Abstract
The present study investigated and compared the mycotoxin production of two Fusarium species, F. sporotrichioides and F. langsethiae, isolated from grain samples. Fusarium
strains were cultivated at 25°C for 7 days on two types of solid media,
i.e. rice-flour and cereal-flour agar. Toxins produced were measured
after the incubation period with a multi-mycotoxin method based on
liquid chromatography–tandem mass spectrometry (LC-MS/MS). Both F. sporotrichioides and F. langsethiae
synthesised type-A trichothecenes, i.e. T-2 and HT-2 toxins,
diacetoxyscirpenol (DAS) and neosolaniol (NEO). In addition, both
species could be verified as beauvericin producers. The toxin production
occurred in both cereal-based assays but was more predominant on the
carbohydrate-rich rice-flour medium. The two species were potent
producers of T-2 toxin, the highest amounts measured being at a level of
20,000 μg/kg after 7 days’ incubation. Differences between the species
were observed regarding the quantitative production of the other
trichothecenes: F. sporotrichioides was a more prolific producer of HT-2 toxin and beauvericin, whereas F. langsethiae
produced higher amounts of DAS and NEO. On rice-flour assay, the toxin
production was monitored during the growth period. The production
started rapidly at an early growth phase and several toxins could be
detected already after the 1st day of incubation, the highest
concentrations being at mg/kg level. The results also indicated that the
biosynthesis by F. sporotrichioides and F. langsethiae shifted towards the other type-A trichothecenes at the expense of T-2 toxin at the end of the cultivation.
Original language | English |
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Pages (from-to) | 25-35 |
Number of pages | 11 |
Journal | Mycotoxin Research |
Volume | 28 |
Issue number | 1 |
DOIs | |
Publication status | Published - 2012 |
MoE publication type | A1 Journal article-refereed |
Keywords
- Cereal
- Fusarium
- LC-MS/MS
- mycotoxin production
- substrate