@inproceedings{e340b0e2554945f9a466a8e3f5555e4f,
title = "New capillary optical fiber structure for fluorescence sensors",
abstract = "A multilayer capillary fiber was designed for optical sensor applications and its optical properties were evaluated by using a fluorescent layer immobilized on its inner surface. This fiber structure combines a large interaction surface (the inner wall of the capillary fiber) for binding of fluorescent indicators with evanescent wave fluorescence measurement, which may facilitate the design of pseudohomogeneous assays without separation of the bound from nonbound fluorescent indicator. The inner surface of the capillary was derivatized by aminosilanization, followed by biotinylation and addition of streptavidin. This biotin- streptavidin coating facilities subsequent immobilization of any biotinylated species (e.g. antibodies, antigens, etc.) participating in specific molecular recognition. We have evaluated some properties of this capillary fiber design by using fluorescent proteins immobilized on the inner wall of capillary by biotin-avidin-interaction. Fluorescence was excited by a HeNe-laser (fluorescent indicator APC; λem = 660 nm) and by a Ar-laser (fluorescent indicator RPE, λem = 578 nm), and measured with a spectrum analyzer.",
author = "Pauli Kiiveri and Ari Hokkanen and Reijo Kylm{\"a}nen and Kari Kein{\"a}nen and Simo Tammela",
year = "1996",
doi = "10.1117/12.229944",
language = "English",
isbn = "978-0-8194-2069-5",
series = "Proceedings of SPIE",
publisher = "International Society for Optics and Photonics SPIE",
pages = "169--179",
editor = "Najafi, {S. Iraj} and Armenise, {Mario Nicola}",
booktitle = "Functional Photonic and Fiber Devices",
address = "United States",
note = "Functional Photonic and Fiber Devices ; Conference date: 30-01-1996 Through 01-02-1996",
}