TY - JOUR
T1 - Natural Antimicrobials from Cloudberry (Rubus chamaemorus) Seeds by Sanding and Hydrothermal Extraction
AU - Puupponen-pimiä, Riitta
AU - Nohynek, Liisa
AU - Suvanto, Jussi
AU - Salminen, Juha-pekka
AU - Seppänen-laakso, Tuulikki
AU - Tähtiharju, Juha
AU - Honkapää, Kaisu
AU - Oksman-caldentey, Kirsi-marja
N1 - Funding
Business Finland - the Finnish Funding Agency for Technology and Innovation (Commercialization of Berry-based Natural Ingredients in Cosmetics, project (No. 703/31/2015) during the years 2015–2017) is acknowledged for financial support. The study was also financed by VTT Technical Research Centre of Finland Ltd, iBEX Innovative Business to solve Exponential problems Program, Natural Compounds against antibiotic resistance project during the year 2018.
PY - 2021/6/18
Y1 - 2021/6/18
N2 - We have developed an organic solvent-free process to enrich natural antimicrobials from the important Nordic Rubus berry species, especially from cloudberry. The process utilizes industrial berry byproducts as raw-material, and it is based on seed sanding technology and water-based extraction. The extracts showed strong antimicrobial activity against Gram-positive Staphylococcus aureus and methicillin-resistant Staphylococcus aureus (MRSA). A concentration of 5 mg/mL totally eliminated these bacteria in 24 h. Effects against Gram-negative bacteria were modest. Polyphenol analysis of the hydrothermal extracts showed that the antimicrobial activity is correlated to ellagitannins, mainly dimeric sanguiin H-6 and sanguiin H-10 isomers. Sanguiin H-10 is not commonly found in intact cloudberry seeds, but it is formed from the dimeric and trimeric ellagitannins, during the extraction. Surprisingly hydrothermal extracts had no or minor effects on beneficial bacteria, Lactobacillus rhamnosus. This interesting finding might offer an application potential in controlling skin microbial pathogens, for example, in wound healing.
AB - We have developed an organic solvent-free process to enrich natural antimicrobials from the important Nordic Rubus berry species, especially from cloudberry. The process utilizes industrial berry byproducts as raw-material, and it is based on seed sanding technology and water-based extraction. The extracts showed strong antimicrobial activity against Gram-positive Staphylococcus aureus and methicillin-resistant Staphylococcus aureus (MRSA). A concentration of 5 mg/mL totally eliminated these bacteria in 24 h. Effects against Gram-negative bacteria were modest. Polyphenol analysis of the hydrothermal extracts showed that the antimicrobial activity is correlated to ellagitannins, mainly dimeric sanguiin H-6 and sanguiin H-10 isomers. Sanguiin H-10 is not commonly found in intact cloudberry seeds, but it is formed from the dimeric and trimeric ellagitannins, during the extraction. Surprisingly hydrothermal extracts had no or minor effects on beneficial bacteria, Lactobacillus rhamnosus. This interesting finding might offer an application potential in controlling skin microbial pathogens, for example, in wound healing.
KW - Rubus species
KW - berries
KW - byproducts
KW - seeds
KW - antimicrobial activity
KW - MRSA
UR - http://www.scopus.com/inward/record.url?scp=85120069645&partnerID=8YFLogxK
U2 - 10.1021/acsfoodscitech.0c00109
DO - 10.1021/acsfoodscitech.0c00109
M3 - Article
SN - 2692-1944
VL - 1
SP - 917
EP - 927
JO - ACS Food Science & Technology
JF - ACS Food Science & Technology
IS - 5
ER -
