Abstract
| Original language | English |
|---|---|
| Publication status | Published - 2003 |
| Event | Oxford 2003 Scanning Probe Microscopy, Sensors and Nanostructures - Heythrop Park, United Kingdom Duration: 23 May 2003 → 26 May 2003 |
Conference
| Conference | Oxford 2003 Scanning Probe Microscopy, Sensors and Nanostructures |
|---|---|
| Country | United Kingdom |
| City | Heythrop Park |
| Period | 23/05/03 → 26/05/03 |
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Nanostructural molecular films of two class II hydrophobins. / Paananen, Arja; Vuorimaa, Elina; Torkkeli, Mika; Penttilä, Merja; Ikkala, Olli; Lemmetyinen, Helge; Serimaa, Ritva; Linder, Markus.
2003. Paper presented at Oxford 2003 Scanning Probe Microscopy, Sensors and Nanostructures, Heythrop Park, United Kingdom.Research output: Contribution to conference › Conference article › Scientific
TY - CONF
T1 - Nanostructural molecular films of two class II hydrophobins
AU - Paananen, Arja
AU - Vuorimaa, Elina
AU - Torkkeli, Mika
AU - Penttilä, Merja
AU - Ikkala, Olli
AU - Lemmetyinen, Helge
AU - Serimaa, Ritva
AU - Linder, Markus
N1 - CA2: BEL1 CA2: BEL2 CA: BEL PGN: 1 p.
PY - 2003
Y1 - 2003
N2 - Hydrophobins are a group of highly surface active, self-assembling fungal proteins. They function as coatings on various fungal structures, work as adhesive agents, and enable aerial growth of hyphae. In this work we have focussed on two class II hydrophobins from Trichoderma reesei, HFBI and HFBII [1]. Langmuir-Blodgett films of HFBI and HFBII analysed by atomic force microscopy (AFM) showed highly organised two-dimensional crystalline structures for both hydrophobins [2]. Comparison between AFM data of ordered films and small-angle X-ray scattering results of hydrophobin solutions [3] indicated that the unit cells in the films have dimensions similar to those of tetrameric aggregates found in solutions. The results also indicated functional and structural differences between HFBI and HFBII that help to explain differences in their properties. This detailed structural study of hydrophobin films brings the interest in new options for surface modification and nanostructured materials. [1] Linder, M., Selber, K., Nakari-Setälä, T., Qiao, M., Kula, M.-R., and Penttilä, M., The hydrophobins HFBI and HFBII from Trichoderma reesei showing efficient interactions with nonionic surfactants in aqueous two-phase systems, Biomacromolecules 2 (2001) 511-517. [2] Paananen, A., Vuorimaa, E., Torkkeli, M., Penttilä, M., Kauranen, M., Ikkala, O., Lemmetyinen, H., Serimaa, R., and Linder, M., Structural hierarchy in molecular films of two class II hydrophobins, Biochemistry 42 (2003) 5253-5258. [3] Torkkeli, M., Serimaa, R., Ikkala, O., and Linder, M., Aggregation and self-assembly of hydrophobins from Trichoderma reesei: Low-resolution structural models, Biophysical Journal 83 (2002) 2240-2247.
AB - Hydrophobins are a group of highly surface active, self-assembling fungal proteins. They function as coatings on various fungal structures, work as adhesive agents, and enable aerial growth of hyphae. In this work we have focussed on two class II hydrophobins from Trichoderma reesei, HFBI and HFBII [1]. Langmuir-Blodgett films of HFBI and HFBII analysed by atomic force microscopy (AFM) showed highly organised two-dimensional crystalline structures for both hydrophobins [2]. Comparison between AFM data of ordered films and small-angle X-ray scattering results of hydrophobin solutions [3] indicated that the unit cells in the films have dimensions similar to those of tetrameric aggregates found in solutions. The results also indicated functional and structural differences between HFBI and HFBII that help to explain differences in their properties. This detailed structural study of hydrophobin films brings the interest in new options for surface modification and nanostructured materials. [1] Linder, M., Selber, K., Nakari-Setälä, T., Qiao, M., Kula, M.-R., and Penttilä, M., The hydrophobins HFBI and HFBII from Trichoderma reesei showing efficient interactions with nonionic surfactants in aqueous two-phase systems, Biomacromolecules 2 (2001) 511-517. [2] Paananen, A., Vuorimaa, E., Torkkeli, M., Penttilä, M., Kauranen, M., Ikkala, O., Lemmetyinen, H., Serimaa, R., and Linder, M., Structural hierarchy in molecular films of two class II hydrophobins, Biochemistry 42 (2003) 5253-5258. [3] Torkkeli, M., Serimaa, R., Ikkala, O., and Linder, M., Aggregation and self-assembly of hydrophobins from Trichoderma reesei: Low-resolution structural models, Biophysical Journal 83 (2002) 2240-2247.
M3 - Conference article
ER -