Abstract
Label-free DNA detection is of crucial role to when
developing point-of-care biochips to be used in
personalized therapy. Capacitance detection is a
promising technology for label-free DNA detection.
However, data published in literature often show evident
time drift, large standard deviation, scattered data
points, and poor reproducibility. To solve these
problems, alkanethiol molecules such as mercapto-hexanol
are usually considered as blocking agents. The aim of the
present paper is to investigate new blocking agents to
further improve DNA probe surfaces. Data from AFM, SPR,
florescence microscopy, and capacitance measurements are
used to demonstrate the new lipoates molecules. Moreover
precursor layers obtained by using Ethylene-glycol
alkanethiols offer further improvements in terms of
diminished detection errors. Film structure is
investigated at the nano-scale to justify the detection
improvements in terms of probe surface quality. This
study demonstrates the superiority of lipoate and
Ethylene-glycol molecules as blocking candidates when
immobilizing molecular probes onto spot surfaces in
labelfree DNA biochip.
Original language | English |
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Title of host publication | 3rd International Workshop on Advances in Sensors and Interfaces, IWASI 2009 |
Publisher | IEEE Institute of Electrical and Electronic Engineers |
Pages | 9-14 |
ISBN (Electronic) | 978-1-4244-4709-1 |
ISBN (Print) | 978-1-4244-4708-4 |
DOIs | |
Publication status | Published - 2009 |
MoE publication type | A4 Article in a conference publication |
Event | 3rd International Workshop on Advances in Sensors and Interfaces, IWASI 2009 - Trani, Italy Duration: 25 Jun 2009 → 26 Jun 2009 |
Conference
Conference | 3rd International Workshop on Advances in Sensors and Interfaces, IWASI 2009 |
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Country/Territory | Italy |
City | Trani |
Period | 25/06/09 → 26/06/09 |