using the electronic paramagnetic resonance technique. We presented in vitro measurements demonstrating that nonlinear optical microscopy, namely, two-photon-excited autofluorescence, second harmonic generation, and hyper-Rayleigh scattering-based microscopy can be used for monitoring and imaging TiO2 and ZnO nanoparticle penetration into tooth tissues. The results indicate that ZnO nanoparticles penetrated into the human tooth enamel and dentine up to a depth of 12 and 45 μm, respectively, and TiO2 nanoparticles penetrated into dentine to a depth of 5 μm. The penetration mainly occurs along either enamel rods or dentinal tubules. Permeability of the dentine was found to be higher than that of enamel (for ZnO particles) by one order ofmagnitude and the diffusion rate was affected by the particle size being higher for smaller, submicron particles (ZnO) than for micronsized aggregates (TiO2). Nitrogen-doped TiO2 nanoparticles generate more radicals in the UV-VIS spectral range in comparison to pristine TiO2 (anatase) and ZnO nanoparticles, therefore, they can potentially be used for disinfection purposes of superficial tooth areas (up to 5-μm deep).

Original languageEnglish
Article number30
JournalIEEE Journal on Selected Topics in Quantum Electronics
Volume20
Issue number3
DOIs
Publication statusPublished - 1 Jan 2014
MoE publication typeA1 Journal article-refereed

Keywords

  • Biological tissues
  • Biomedical optical imaging
  • Dentistry
  • Nanotechnology
  • Nonlinear optics
  • Paramagnetic resonance

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