Nanofibrous chitosan-polyethylene oxide engineered scaffolds: A comparative study between simulated structural characteristics and cells viability

M Kazemi Pilehrood, M Dilamian, M Mirian, H Sadeghi-Aliabadi, L Maleknia, P Nousiainen, Ali Harlin

Research output: Contribution to journalArticleScientificpeer-review

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Abstract

3D nanofibrous chitosan-polyethylene oxide (PEO) scaffolds were fabricated by electrospinning at different processing parameters. The structural characteristics, such as pore size, overall porosity, pore interconnectivity, and scaffold percolative efficiency (SPE), were simulated by a robust image analysis. Mouse fibroblast cells (L929) were cultured in RPMI for 2 days in the presence of various samples of nanofibrous chitosan/PEO scaffolds. Cell attachments and corresponding mean viability were enhanced from 50% to 110% compared to that belonging to a control even at packed morphologies of scaffolds constituted from pores with nanoscale diameter. To elucidate the correlation between structural characteristics within the depth of the scaffolds' profile and cell viability, a comparative analysis was proposed. This analysis revealed that larger fiber diameters and pore sizes can enhance cell viability. On the contrary, increasing the other structural elements such as overall porosity and interconnectivity due to a simultaneous reduction in fiber diameter and pore size through the electrospinning process can reduce the viability of cells. In addition, it was found that manipulation of the processing parameters in electrospinning can compensate for the effects of packed morphologies of nanofibrous scaffolds and can thus potentially improve the infiltration and viability of cells
Original languageEnglish
Article number438065
Number of pages9
JournalBioMed Research International
Volume2014
DOIs
Publication statusPublished - 2014
MoE publication typeA1 Journal article-refereed

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Chitosan
Scaffolds
Cell Survival
Cells
Porosity
Electrospinning
Pore size
Fibers
Fibroblasts
Processing
Infiltration
Image analysis

Cite this

@article{753d4e565f074ae9a227035a806e09d1,
title = "Nanofibrous chitosan-polyethylene oxide engineered scaffolds: A comparative study between simulated structural characteristics and cells viability",
abstract = "3D nanofibrous chitosan-polyethylene oxide (PEO) scaffolds were fabricated by electrospinning at different processing parameters. The structural characteristics, such as pore size, overall porosity, pore interconnectivity, and scaffold percolative efficiency (SPE), were simulated by a robust image analysis. Mouse fibroblast cells (L929) were cultured in RPMI for 2 days in the presence of various samples of nanofibrous chitosan/PEO scaffolds. Cell attachments and corresponding mean viability were enhanced from 50{\%} to 110{\%} compared to that belonging to a control even at packed morphologies of scaffolds constituted from pores with nanoscale diameter. To elucidate the correlation between structural characteristics within the depth of the scaffolds' profile and cell viability, a comparative analysis was proposed. This analysis revealed that larger fiber diameters and pore sizes can enhance cell viability. On the contrary, increasing the other structural elements such as overall porosity and interconnectivity due to a simultaneous reduction in fiber diameter and pore size through the electrospinning process can reduce the viability of cells. In addition, it was found that manipulation of the processing parameters in electrospinning can compensate for the effects of packed morphologies of nanofibrous scaffolds and can thus potentially improve the infiltration and viability of cells",
author = "{Kazemi Pilehrood}, M and M Dilamian and M Mirian and H Sadeghi-Aliabadi and L Maleknia and P Nousiainen and Ali Harlin",
year = "2014",
doi = "10.1155/2014/438065",
language = "English",
volume = "2014",
journal = "BioMed Research International",
issn = "2314-6133",
publisher = "Hindawi",

}

Nanofibrous chitosan-polyethylene oxide engineered scaffolds : A comparative study between simulated structural characteristics and cells viability. / Kazemi Pilehrood, M; Dilamian, M; Mirian, M; Sadeghi-Aliabadi, H; Maleknia, L; Nousiainen, P; Harlin, Ali.

In: BioMed Research International, Vol. 2014, 438065, 2014.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Nanofibrous chitosan-polyethylene oxide engineered scaffolds

T2 - A comparative study between simulated structural characteristics and cells viability

AU - Kazemi Pilehrood, M

AU - Dilamian, M

AU - Mirian, M

AU - Sadeghi-Aliabadi, H

AU - Maleknia, L

AU - Nousiainen, P

AU - Harlin, Ali

PY - 2014

Y1 - 2014

N2 - 3D nanofibrous chitosan-polyethylene oxide (PEO) scaffolds were fabricated by electrospinning at different processing parameters. The structural characteristics, such as pore size, overall porosity, pore interconnectivity, and scaffold percolative efficiency (SPE), were simulated by a robust image analysis. Mouse fibroblast cells (L929) were cultured in RPMI for 2 days in the presence of various samples of nanofibrous chitosan/PEO scaffolds. Cell attachments and corresponding mean viability were enhanced from 50% to 110% compared to that belonging to a control even at packed morphologies of scaffolds constituted from pores with nanoscale diameter. To elucidate the correlation between structural characteristics within the depth of the scaffolds' profile and cell viability, a comparative analysis was proposed. This analysis revealed that larger fiber diameters and pore sizes can enhance cell viability. On the contrary, increasing the other structural elements such as overall porosity and interconnectivity due to a simultaneous reduction in fiber diameter and pore size through the electrospinning process can reduce the viability of cells. In addition, it was found that manipulation of the processing parameters in electrospinning can compensate for the effects of packed morphologies of nanofibrous scaffolds and can thus potentially improve the infiltration and viability of cells

AB - 3D nanofibrous chitosan-polyethylene oxide (PEO) scaffolds were fabricated by electrospinning at different processing parameters. The structural characteristics, such as pore size, overall porosity, pore interconnectivity, and scaffold percolative efficiency (SPE), were simulated by a robust image analysis. Mouse fibroblast cells (L929) were cultured in RPMI for 2 days in the presence of various samples of nanofibrous chitosan/PEO scaffolds. Cell attachments and corresponding mean viability were enhanced from 50% to 110% compared to that belonging to a control even at packed morphologies of scaffolds constituted from pores with nanoscale diameter. To elucidate the correlation between structural characteristics within the depth of the scaffolds' profile and cell viability, a comparative analysis was proposed. This analysis revealed that larger fiber diameters and pore sizes can enhance cell viability. On the contrary, increasing the other structural elements such as overall porosity and interconnectivity due to a simultaneous reduction in fiber diameter and pore size through the electrospinning process can reduce the viability of cells. In addition, it was found that manipulation of the processing parameters in electrospinning can compensate for the effects of packed morphologies of nanofibrous scaffolds and can thus potentially improve the infiltration and viability of cells

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DO - 10.1155/2014/438065

M3 - Article

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JO - BioMed Research International

JF - BioMed Research International

SN - 2314-6133

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