Multiplex protein detection with DNA readout via mass spectrometry

James Flanigon (Corresponding Author), Masood Kamali-Moghaddam, Ian Burbulis, Carla Annink, Martin Steffen, Paul Oeth, Roger Brent, Dirk van den Boom, Ulf Landegren, Charles Cantor

Research output: Contribution to journalArticleScientificpeer-review

6 Citations (Scopus)

Abstract

Multiplex protein quantification has been constrained by issues of assay specificity, sensitivity and throughput. This research presents a novel approach that overcomes these limitations using antibody–oligonucleotide conjugates for immuno-polymerase chain reaction (immuno-PCR) or proximity ligation, coupled with competitive PCR and MALDI-TOF mass spectrometry. Employing these combinations of technologies, we demonstrate multiplex detection and quantification of up to eight proteins, spanning wide dynamic ranges from femtomolar concentrations, using only microliter sample volumes.
Original languageEnglish
Pages (from-to)153-158
JournalNew Biotechnology
Volume30
Issue number2
DOIs
Publication statusPublished - 2013
MoE publication typeA1 Journal article-refereed

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Mass spectrometry
Mass Spectrometry
DNA
Proteins
Polymerase Chain Reaction
Polymerase chain reaction
Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
Ligation
Assays
Throughput
Technology
Sensitivity and Specificity
Research

Cite this

Flanigon, J., Kamali-Moghaddam, M., Burbulis, I., Annink, C., Steffen, M., Oeth, P., ... Cantor, C. (2013). Multiplex protein detection with DNA readout via mass spectrometry. New Biotechnology, 30(2), 153-158. https://doi.org/10.1016/j.nbt.2012.11.003
Flanigon, James ; Kamali-Moghaddam, Masood ; Burbulis, Ian ; Annink, Carla ; Steffen, Martin ; Oeth, Paul ; Brent, Roger ; van den Boom, Dirk ; Landegren, Ulf ; Cantor, Charles. / Multiplex protein detection with DNA readout via mass spectrometry. In: New Biotechnology. 2013 ; Vol. 30, No. 2. pp. 153-158.
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Flanigon, J, Kamali-Moghaddam, M, Burbulis, I, Annink, C, Steffen, M, Oeth, P, Brent, R, van den Boom, D, Landegren, U & Cantor, C 2013, 'Multiplex protein detection with DNA readout via mass spectrometry', New Biotechnology, vol. 30, no. 2, pp. 153-158. https://doi.org/10.1016/j.nbt.2012.11.003

Multiplex protein detection with DNA readout via mass spectrometry. / Flanigon, James (Corresponding Author); Kamali-Moghaddam, Masood; Burbulis, Ian; Annink, Carla; Steffen, Martin; Oeth, Paul; Brent, Roger; van den Boom, Dirk; Landegren, Ulf; Cantor, Charles.

In: New Biotechnology, Vol. 30, No. 2, 2013, p. 153-158.

Research output: Contribution to journalArticleScientificpeer-review

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AU - Flanigon, James

AU - Kamali-Moghaddam, Masood

AU - Burbulis, Ian

AU - Annink, Carla

AU - Steffen, Martin

AU - Oeth, Paul

AU - Brent, Roger

AU - van den Boom, Dirk

AU - Landegren, Ulf

AU - Cantor, Charles

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AB - Multiplex protein quantification has been constrained by issues of assay specificity, sensitivity and throughput. This research presents a novel approach that overcomes these limitations using antibody–oligonucleotide conjugates for immuno-polymerase chain reaction (immuno-PCR) or proximity ligation, coupled with competitive PCR and MALDI-TOF mass spectrometry. Employing these combinations of technologies, we demonstrate multiplex detection and quantification of up to eight proteins, spanning wide dynamic ranges from femtomolar concentrations, using only microliter sample volumes.

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DO - 10.1016/j.nbt.2012.11.003

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JF - New Biotechnology

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Flanigon J, Kamali-Moghaddam M, Burbulis I, Annink C, Steffen M, Oeth P et al. Multiplex protein detection with DNA readout via mass spectrometry. New Biotechnology. 2013;30(2):153-158. https://doi.org/10.1016/j.nbt.2012.11.003