Natural Antimicrobials from Cloudberry (Rubus chamaemorus) Seeds by Sanding and Hydrothermal Extraction

Riitta Puupponen-pimiä; Liisa Nohynek; Jussi Suvanto; Juha-pekka Salminen; Tuulikki Seppänen-laakso; Juha Tähtiharju; Kaisu Honkapää; Kirsi-marja Oksman-caldentey

doi:10.1021/acsfoodscitech.0c00109

Natural Antimicrobials from Cloudberry (Rubus chamaemorus) Seeds by Sanding and Hydrothermal Extraction

Riitta Puupponen-pimiä^*
, Liisa Nohynek
, Jussi Suvanto
, Juha-pekka Salminen
, Tuulikki Seppänen-laakso
, Juha Tähtiharju
, Kaisu Honkapää
, Kirsi-marja Oksman-caldentey

^*Corresponding author for this work

University of Turku

Research output: Contribution to journal › Article › Scientific › peer-review

18 Citations (Scopus)

432 Downloads (Pure)

Abstract

We have developed an organic solvent-free process to enrich natural antimicrobials from the important Nordic Rubus berry species, especially from cloudberry. The process utilizes industrial berry byproducts as raw-material, and it is based on seed sanding technology and water-based extraction. The extracts showed strong antimicrobial activity against Gram-positive Staphylococcus aureus and methicillin-resistant Staphylococcus aureus (MRSA). A concentration of 5 mg/mL totally eliminated these bacteria in 24 h. Effects against Gram-negative bacteria were modest. Polyphenol analysis of the hydrothermal extracts showed that the antimicrobial activity is correlated to ellagitannins, mainly dimeric sanguiin H-6 and sanguiin H-10 isomers. Sanguiin H-10 is not commonly found in intact cloudberry seeds, but it is formed from the dimeric and trimeric ellagitannins, during the extraction. Surprisingly hydrothermal extracts had no or minor effects on beneficial bacteria, Lactobacillus rhamnosus. This interesting finding might offer an application potential in controlling skin microbial pathogens, for example, in wound healing.

Original language	English
Pages (from-to)	917-927
Journal	ACS Food Science & Technology
Volume	1
Issue number	5
DOIs	https://doi.org/10.1021/acsfoodscitech.0c00109
Publication status	Published - 18 Jun 2021
MoE publication type	A1 Journal article-refereed

Funding

Business Finland - the Finnish Funding Agency for Technology and Innovation (Commercialization of Berry-based Natural Ingredients in Cosmetics, project (No. 703/31/2015) during the years 2015–2017) is acknowledged for financial support. The study was also financed by VTT Technical Research Centre of Finland Ltd, iBEX Innovative Business to solve Exponential problems Program, Natural Compounds against antibiotic resistance project during the year 2018.

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Rubus species
berries
byproducts
seeds
antimicrobial activity
MRSA

Access to Document

10.1021/acsfoodscitech.0c00109Licence: CC BY

acsfoodscitech.0c00109Final published version, 5.64 MBLicence: CC BY

Cite this

@article{b31143be461d419fa23470a7e8eb83cb,

title = "Natural Antimicrobials from Cloudberry (Rubus chamaemorus) Seeds by Sanding and Hydrothermal Extraction",

abstract = "We have developed an organic solvent-free process to enrich natural antimicrobials from the important Nordic Rubus berry species, especially from cloudberry. The process utilizes industrial berry byproducts as raw-material, and it is based on seed sanding technology and water-based extraction. The extracts showed strong antimicrobial activity against Gram-positive Staphylococcus aureus and methicillin-resistant Staphylococcus aureus (MRSA). A concentration of 5 mg/mL totally eliminated these bacteria in 24 h. Effects against Gram-negative bacteria were modest. Polyphenol analysis of the hydrothermal extracts showed that the antimicrobial activity is correlated to ellagitannins, mainly dimeric sanguiin H-6 and sanguiin H-10 isomers. Sanguiin H-10 is not commonly found in intact cloudberry seeds, but it is formed from the dimeric and trimeric ellagitannins, during the extraction. Surprisingly hydrothermal extracts had no or minor effects on beneficial bacteria, Lactobacillus rhamnosus. This interesting finding might offer an application potential in controlling skin microbial pathogens, for example, in wound healing.",

keywords = "Rubus species, berries, byproducts, seeds, antimicrobial activity, MRSA",

author = "Riitta Puupponen-pimi{\"a} and Liisa Nohynek and Jussi Suvanto and Juha-pekka Salminen and Tuulikki Sepp{\"a}nen-laakso and Juha T{\"a}htiharju and Kaisu Honkap{\"a}{\"a} and Kirsi-marja Oksman-caldentey",

year = "2021",

month = jun,

day = "18",

doi = "10.1021/acsfoodscitech.0c00109",

language = "English",

volume = "1",

pages = "917--927",

journal = "ACS Food Science \& Technology",

issn = "2692-1944",

publisher = "American Chemical Society ACS",

number = "5",

}

TY - JOUR

T1 - Natural Antimicrobials from Cloudberry (Rubus chamaemorus) Seeds by Sanding and Hydrothermal Extraction

AU - Puupponen-pimiä, Riitta

AU - Nohynek, Liisa

AU - Suvanto, Jussi

AU - Salminen, Juha-pekka

AU - Seppänen-laakso, Tuulikki

AU - Tähtiharju, Juha

AU - Honkapää, Kaisu

AU - Oksman-caldentey, Kirsi-marja

PY - 2021/6/18

Y1 - 2021/6/18

N2 - We have developed an organic solvent-free process to enrich natural antimicrobials from the important Nordic Rubus berry species, especially from cloudberry. The process utilizes industrial berry byproducts as raw-material, and it is based on seed sanding technology and water-based extraction. The extracts showed strong antimicrobial activity against Gram-positive Staphylococcus aureus and methicillin-resistant Staphylococcus aureus (MRSA). A concentration of 5 mg/mL totally eliminated these bacteria in 24 h. Effects against Gram-negative bacteria were modest. Polyphenol analysis of the hydrothermal extracts showed that the antimicrobial activity is correlated to ellagitannins, mainly dimeric sanguiin H-6 and sanguiin H-10 isomers. Sanguiin H-10 is not commonly found in intact cloudberry seeds, but it is formed from the dimeric and trimeric ellagitannins, during the extraction. Surprisingly hydrothermal extracts had no or minor effects on beneficial bacteria, Lactobacillus rhamnosus. This interesting finding might offer an application potential in controlling skin microbial pathogens, for example, in wound healing.

AB - We have developed an organic solvent-free process to enrich natural antimicrobials from the important Nordic Rubus berry species, especially from cloudberry. The process utilizes industrial berry byproducts as raw-material, and it is based on seed sanding technology and water-based extraction. The extracts showed strong antimicrobial activity against Gram-positive Staphylococcus aureus and methicillin-resistant Staphylococcus aureus (MRSA). A concentration of 5 mg/mL totally eliminated these bacteria in 24 h. Effects against Gram-negative bacteria were modest. Polyphenol analysis of the hydrothermal extracts showed that the antimicrobial activity is correlated to ellagitannins, mainly dimeric sanguiin H-6 and sanguiin H-10 isomers. Sanguiin H-10 is not commonly found in intact cloudberry seeds, but it is formed from the dimeric and trimeric ellagitannins, during the extraction. Surprisingly hydrothermal extracts had no or minor effects on beneficial bacteria, Lactobacillus rhamnosus. This interesting finding might offer an application potential in controlling skin microbial pathogens, for example, in wound healing.

KW - Rubus species

KW - berries

KW - byproducts

KW - seeds

KW - antimicrobial activity

KW - MRSA

UR - https://www.scopus.com/pages/publications/85120069645

U2 - 10.1021/acsfoodscitech.0c00109

DO - 10.1021/acsfoodscitech.0c00109

M3 - Article

SN - 2692-1944

VL - 1

SP - 917

EP - 927

JO - ACS Food Science & Technology

JF - ACS Food Science & Technology

IS - 5

ER -

Natural Antimicrobials from Cloudberry (Rubus chamaemorus) Seeds by Sanding and Hydrothermal Extraction

Abstract

Funding

UN SDGs

Keywords

Access to Document

Other files and links

Fingerprint

Cite this