Nanofibrillar cellulose hydrogel promotes three-dimensional liver cell culture

M. Bhattacharya, M. M. Malinen, P. Lauren, Y.-R. Lou, S. W. Kuisma, L. Kanninen, Martina Lille, A. Corlu, C. GuGuen-Guillouzo, O. Ikkala, A. Laukkanen, A. Urtti, M. Yliperttula (Corresponding Author)

Research output: Contribution to journalArticleScientificpeer-review

164 Citations (Scopus)

Abstract

Over the recent years, various materials have been introduced as potential 3D cell culture scaffolds. These include protein extracts, peptide amphiphiles, and synthetic polymers. Hydrogel scaffolds without human or animal borne components or added bioactive components are preferred from the immunological point of view. Here we demonstrate that native nanofibrillar cellulose (NFC) hydrogels derived from the abundant plant sources provide the desired functionalities. We show 1) rheological properties that allow formation of a 3D scaffold in-situ after facile injection, 2) cellular biocompatibility without added growth factors, 3) cellular polarization, and 4) differentiation of human hepatic cell lines HepaRG and HepG2. At high shear stress, the aqueous NFC has small viscosity that supports injectability, whereas at low shear stress conditions the material is converted to an elastic gel. Due to the inherent biocompatibility without any additives, we conclude that NFC generates a feasible and sustained microenvironment for 3D cell culture for potential applications, such as drug and chemical testing, tissue engineering, and cell therapy.
Original languageEnglish
Pages (from-to)291-298
Number of pages8
JournalJournal of Controlled Release
Volume164
Issue number3
DOIs
Publication statusPublished - 2012
MoE publication typeA1 Journal article-refereed
Event12th European Symposium of Controlled Drug Delivery - Egmond aan Zee, Netherlands
Duration: 4 Apr 20126 Apr 2012

Fingerprint

Hydrogel
Cellulose
Cell Culture Techniques
Cell- and Tissue-Based Therapy
Liver
Hydrogels
Tissue Engineering
Viscosity
Hepatocytes
Intercellular Signaling Peptides and Proteins
Polymers
Gels
Cell Line
Peptides
Injections
Pharmaceutical Preparations
Proteins

Keywords

  • 3D cell culture
  • Hepatocyte
  • Nanocellulose
  • Nanofiber
  • Plant-derived cellulose

Cite this

Bhattacharya, M., Malinen, M. M., Lauren, P., Lou, Y-R., Kuisma, S. W., Kanninen, L., ... Yliperttula, M. (2012). Nanofibrillar cellulose hydrogel promotes three-dimensional liver cell culture. Journal of Controlled Release, 164(3), 291-298. https://doi.org/10.1016/j.jconrel.2012.06.039
Bhattacharya, M. ; Malinen, M. M. ; Lauren, P. ; Lou, Y.-R. ; Kuisma, S. W. ; Kanninen, L. ; Lille, Martina ; Corlu, A. ; GuGuen-Guillouzo, C. ; Ikkala, O. ; Laukkanen, A. ; Urtti, A. ; Yliperttula, M. / Nanofibrillar cellulose hydrogel promotes three-dimensional liver cell culture. In: Journal of Controlled Release. 2012 ; Vol. 164, No. 3. pp. 291-298.
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abstract = "Over the recent years, various materials have been introduced as potential 3D cell culture scaffolds. These include protein extracts, peptide amphiphiles, and synthetic polymers. Hydrogel scaffolds without human or animal borne components or added bioactive components are preferred from the immunological point of view. Here we demonstrate that native nanofibrillar cellulose (NFC) hydrogels derived from the abundant plant sources provide the desired functionalities. We show 1) rheological properties that allow formation of a 3D scaffold in-situ after facile injection, 2) cellular biocompatibility without added growth factors, 3) cellular polarization, and 4) differentiation of human hepatic cell lines HepaRG and HepG2. At high shear stress, the aqueous NFC has small viscosity that supports injectability, whereas at low shear stress conditions the material is converted to an elastic gel. Due to the inherent biocompatibility without any additives, we conclude that NFC generates a feasible and sustained microenvironment for 3D cell culture for potential applications, such as drug and chemical testing, tissue engineering, and cell therapy.",
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Bhattacharya, M, Malinen, MM, Lauren, P, Lou, Y-R, Kuisma, SW, Kanninen, L, Lille, M, Corlu, A, GuGuen-Guillouzo, C, Ikkala, O, Laukkanen, A, Urtti, A & Yliperttula, M 2012, 'Nanofibrillar cellulose hydrogel promotes three-dimensional liver cell culture', Journal of Controlled Release, vol. 164, no. 3, pp. 291-298. https://doi.org/10.1016/j.jconrel.2012.06.039

Nanofibrillar cellulose hydrogel promotes three-dimensional liver cell culture. / Bhattacharya, M.; Malinen, M. M.; Lauren, P.; Lou, Y.-R.; Kuisma, S. W.; Kanninen, L.; Lille, Martina; Corlu, A.; GuGuen-Guillouzo, C.; Ikkala, O.; Laukkanen, A.; Urtti, A.; Yliperttula, M. (Corresponding Author).

In: Journal of Controlled Release, Vol. 164, No. 3, 2012, p. 291-298.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Nanofibrillar cellulose hydrogel promotes three-dimensional liver cell culture

AU - Bhattacharya, M.

AU - Malinen, M. M.

AU - Lauren, P.

AU - Lou, Y.-R.

AU - Kuisma, S. W.

AU - Kanninen, L.

AU - Lille, Martina

AU - Corlu, A.

AU - GuGuen-Guillouzo, C.

AU - Ikkala, O.

AU - Laukkanen, A.

AU - Urtti, A.

AU - Yliperttula, M.

PY - 2012

Y1 - 2012

N2 - Over the recent years, various materials have been introduced as potential 3D cell culture scaffolds. These include protein extracts, peptide amphiphiles, and synthetic polymers. Hydrogel scaffolds without human or animal borne components or added bioactive components are preferred from the immunological point of view. Here we demonstrate that native nanofibrillar cellulose (NFC) hydrogels derived from the abundant plant sources provide the desired functionalities. We show 1) rheological properties that allow formation of a 3D scaffold in-situ after facile injection, 2) cellular biocompatibility without added growth factors, 3) cellular polarization, and 4) differentiation of human hepatic cell lines HepaRG and HepG2. At high shear stress, the aqueous NFC has small viscosity that supports injectability, whereas at low shear stress conditions the material is converted to an elastic gel. Due to the inherent biocompatibility without any additives, we conclude that NFC generates a feasible and sustained microenvironment for 3D cell culture for potential applications, such as drug and chemical testing, tissue engineering, and cell therapy.

AB - Over the recent years, various materials have been introduced as potential 3D cell culture scaffolds. These include protein extracts, peptide amphiphiles, and synthetic polymers. Hydrogel scaffolds without human or animal borne components or added bioactive components are preferred from the immunological point of view. Here we demonstrate that native nanofibrillar cellulose (NFC) hydrogels derived from the abundant plant sources provide the desired functionalities. We show 1) rheological properties that allow formation of a 3D scaffold in-situ after facile injection, 2) cellular biocompatibility without added growth factors, 3) cellular polarization, and 4) differentiation of human hepatic cell lines HepaRG and HepG2. At high shear stress, the aqueous NFC has small viscosity that supports injectability, whereas at low shear stress conditions the material is converted to an elastic gel. Due to the inherent biocompatibility without any additives, we conclude that NFC generates a feasible and sustained microenvironment for 3D cell culture for potential applications, such as drug and chemical testing, tissue engineering, and cell therapy.

KW - 3D cell culture

KW - Hepatocyte

KW - Nanocellulose

KW - Nanofiber

KW - Plant-derived cellulose

U2 - 10.1016/j.jconrel.2012.06.039

DO - 10.1016/j.jconrel.2012.06.039

M3 - Article

VL - 164

SP - 291

EP - 298

JO - Journal of Controlled Release

JF - Journal of Controlled Release

SN - 0168-3659

IS - 3

ER -