Nanosized TiO2 caused minor airflow limitation in the murine airways

Maija Leppänen (Corresponding Author), Anne Korpi, Mirella Miettinen, Jani Leskinen, Tiina Torvela, Elina M. Rossi, Esa Vanhala, Henrik Wolff, Harri Alenius, Veli-Matti Kosma, Jorma Joutsensaari, Jorma Jokiniemi, Pertti Pasanen

Research output: Contribution to journalArticleScientificpeer-review

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Abstract

The use of nanotechnology is increasing exponentially, whereas the possible adverse health effects of engineered nanoparticles (NPs) are so far less known. Standardized mouse bioassay was used to study sensory and pulmonary irritation, airflow limitation, and inflammation potency of nanosized TiO2. Single exposure (0.5 h) to in situ generated TiO2 (primary particle size 20 nm; geometric mean diameters of 91, 113, and 130 nm at mass concentrations of 8, 20, and 30 mg/m3, respectively; crystal phase anatase + brookite (3:1)) caused airflow limitation in the conducting airways at each studied exposure concentration, which was shown as a reduction in expiratory flow, being at the lowest 73% of baseline. The response was not dose dependent. Repeated exposures (altogether 16 h, 1 h/day, 4 days/week for 4 weeks) to TiO2 at mass concentration of 30 mg/m3 caused as intense airflow limitation effect as the single exposures, and the extent of the responses stayed about the same along the exposure days. Sensory irritation was fairly minor. Pulmonary irritation was more pronounced during the latter part of the repeated exposures compared to the single exposures and the beginning of the repeated exposures. Sensory and pulmonary irritation were observed also in the control group, and, therefore, reaction by-products (NO2 and C3H6) may have contributed to the irritation effects. TiO2 NPs accumulated mainly in the pulmonary macrophages, and they did not cause nasal or pulmonary inflammation. In conclusion, the irritation and inflammation potencies of studied TiO2 seemed to be low.
Original languageEnglish
Pages (from-to)827-839
JournalArchives of Toxicology
Volume85
Issue number7
DOIs
Publication statusPublished - 2011
MoE publication typeA1 Journal article-refereed

Fingerprint

Nanoparticles
Lung
Bioassay
Nanotechnology
Inflammation
Byproducts
Particle size
Alveolar Macrophages
Health
Nose
Particle Size
Biological Assay
Crystals
Pneumonia
Control Groups
titanium dioxide

Keywords

  • Airflow limitation
  • engineered nanoparticles
  • inflammation
  • irritation
  • titanium dioxide

Cite this

Leppänen, M., Korpi, A., Miettinen, M., Leskinen, J., Torvela, T., Rossi, E. M., ... Pasanen, P. (2011). Nanosized TiO2 caused minor airflow limitation in the murine airways. Archives of Toxicology, 85(7), 827-839. https://doi.org/10.1007/s00204-011-0644-y
Leppänen, Maija ; Korpi, Anne ; Miettinen, Mirella ; Leskinen, Jani ; Torvela, Tiina ; Rossi, Elina M. ; Vanhala, Esa ; Wolff, Henrik ; Alenius, Harri ; Kosma, Veli-Matti ; Joutsensaari, Jorma ; Jokiniemi, Jorma ; Pasanen, Pertti. / Nanosized TiO2 caused minor airflow limitation in the murine airways. In: Archives of Toxicology. 2011 ; Vol. 85, No. 7. pp. 827-839.
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Leppänen, M, Korpi, A, Miettinen, M, Leskinen, J, Torvela, T, Rossi, EM, Vanhala, E, Wolff, H, Alenius, H, Kosma, V-M, Joutsensaari, J, Jokiniemi, J & Pasanen, P 2011, 'Nanosized TiO2 caused minor airflow limitation in the murine airways', Archives of Toxicology, vol. 85, no. 7, pp. 827-839. https://doi.org/10.1007/s00204-011-0644-y

Nanosized TiO2 caused minor airflow limitation in the murine airways. / Leppänen, Maija (Corresponding Author); Korpi, Anne; Miettinen, Mirella; Leskinen, Jani; Torvela, Tiina; Rossi, Elina M.; Vanhala, Esa; Wolff, Henrik; Alenius, Harri; Kosma, Veli-Matti; Joutsensaari, Jorma; Jokiniemi, Jorma; Pasanen, Pertti.

In: Archives of Toxicology, Vol. 85, No. 7, 2011, p. 827-839.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Nanosized TiO2 caused minor airflow limitation in the murine airways

AU - Leppänen, Maija

AU - Korpi, Anne

AU - Miettinen, Mirella

AU - Leskinen, Jani

AU - Torvela, Tiina

AU - Rossi, Elina M.

AU - Vanhala, Esa

AU - Wolff, Henrik

AU - Alenius, Harri

AU - Kosma, Veli-Matti

AU - Joutsensaari, Jorma

AU - Jokiniemi, Jorma

AU - Pasanen, Pertti

PY - 2011

Y1 - 2011

N2 - The use of nanotechnology is increasing exponentially, whereas the possible adverse health effects of engineered nanoparticles (NPs) are so far less known. Standardized mouse bioassay was used to study sensory and pulmonary irritation, airflow limitation, and inflammation potency of nanosized TiO2. Single exposure (0.5 h) to in situ generated TiO2 (primary particle size 20 nm; geometric mean diameters of 91, 113, and 130 nm at mass concentrations of 8, 20, and 30 mg/m3, respectively; crystal phase anatase + brookite (3:1)) caused airflow limitation in the conducting airways at each studied exposure concentration, which was shown as a reduction in expiratory flow, being at the lowest 73% of baseline. The response was not dose dependent. Repeated exposures (altogether 16 h, 1 h/day, 4 days/week for 4 weeks) to TiO2 at mass concentration of 30 mg/m3 caused as intense airflow limitation effect as the single exposures, and the extent of the responses stayed about the same along the exposure days. Sensory irritation was fairly minor. Pulmonary irritation was more pronounced during the latter part of the repeated exposures compared to the single exposures and the beginning of the repeated exposures. Sensory and pulmonary irritation were observed also in the control group, and, therefore, reaction by-products (NO2 and C3H6) may have contributed to the irritation effects. TiO2 NPs accumulated mainly in the pulmonary macrophages, and they did not cause nasal or pulmonary inflammation. In conclusion, the irritation and inflammation potencies of studied TiO2 seemed to be low.

AB - The use of nanotechnology is increasing exponentially, whereas the possible adverse health effects of engineered nanoparticles (NPs) are so far less known. Standardized mouse bioassay was used to study sensory and pulmonary irritation, airflow limitation, and inflammation potency of nanosized TiO2. Single exposure (0.5 h) to in situ generated TiO2 (primary particle size 20 nm; geometric mean diameters of 91, 113, and 130 nm at mass concentrations of 8, 20, and 30 mg/m3, respectively; crystal phase anatase + brookite (3:1)) caused airflow limitation in the conducting airways at each studied exposure concentration, which was shown as a reduction in expiratory flow, being at the lowest 73% of baseline. The response was not dose dependent. Repeated exposures (altogether 16 h, 1 h/day, 4 days/week for 4 weeks) to TiO2 at mass concentration of 30 mg/m3 caused as intense airflow limitation effect as the single exposures, and the extent of the responses stayed about the same along the exposure days. Sensory irritation was fairly minor. Pulmonary irritation was more pronounced during the latter part of the repeated exposures compared to the single exposures and the beginning of the repeated exposures. Sensory and pulmonary irritation were observed also in the control group, and, therefore, reaction by-products (NO2 and C3H6) may have contributed to the irritation effects. TiO2 NPs accumulated mainly in the pulmonary macrophages, and they did not cause nasal or pulmonary inflammation. In conclusion, the irritation and inflammation potencies of studied TiO2 seemed to be low.

KW - Airflow limitation

KW - engineered nanoparticles

KW - inflammation

KW - irritation

KW - titanium dioxide

U2 - 10.1007/s00204-011-0644-y

DO - 10.1007/s00204-011-0644-y

M3 - Article

VL - 85

SP - 827

EP - 839

JO - Archives of Toxicology

JF - Archives of Toxicology

SN - 0340-5761

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Leppänen M, Korpi A, Miettinen M, Leskinen J, Torvela T, Rossi EM et al. Nanosized TiO2 caused minor airflow limitation in the murine airways. Archives of Toxicology. 2011;85(7):827-839. https://doi.org/10.1007/s00204-011-0644-y