TY - JOUR
T1 - Multiplexed mycotoxins determination employing white light reflectance spectroscopy and silicon chips with silicon oxide areas of different thickness
AU - Anastasiadis, Vasileios
AU - Koukouvinos, Georgios
AU - Petrou, Panagiota S.
AU - Economou, Anastasios
AU - Dekker, James
AU - Harjanne, Mikko
AU - Heimala, Paivi
AU - Goustouridis, Dimitris
AU - Raptis, Ioannis
AU - Kakabakos, Sotirios E.
N1 - Funding Information:
We acknowledge support of this work by the project “ NCSRD-INRASTES research activities in the framework of the national RIS3 ” ( MIS 5002559 ) which is implemented under the “Action for the Strategic Development on the Research and Technological Sector”, funded by the Operational Programme “ Competitiveness, Entrepreneurship and Innovation ” ( NSRF 2014–2020 ) and co-financed by Greece and the European Union (European Regional Development Fund) . This work was partially supported by EU project ACTPHAST (Grant Agreement No. 619205 ). We would like to thank ATPr&d S.r.l. (Via Ca’ Marzare, 3, 36043 Camisano Vicentino, Italy) for providing cereal samples with no detectable levels of AFB 1 and FB 1 as determined by an external laboratory.
Funding Information:
We acknowledge support of this work by the project ?NCSRD-INRASTES research activities in the framework of the national RIS3? (MIS 5002559) which is implemented under the ?Action for the Strategic Development on the Research and Technological Sector?, funded by the Operational Programme ?Competitiveness, Entrepreneurship and Innovation? (NSRF 2014?2020) and co-financed by Greece and the European Union (European Regional Development Fund). This work was partially supported by EU project ACTPHAST (Grant Agreement No. 619205). We would like to thank ATPr&d S.r.l. (Via Ca? Marzare, 3, 36043 Camisano Vicentino, Italy) for providing cereal samples with no detectable levels of AFB1 and FB1 as determined by an external laboratory.
Publisher Copyright:
© 2020 Elsevier B.V.
Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.
PY - 2020/4/1
Y1 - 2020/4/1
N2 - Biosensing through White Light Reflectance Spectroscopy (WLRS) is based on monitoring the shift of interference spectrum due to the binding reactions occurring on top of a thin SiO2 layer deposited on a silicon chip. Multi-analyte determinations were possible through scanning of a single sensor chip on which multiple bioreactive areas have been created. Nonetheless, the implementation of moving parts increased the instrumentation size and complexity and limited the potential for on-site determinations. Thus, in this work, a new approach, which is based on patterning the sensor surface to create areas with different SiO2 thickness, is developed and evaluated for multi-analyte determinations with the WLRS set-up. The areas of different thickness can be interrogated by a single reflection probe placed on a fixed position over the chip and the reflection spectrum recorded is de-convoluted to the spectra corresponding to each area allowing the simultaneous monitoring of the bioreactions taking place at each one of them. The combination of different areas thickness was optimized using chips with two areas for single analyte assays. The optimum chips were then used for the simultaneous determination of two mycotoxins, aflatoxin B1 and fumonisin B1. A competitive immunoassay format was followed employing immobilization of mycotoxin-protein conjugates onto the SiO2 of different thickness. It was found that the dual-analyte assays had identical analytical characteristics with the respective single-analyte ones. The detection limits achieved were 0.05 ng/mL for aflatoxin B1 and 1.0 ng/mL for fumonisin B1, with dynamic ranges extending up to 5.0 and 50 ng/mL, respectively. The sensor was also evaluated for the determination of the two mycotoxins in whole grain samples (wheat and maize). The extraction protocol was optimized and recoveries ranging from 85 to 115% have been determined. Due to lack of moving parts, the novel multi-analyte format is expected to considerably facilitate the built-up of a portable device for determination of analytes at the point-of-need.
AB - Biosensing through White Light Reflectance Spectroscopy (WLRS) is based on monitoring the shift of interference spectrum due to the binding reactions occurring on top of a thin SiO2 layer deposited on a silicon chip. Multi-analyte determinations were possible through scanning of a single sensor chip on which multiple bioreactive areas have been created. Nonetheless, the implementation of moving parts increased the instrumentation size and complexity and limited the potential for on-site determinations. Thus, in this work, a new approach, which is based on patterning the sensor surface to create areas with different SiO2 thickness, is developed and evaluated for multi-analyte determinations with the WLRS set-up. The areas of different thickness can be interrogated by a single reflection probe placed on a fixed position over the chip and the reflection spectrum recorded is de-convoluted to the spectra corresponding to each area allowing the simultaneous monitoring of the bioreactions taking place at each one of them. The combination of different areas thickness was optimized using chips with two areas for single analyte assays. The optimum chips were then used for the simultaneous determination of two mycotoxins, aflatoxin B1 and fumonisin B1. A competitive immunoassay format was followed employing immobilization of mycotoxin-protein conjugates onto the SiO2 of different thickness. It was found that the dual-analyte assays had identical analytical characteristics with the respective single-analyte ones. The detection limits achieved were 0.05 ng/mL for aflatoxin B1 and 1.0 ng/mL for fumonisin B1, with dynamic ranges extending up to 5.0 and 50 ng/mL, respectively. The sensor was also evaluated for the determination of the two mycotoxins in whole grain samples (wheat and maize). The extraction protocol was optimized and recoveries ranging from 85 to 115% have been determined. Due to lack of moving parts, the novel multi-analyte format is expected to considerably facilitate the built-up of a portable device for determination of analytes at the point-of-need.
KW - Aflatoxin B
KW - Cereal samples
KW - Fumonisin B
KW - Multi area reflectance spectroscopy
KW - Multiplexed detection
UR - http://www.scopus.com/inward/record.url?scp=85078203301&partnerID=8YFLogxK
U2 - 10.1016/j.bios.2020.112035
DO - 10.1016/j.bios.2020.112035
M3 - Article
C2 - 31989941
AN - SCOPUS:85078203301
SN - 0956-5663
VL - 153
JO - Biosensors & Bioelectronics
JF - Biosensors & Bioelectronics
M1 - 112035
ER -