New capillary optical fiber structure for fluorescence sensors

Pauli Kiiveri, Ari Hokkanen, Reijo Kylmänen, Kari Keinänen, Simo Tammela

    Research output: Chapter in Book/Report/Conference proceedingConference article in proceedingsScientificpeer-review

    4 Citations (Scopus)


    A multilayer capillary fiber was designed for optical sensor applications and its optical properties were evaluated by using a fluorescent layer immobilized on its inner surface. This fiber structure combines a large interaction surface (the inner wall of the capillary fiber) for binding of fluorescent indicators with evanescent wave fluorescence measurement, which may facilitate the design of pseudohomogeneous assays without separation of the bound from nonbound fluorescent indicator. The inner surface of the capillary was derivatized by aminosilanization, followed by biotinylation and addition of streptavidin. This biotin- streptavidin coating facilities subsequent immobilization of any biotinylated species (e.g. antibodies, antigens, etc.) participating in specific molecular recognition. We have evaluated some properties of this capillary fiber design by using fluorescent proteins immobilized on the inner wall of capillary by biotin-avidin-interaction. Fluorescence was excited by a HeNe-laser (fluorescent indicator APC; λem = 660 nm) and by a Ar-laser (fluorescent indicator RPE, λem = 578 nm), and measured with a spectrum analyzer.
    Original languageEnglish
    Title of host publicationFunctional Photonic and Fiber Devices
    EditorsS. Iraj Najafi, Mario Nicola Armenise
    Place of PublicationBellingham
    PublisherInternational Society for Optics and Photonics SPIE
    ISBN (Print)978-0-8194-2069-5
    Publication statusPublished - 1996
    MoE publication typeA4 Article in a conference publication
    EventFunctional Photonic and Fiber Devices - San Jose, United States
    Duration: 30 Jan 19961 Feb 1996

    Publication series

    SeriesProceedings of SPIE


    ConferenceFunctional Photonic and Fiber Devices
    Country/TerritoryUnited States
    CitySan Jose


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