New lager yeast strains generated by interspecific hybridization

Kristoffer Krogerus (Corresponding Author), Frederico Magalhães, Virve Vidgren, Brian Gibson

Research output: Contribution to journalArticleScientificpeer-review

54 Citations (Scopus)

Abstract

The interspecific hybrid Saccharomyces pastorianus is the most commonly used yeast in brewery fermentations worldwide. Here, we generated de novo lager yeast hybrids by mating a domesticated and strongly flocculent Saccharomyces cerevisiae ale strain with the Saccharomyces eubayanus type strain. The hybrids were characterized with respect to the parent strains in a wort fermentation performed at temperatures typical for lager brewing (12°C). The resulting beers were analysed for sugar and aroma compounds, while the yeasts were tested for their flocculation ability and [alpha]-glucoside transport capability. These hybrids inherited beneficial properties from both parent strains (cryotolerance, maltotriose utilization and strong flocculation) and showed apparent hybrid vigour, fermenting faster and producing beer with higher alcohol content (5.6 vs 4.5% ABV) than the parents. Results suggest that interspecific hybridization is suitable for production of novel non-GM lager yeast strains with unique properties and will help in elucidating the evolutionary history of industrial lager yeast.
Original languageEnglish
Pages (from-to)769-778
JournalJournal of industrial microbiology and biotechnology
Volume42
Issue number5
DOIs
Publication statusPublished - 2015
MoE publication typeA1 Journal article-refereed

Keywords

  • brewing
  • heterosis
  • lager yeasts
  • mating
  • Saccharomyces eubayanus

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