Monoclonal antibodies against core and cellulose-binding domains of Trichoderma reesei cellobiohydrolases I and II and endoglucanase I

Sirpa Aho, Vesa Olkkonen, Taina Jalava, Marja Paloheimo, Rolf Buhler, Marja-Leena Niku-Paavola, Dennis Bamford (Corresponding Author), Matti Korhola

Research output: Contribution to journalArticleScientificpeer-review

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Abstract

Cellulases from Trichoderma reesei form an enzyme group with a common structural organization. Each cellulase enzyme is composed of two functional domains, the core region containing the active site and the cellulose‐binding domain (CBD). To facilitate the specific detection of each domain, monoclonal antibodies (mAb) against cellobiohydrolase I (CBHI), cellobiohydrolase II (CBHII) and endoglucanase I (EGI) were produced. Five mAb were obtained against CBHI, ten against CBHII and eight against EGI. The location of the antigenic epitope for each antibody was mapped by allowing the antibodies to react with truncated cellulases, synthesized from deleted cDNA in Saccharomyces cerevisiae. Proteolytic fragments of Trichoderma cellulases, obtained by papain digestion, were used to confirm the results. Specific antibodies were detected against the core and the CBD epitopes for all three cellulases. Using the truncated enzymes, it was possible to locate the epitopes to a reasonably short region within the protein. To obtain a quantitative assay for each enzyme, a specific mAb against each antigen was chosen, based on the affinity to the corresponding antigen on Western‐blot staining and on filter blots of the cellulolytic yeasts. The mAb were used to quantitative the corresponding enzymes in T. reesei culture medium. Specific quantitation of each cellulase enzyme has not been possible by biochemical assays or using polyclonal antibodies, due to their cross‐reactions. Now, these mAb can be specifically used to recognize and quantitate different domains of these three important cellulolytic enzymes.

Original languageEnglish
Pages (from-to)643 - 649
Number of pages7
JournalEuropean Journal of Biochemistry
Volume200
Issue number3
DOIs
Publication statusPublished - 1991
MoE publication typeA1 Journal article-refereed

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Cellulose 1,4-beta-Cellobiosidase
Cellulases
Trichoderma
Cellulose
Monoclonal Antibodies
Enzymes
Epitopes
Cellulase
Antibodies
Yeast
Assays
Antigens
Papain
Enzyme Assays
Saccharomyces cerevisiae
Culture Media
Digestion
Catalytic Domain
Complementary DNA
Yeasts

Cite this

Aho, Sirpa ; Olkkonen, Vesa ; Jalava, Taina ; Paloheimo, Marja ; Buhler, Rolf ; Niku-Paavola, Marja-Leena ; Bamford, Dennis ; Korhola, Matti. / Monoclonal antibodies against core and cellulose-binding domains of Trichoderma reesei cellobiohydrolases I and II and endoglucanase I. In: European Journal of Biochemistry. 1991 ; Vol. 200, No. 3. pp. 643 - 649.
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title = "Monoclonal antibodies against core and cellulose-binding domains of Trichoderma reesei cellobiohydrolases I and II and endoglucanase I",
abstract = "Cellulases from Trichoderma reesei form an enzyme group with a common structural organization. Each cellulase enzyme is composed of two functional domains, the core region containing the active site and the cellulose‐binding domain (CBD). To facilitate the specific detection of each domain, monoclonal antibodies (mAb) against cellobiohydrolase I (CBHI), cellobiohydrolase II (CBHII) and endoglucanase I (EGI) were produced. Five mAb were obtained against CBHI, ten against CBHII and eight against EGI. The location of the antigenic epitope for each antibody was mapped by allowing the antibodies to react with truncated cellulases, synthesized from deleted cDNA in Saccharomyces cerevisiae. Proteolytic fragments of Trichoderma cellulases, obtained by papain digestion, were used to confirm the results. Specific antibodies were detected against the core and the CBD epitopes for all three cellulases. Using the truncated enzymes, it was possible to locate the epitopes to a reasonably short region within the protein. To obtain a quantitative assay for each enzyme, a specific mAb against each antigen was chosen, based on the affinity to the corresponding antigen on Western‐blot staining and on filter blots of the cellulolytic yeasts. The mAb were used to quantitative the corresponding enzymes in T. reesei culture medium. Specific quantitation of each cellulase enzyme has not been possible by biochemical assays or using polyclonal antibodies, due to their cross‐reactions. Now, these mAb can be specifically used to recognize and quantitate different domains of these three important cellulolytic enzymes.",
author = "Sirpa Aho and Vesa Olkkonen and Taina Jalava and Marja Paloheimo and Rolf Buhler and Marja-Leena Niku-Paavola and Dennis Bamford and Matti Korhola",
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Aho, S, Olkkonen, V, Jalava, T, Paloheimo, M, Buhler, R, Niku-Paavola, M-L, Bamford, D & Korhola, M 1991, 'Monoclonal antibodies against core and cellulose-binding domains of Trichoderma reesei cellobiohydrolases I and II and endoglucanase I', European Journal of Biochemistry, vol. 200, no. 3, pp. 643 - 649. https://doi.org/10.1111/j.1432-1033.1991.tb16227.x

Monoclonal antibodies against core and cellulose-binding domains of Trichoderma reesei cellobiohydrolases I and II and endoglucanase I. / Aho, Sirpa; Olkkonen, Vesa; Jalava, Taina; Paloheimo, Marja; Buhler, Rolf; Niku-Paavola, Marja-Leena; Bamford, Dennis (Corresponding Author); Korhola, Matti.

In: European Journal of Biochemistry, Vol. 200, No. 3, 1991, p. 643 - 649.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Monoclonal antibodies against core and cellulose-binding domains of Trichoderma reesei cellobiohydrolases I and II and endoglucanase I

AU - Aho, Sirpa

AU - Olkkonen, Vesa

AU - Jalava, Taina

AU - Paloheimo, Marja

AU - Buhler, Rolf

AU - Niku-Paavola, Marja-Leena

AU - Bamford, Dennis

AU - Korhola, Matti

N1 - Project code: BIO9003

PY - 1991

Y1 - 1991

N2 - Cellulases from Trichoderma reesei form an enzyme group with a common structural organization. Each cellulase enzyme is composed of two functional domains, the core region containing the active site and the cellulose‐binding domain (CBD). To facilitate the specific detection of each domain, monoclonal antibodies (mAb) against cellobiohydrolase I (CBHI), cellobiohydrolase II (CBHII) and endoglucanase I (EGI) were produced. Five mAb were obtained against CBHI, ten against CBHII and eight against EGI. The location of the antigenic epitope for each antibody was mapped by allowing the antibodies to react with truncated cellulases, synthesized from deleted cDNA in Saccharomyces cerevisiae. Proteolytic fragments of Trichoderma cellulases, obtained by papain digestion, were used to confirm the results. Specific antibodies were detected against the core and the CBD epitopes for all three cellulases. Using the truncated enzymes, it was possible to locate the epitopes to a reasonably short region within the protein. To obtain a quantitative assay for each enzyme, a specific mAb against each antigen was chosen, based on the affinity to the corresponding antigen on Western‐blot staining and on filter blots of the cellulolytic yeasts. The mAb were used to quantitative the corresponding enzymes in T. reesei culture medium. Specific quantitation of each cellulase enzyme has not been possible by biochemical assays or using polyclonal antibodies, due to their cross‐reactions. Now, these mAb can be specifically used to recognize and quantitate different domains of these three important cellulolytic enzymes.

AB - Cellulases from Trichoderma reesei form an enzyme group with a common structural organization. Each cellulase enzyme is composed of two functional domains, the core region containing the active site and the cellulose‐binding domain (CBD). To facilitate the specific detection of each domain, monoclonal antibodies (mAb) against cellobiohydrolase I (CBHI), cellobiohydrolase II (CBHII) and endoglucanase I (EGI) were produced. Five mAb were obtained against CBHI, ten against CBHII and eight against EGI. The location of the antigenic epitope for each antibody was mapped by allowing the antibodies to react with truncated cellulases, synthesized from deleted cDNA in Saccharomyces cerevisiae. Proteolytic fragments of Trichoderma cellulases, obtained by papain digestion, were used to confirm the results. Specific antibodies were detected against the core and the CBD epitopes for all three cellulases. Using the truncated enzymes, it was possible to locate the epitopes to a reasonably short region within the protein. To obtain a quantitative assay for each enzyme, a specific mAb against each antigen was chosen, based on the affinity to the corresponding antigen on Western‐blot staining and on filter blots of the cellulolytic yeasts. The mAb were used to quantitative the corresponding enzymes in T. reesei culture medium. Specific quantitation of each cellulase enzyme has not been possible by biochemical assays or using polyclonal antibodies, due to their cross‐reactions. Now, these mAb can be specifically used to recognize and quantitate different domains of these three important cellulolytic enzymes.

U2 - 10.1111/j.1432-1033.1991.tb16227.x

DO - 10.1111/j.1432-1033.1991.tb16227.x

M3 - Article

VL - 200

SP - 643

EP - 649

JO - FEBS Journal

JF - FEBS Journal

SN - 1742-464X

IS - 3

ER -