NADPH-dependent 5-keto-D-gluconate reductase is a part of the fungal pathway for D-glucuronate catabolism

Joosu Kuivanen, Peter Richard

Research output: Contribution to journalArticleScientificpeer-review

4 Citations (Scopus)

Abstract

NADPH-dependent 5-keto-D-gluconate reductase was identified as a missing element in the pathway for D-glucuronate catabolism in fungi. The disruption of the gene, gluF, by CRISPR/Cas9 in the filamentous fungus Aspergillus niger resulted in a strain unable to catabolise D-glucuronate. The purified GluF protein was characterized and kcat and Km values of 23.7 ± 1.8 s-1 and 3.2 ± 0.1 mm for 5-keto-D-gluconate, respectively, were determined. The enzyme is reversible and is active with NADP+ and D-gluconate. We suggest a pathway for D-glucuronate catabolism with the intermediates L-gulonate, 2-keto-L-gulonate, L-idonate, 5-keto-D-gluconate, D-gluconate and D-gluconate-6-phosphate which is a part of the pentose phosphate pathway. A fungal enzyme activity for the conversion of L-gulonate to 2-keto-L-gulonate remains to be identified.

Original languageEnglish
Pages (from-to)71-77
Number of pages7
JournalFEBS Letters
Volume592
Issue number1
DOIs
Publication statusPublished - 1 Jan 2018
MoE publication typeA1 Journal article-refereed

Keywords

  • 5-keto-gluconate
  • Aspergillus niger
  • CRISPR/Cas9
  • D-gluconate-5-dehydrogenase
  • D-glucuronate
  • EC 1.1.1.69
  • Fungal pathway

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