Multiplex protein detection with DNA readout via mass spectrometry

James Flanigon; Masood Kamali-Moghaddam; Ian Burbulis; Carla Annink; Martin Steffen; Paul Oeth; Roger Brent; Dirk van den Boom; Ulf Landegren; Charles Cantor

doi:10.1016/j.nbt.2012.11.003

Multiplex protein detection with DNA readout via mass spectrometry

James Flanigon (Corresponding Author), Masood Kamali-Moghaddam, Ian Burbulis, Carla Annink, Martin Steffen, Paul Oeth, Roger Brent, Dirk van den Boom, Ulf Landegren, Charles Cantor

VTT Technical Research Centre of Finland

Research output: Contribution to journal › Article › Scientific › peer-review

8 Citations (Scopus)

Abstract

Multiplex protein quantification has been constrained by issues of assay specificity, sensitivity and throughput. This research presents a novel approach that overcomes these limitations using antibody–oligonucleotide conjugates for immuno-polymerase chain reaction (immuno-PCR) or proximity ligation, coupled with competitive PCR and MALDI-TOF mass spectrometry. Employing these combinations of technologies, we demonstrate multiplex detection and quantification of up to eight proteins, spanning wide dynamic ranges from femtomolar concentrations, using only microliter sample volumes.

Original language	English
Pages (from-to)	153-158
Journal	New Biotechnology
Volume	30
Issue number	2
DOIs	https://doi.org/10.1016/j.nbt.2012.11.003
Publication status	Published - 2013
MoE publication type	A1 Journal article-refereed

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10.1016/j.nbt.2012.11.003

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title = "Multiplex protein detection with DNA readout via mass spectrometry",

abstract = "Multiplex protein quantification has been constrained by issues of assay specificity, sensitivity and throughput. This research presents a novel approach that overcomes these limitations using antibody–oligonucleotide conjugates for immuno-polymerase chain reaction (immuno-PCR) or proximity ligation, coupled with competitive PCR and MALDI-TOF mass spectrometry. Employing these combinations of technologies, we demonstrate multiplex detection and quantification of up to eight proteins, spanning wide dynamic ranges from femtomolar concentrations, using only microliter sample volumes.",

author = "James Flanigon and Masood Kamali-Moghaddam and Ian Burbulis and Carla Annink and Martin Steffen and Paul Oeth and Roger Brent and {van den Boom}, Dirk and Ulf Landegren and Charles Cantor",

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AU - Flanigon, James

AU - Kamali-Moghaddam, Masood

AU - Burbulis, Ian

AU - Annink, Carla

AU - Steffen, Martin

AU - Oeth, Paul

AU - Brent, Roger

AU - van den Boom, Dirk

AU - Landegren, Ulf

AU - Cantor, Charles

PY - 2013

Y1 - 2013

N2 - Multiplex protein quantification has been constrained by issues of assay specificity, sensitivity and throughput. This research presents a novel approach that overcomes these limitations using antibody–oligonucleotide conjugates for immuno-polymerase chain reaction (immuno-PCR) or proximity ligation, coupled with competitive PCR and MALDI-TOF mass spectrometry. Employing these combinations of technologies, we demonstrate multiplex detection and quantification of up to eight proteins, spanning wide dynamic ranges from femtomolar concentrations, using only microliter sample volumes.

AB - Multiplex protein quantification has been constrained by issues of assay specificity, sensitivity and throughput. This research presents a novel approach that overcomes these limitations using antibody–oligonucleotide conjugates for immuno-polymerase chain reaction (immuno-PCR) or proximity ligation, coupled with competitive PCR and MALDI-TOF mass spectrometry. Employing these combinations of technologies, we demonstrate multiplex detection and quantification of up to eight proteins, spanning wide dynamic ranges from femtomolar concentrations, using only microliter sample volumes.

U2 - 10.1016/j.nbt.2012.11.003

DO - 10.1016/j.nbt.2012.11.003

M3 - Article

SN - 1871-6784

VL - 30

SP - 153

EP - 158

JO - New Biotechnology

JF - New Biotechnology

IS - 2

ER -

Multiplex protein detection with DNA readout via mass spectrometry

Abstract

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