Multifunctional hydrophobin

Toward functional coatings for drug nanoparticles

Hanna Valo (Corresponding Author), Päivi Laaksonen, Leena Peltonen, Markus Linder, Jouni Hirvonen, Timo Laaksonen

Research output: Contribution to journalArticleScientificpeer-review

90 Citations (Scopus)

Abstract

Efficient delivery of nanosized drug formulations to the desired body sites is not always reached despite the rapid development of pharmaceutical nanotechnologies. In spite of the undoubted effect of the size for increased bioavailability and controlled drug delivery, submicrometer formulations also require a deeper level of design. The surface properties of the particles determine the stability of the particles, interactions with the body, and targeting potentials of drugs. Thus, the efficacy of the drug can be increased utilizing the surface layer of the nanoparticles. Influencing the surface characters of the drug is the main focus of the present work, which introduces a method for preparing nanoparticles with functional sites from low-solubility drugs using hydrophobin (HFB) proteins. Particles were prepared by precipitating a lipophilic drug (beclomethasone dipropionate) in water in the presence of the HFB proteins. Particle size below 200 nm could easily be reached with increasing HFB concentration. The particles were shown to be stable for at least 5 h in suspension, and they could be stored for longer periods of time after freeze-drying. Labeling studies using green fluorescent protein (GFP) genetically fused to a HFB clearly demonstrated that the surface of the nanoparticles was covered with the hydrophobins and that the surface could be further modified by utilizing fusion proteins. This provides a template for a variety of different functional surface-bound groups that could be tailored by modifying the hydrophilic side of the HFB via protein bioengineering. In this study, the combination of proteins and traditional pharmaceutical technology was used to synthesize functionalized protein-coated nanoparticles for drug delivery purposes.
Original languageEnglish
Pages (from-to)1750-1758
JournalACS Nano
Volume4
Issue number3
DOIs
Publication statusPublished - 2010
MoE publication typeA1 Journal article-refereed

Fingerprint

drugs
Nanoparticles
Proteins
coatings
Coatings
nanoparticles
proteins
Pharmaceutical Preparations
delivery
Drug products
Controlled drug delivery
Beclomethasone
bioengineering
Particle interactions
formulations
bioavailability
freeze drying
Green Fluorescent Proteins
Drug delivery
Nanotechnology

Keywords

  • nanoparticles
  • hydrophobins
  • fusion proteins
  • beclomethasone dipropionate
  • labeling
  • functionalization

Cite this

Valo, H., Laaksonen, P., Peltonen, L., Linder, M., Hirvonen, J., & Laaksonen, T. (2010). Multifunctional hydrophobin: Toward functional coatings for drug nanoparticles. ACS Nano, 4(3), 1750-1758. https://doi.org/10.1021/nn9017558
Valo, Hanna ; Laaksonen, Päivi ; Peltonen, Leena ; Linder, Markus ; Hirvonen, Jouni ; Laaksonen, Timo. / Multifunctional hydrophobin : Toward functional coatings for drug nanoparticles. In: ACS Nano. 2010 ; Vol. 4, No. 3. pp. 1750-1758.
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Valo, H, Laaksonen, P, Peltonen, L, Linder, M, Hirvonen, J & Laaksonen, T 2010, 'Multifunctional hydrophobin: Toward functional coatings for drug nanoparticles', ACS Nano, vol. 4, no. 3, pp. 1750-1758. https://doi.org/10.1021/nn9017558

Multifunctional hydrophobin : Toward functional coatings for drug nanoparticles. / Valo, Hanna (Corresponding Author); Laaksonen, Päivi; Peltonen, Leena; Linder, Markus; Hirvonen, Jouni; Laaksonen, Timo.

In: ACS Nano, Vol. 4, No. 3, 2010, p. 1750-1758.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Multifunctional hydrophobin

T2 - Toward functional coatings for drug nanoparticles

AU - Valo, Hanna

AU - Laaksonen, Päivi

AU - Peltonen, Leena

AU - Linder, Markus

AU - Hirvonen, Jouni

AU - Laaksonen, Timo

PY - 2010

Y1 - 2010

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AB - Efficient delivery of nanosized drug formulations to the desired body sites is not always reached despite the rapid development of pharmaceutical nanotechnologies. In spite of the undoubted effect of the size for increased bioavailability and controlled drug delivery, submicrometer formulations also require a deeper level of design. The surface properties of the particles determine the stability of the particles, interactions with the body, and targeting potentials of drugs. Thus, the efficacy of the drug can be increased utilizing the surface layer of the nanoparticles. Influencing the surface characters of the drug is the main focus of the present work, which introduces a method for preparing nanoparticles with functional sites from low-solubility drugs using hydrophobin (HFB) proteins. Particles were prepared by precipitating a lipophilic drug (beclomethasone dipropionate) in water in the presence of the HFB proteins. Particle size below 200 nm could easily be reached with increasing HFB concentration. The particles were shown to be stable for at least 5 h in suspension, and they could be stored for longer periods of time after freeze-drying. Labeling studies using green fluorescent protein (GFP) genetically fused to a HFB clearly demonstrated that the surface of the nanoparticles was covered with the hydrophobins and that the surface could be further modified by utilizing fusion proteins. This provides a template for a variety of different functional surface-bound groups that could be tailored by modifying the hydrophilic side of the HFB via protein bioengineering. In this study, the combination of proteins and traditional pharmaceutical technology was used to synthesize functionalized protein-coated nanoparticles for drug delivery purposes.

KW - nanoparticles

KW - hydrophobins

KW - fusion proteins

KW - beclomethasone dipropionate

KW - labeling

KW - functionalization

U2 - 10.1021/nn9017558

DO - 10.1021/nn9017558

M3 - Article

VL - 4

SP - 1750

EP - 1758

JO - ACS Nano

JF - ACS Nano

SN - 1936-0851

IS - 3

ER -

Valo H, Laaksonen P, Peltonen L, Linder M, Hirvonen J, Laaksonen T. Multifunctional hydrophobin: Toward functional coatings for drug nanoparticles. ACS Nano. 2010;4(3):1750-1758. https://doi.org/10.1021/nn9017558