New probe immobilizations by lipoate-diethalonamines or ethylene-glycol molecules for capacitance DNA chip

Sandro Carrara, Andrea Cavallini, Yusuf Leblebici, Giovanni de Micheli, Vijayender Bhalla, Francesco Valle, Bruno Samori, Luca Benini, Bruno Ricco, Inger Vikholm-Lundin, Tony Munter

Research output: Chapter in Book/Report/Conference proceedingConference article in proceedingsScientificpeer-review

7 Citations (Scopus)

Abstract

Label-free DNA detection is of crucial role to when developing point-of-care biochips to be used in personalized therapy. Capacitance detection is a promising technology for label-free DNA detection. However, data published in literature often show evident time drift, large standard deviation, scattered data points, and poor reproducibility. To solve these problems, alkanethiol molecules such as mercapto-hexanol are usually considered as blocking agents. The aim of the present paper is to investigate new blocking agents to further improve DNA probe surfaces. Data from AFM, SPR, florescence microscopy, and capacitance measurements are used to demonstrate the new lipoates molecules. Moreover precursor layers obtained by using Ethylene-glycol alkanethiols offer further improvements in terms of diminished detection errors. Film structure is investigated at the nano-scale to justify the detection improvements in terms of probe surface quality. This study demonstrates the superiority of lipoate and Ethylene-glycol molecules as blocking candidates when immobilizing molecular probes onto spot surfaces in labelfree DNA biochip.
Original languageEnglish
Title of host publication3rd International Workshop on Advances in Sensors and Interfaces, IWASI 2009
PublisherIEEE Institute of Electrical and Electronic Engineers
Pages9-14
ISBN (Electronic)978-1-4244-4709-1
ISBN (Print)978-1-4244-4708-4
DOIs
Publication statusPublished - 2009
MoE publication typeA4 Article in a conference publication
Event3rd International Workshop on Advances in Sensors and Interfaces, IWASI 2009 - Trani, Italy
Duration: 25 Jun 200926 Jun 2009

Conference

Conference3rd International Workshop on Advances in Sensors and Interfaces, IWASI 2009
Country/TerritoryItaly
CityTrani
Period25/06/0926/06/09

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