Nanostructural molecular films of two class II hydrophobins

Arja Paananen, Elina Vuorimaa, Mika Torkkeli, Merja Penttilä, Olli Ikkala, Helge Lemmetyinen, Ritva Serimaa, Markus Linder

Research output: Contribution to conferenceConference articleScientific

Abstract

Hydrophobins are a group of highly surface active, self-assembling fungal proteins. They function as coatings on various fungal structures, work as adhesive agents, and enable aerial growth of hyphae. In this work we have focussed on two class II hydrophobins from Trichoderma reesei, HFBI and HFBII [1]. Langmuir-Blodgett films of HFBI and HFBII analysed by atomic force microscopy (AFM) showed highly organised two-dimensional crystalline structures for both hydrophobins [2]. Comparison between AFM data of ordered films and small-angle X-ray scattering results of hydrophobin solutions [3] indicated that the unit cells in the films have dimensions similar to those of tetrameric aggregates found in solutions. The results also indicated functional and structural differences between HFBI and HFBII that help to explain differences in their properties. This detailed structural study of hydrophobin films brings the interest in new options for surface modification and nanostructured materials. [1] Linder, M., Selber, K., Nakari-Setälä, T., Qiao, M., Kula, M.-R., and Penttilä, M., The hydrophobins HFBI and HFBII from Trichoderma reesei showing efficient interactions with nonionic surfactants in aqueous two-phase systems, Biomacromolecules 2 (2001) 511-517. [2] Paananen, A., Vuorimaa, E., Torkkeli, M., Penttilä, M., Kauranen, M., Ikkala, O., Lemmetyinen, H., Serimaa, R., and Linder, M., Structural hierarchy in molecular films of two class II hydrophobins, Biochemistry 42 (2003) 5253-5258. [3] Torkkeli, M., Serimaa, R., Ikkala, O., and Linder, M., Aggregation and self-assembly of hydrophobins from Trichoderma reesei: Low-resolution structural models, Biophysical Journal 83 (2002) 2240-2247.
Original languageEnglish
Publication statusPublished - 2003
EventOxford 2003 Scanning Probe Microscopy, Sensors and Nanostructures - Heythrop Park, United Kingdom
Duration: 23 May 200326 May 2003

Conference

ConferenceOxford 2003 Scanning Probe Microscopy, Sensors and Nanostructures
CountryUnited Kingdom
CityHeythrop Park
Period23/05/0326/05/03

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Atomic force microscopy
Biochemistry
Fungal Proteins
Langmuir Blodgett films
Nonionic surfactants
X ray scattering
Nanostructured materials
Self assembly
Surface treatment
Adhesives
Agglomeration
Antennas
Crystalline materials
Coatings
1-(heptafluorobutyryl)imidazole

Cite this

Paananen, A., Vuorimaa, E., Torkkeli, M., Penttilä, M., Ikkala, O., Lemmetyinen, H., ... Linder, M. (2003). Nanostructural molecular films of two class II hydrophobins. Paper presented at Oxford 2003 Scanning Probe Microscopy, Sensors and Nanostructures, Heythrop Park, United Kingdom.
Paananen, Arja ; Vuorimaa, Elina ; Torkkeli, Mika ; Penttilä, Merja ; Ikkala, Olli ; Lemmetyinen, Helge ; Serimaa, Ritva ; Linder, Markus. / Nanostructural molecular films of two class II hydrophobins. Paper presented at Oxford 2003 Scanning Probe Microscopy, Sensors and Nanostructures, Heythrop Park, United Kingdom.
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Paananen, A, Vuorimaa, E, Torkkeli, M, Penttilä, M, Ikkala, O, Lemmetyinen, H, Serimaa, R & Linder, M 2003, 'Nanostructural molecular films of two class II hydrophobins' Paper presented at Oxford 2003 Scanning Probe Microscopy, Sensors and Nanostructures, Heythrop Park, United Kingdom, 23/05/03 - 26/05/03, .

Nanostructural molecular films of two class II hydrophobins. / Paananen, Arja; Vuorimaa, Elina; Torkkeli, Mika; Penttilä, Merja; Ikkala, Olli; Lemmetyinen, Helge; Serimaa, Ritva; Linder, Markus.

2003. Paper presented at Oxford 2003 Scanning Probe Microscopy, Sensors and Nanostructures, Heythrop Park, United Kingdom.

Research output: Contribution to conferenceConference articleScientific

TY - CONF

T1 - Nanostructural molecular films of two class II hydrophobins

AU - Paananen, Arja

AU - Vuorimaa, Elina

AU - Torkkeli, Mika

AU - Penttilä, Merja

AU - Ikkala, Olli

AU - Lemmetyinen, Helge

AU - Serimaa, Ritva

AU - Linder, Markus

N1 - CA2: BEL1 CA2: BEL2 CA: BEL PGN: 1 p.

PY - 2003

Y1 - 2003

N2 - Hydrophobins are a group of highly surface active, self-assembling fungal proteins. They function as coatings on various fungal structures, work as adhesive agents, and enable aerial growth of hyphae. In this work we have focussed on two class II hydrophobins from Trichoderma reesei, HFBI and HFBII [1]. Langmuir-Blodgett films of HFBI and HFBII analysed by atomic force microscopy (AFM) showed highly organised two-dimensional crystalline structures for both hydrophobins [2]. Comparison between AFM data of ordered films and small-angle X-ray scattering results of hydrophobin solutions [3] indicated that the unit cells in the films have dimensions similar to those of tetrameric aggregates found in solutions. The results also indicated functional and structural differences between HFBI and HFBII that help to explain differences in their properties. This detailed structural study of hydrophobin films brings the interest in new options for surface modification and nanostructured materials. [1] Linder, M., Selber, K., Nakari-Setälä, T., Qiao, M., Kula, M.-R., and Penttilä, M., The hydrophobins HFBI and HFBII from Trichoderma reesei showing efficient interactions with nonionic surfactants in aqueous two-phase systems, Biomacromolecules 2 (2001) 511-517. [2] Paananen, A., Vuorimaa, E., Torkkeli, M., Penttilä, M., Kauranen, M., Ikkala, O., Lemmetyinen, H., Serimaa, R., and Linder, M., Structural hierarchy in molecular films of two class II hydrophobins, Biochemistry 42 (2003) 5253-5258. [3] Torkkeli, M., Serimaa, R., Ikkala, O., and Linder, M., Aggregation and self-assembly of hydrophobins from Trichoderma reesei: Low-resolution structural models, Biophysical Journal 83 (2002) 2240-2247.

AB - Hydrophobins are a group of highly surface active, self-assembling fungal proteins. They function as coatings on various fungal structures, work as adhesive agents, and enable aerial growth of hyphae. In this work we have focussed on two class II hydrophobins from Trichoderma reesei, HFBI and HFBII [1]. Langmuir-Blodgett films of HFBI and HFBII analysed by atomic force microscopy (AFM) showed highly organised two-dimensional crystalline structures for both hydrophobins [2]. Comparison between AFM data of ordered films and small-angle X-ray scattering results of hydrophobin solutions [3] indicated that the unit cells in the films have dimensions similar to those of tetrameric aggregates found in solutions. The results also indicated functional and structural differences between HFBI and HFBII that help to explain differences in their properties. This detailed structural study of hydrophobin films brings the interest in new options for surface modification and nanostructured materials. [1] Linder, M., Selber, K., Nakari-Setälä, T., Qiao, M., Kula, M.-R., and Penttilä, M., The hydrophobins HFBI and HFBII from Trichoderma reesei showing efficient interactions with nonionic surfactants in aqueous two-phase systems, Biomacromolecules 2 (2001) 511-517. [2] Paananen, A., Vuorimaa, E., Torkkeli, M., Penttilä, M., Kauranen, M., Ikkala, O., Lemmetyinen, H., Serimaa, R., and Linder, M., Structural hierarchy in molecular films of two class II hydrophobins, Biochemistry 42 (2003) 5253-5258. [3] Torkkeli, M., Serimaa, R., Ikkala, O., and Linder, M., Aggregation and self-assembly of hydrophobins from Trichoderma reesei: Low-resolution structural models, Biophysical Journal 83 (2002) 2240-2247.

M3 - Conference article

ER -

Paananen A, Vuorimaa E, Torkkeli M, Penttilä M, Ikkala O, Lemmetyinen H et al. Nanostructural molecular films of two class II hydrophobins. 2003. Paper presented at Oxford 2003 Scanning Probe Microscopy, Sensors and Nanostructures, Heythrop Park, United Kingdom.