Multiplex protein detection with DNA readout via mass spectrometry

James Flanigon (Corresponding Author), Masood Kamali-Moghaddam, Ian Burbulis, Carla Annink, Martin Steffen, Paul Oeth, Roger Brent, Dirk van den Boom, Ulf Landegren, Charles Cantor

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6 Citations (Scopus)

Abstract

Multiplex protein quantification has been constrained by issues of assay specificity, sensitivity and throughput. This research presents a novel approach that overcomes these limitations using antibody–oligonucleotide conjugates for immuno-polymerase chain reaction (immuno-PCR) or proximity ligation, coupled with competitive PCR and MALDI-TOF mass spectrometry. Employing these combinations of technologies, we demonstrate multiplex detection and quantification of up to eight proteins, spanning wide dynamic ranges from femtomolar concentrations, using only microliter sample volumes.
Original languageEnglish
Pages (from-to)153-158
JournalNew Biotechnology
Volume30
Issue number2
DOIs
Publication statusPublished - 2013
MoE publication typeA1 Journal article-refereed

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    Flanigon, J., Kamali-Moghaddam, M., Burbulis, I., Annink, C., Steffen, M., Oeth, P., Brent, R., van den Boom, D., Landegren, U., & Cantor, C. (2013). Multiplex protein detection with DNA readout via mass spectrometry. New Biotechnology, 30(2), 153-158. https://doi.org/10.1016/j.nbt.2012.11.003