New types of antimicrobial compounds produced by Lactobacillus plantarum

Marja-Leena Niku-Paavola (Corresponding Author), Arja Laitila, Tiina Mattila-Sandholm, Auli Haikara

Research output: Contribution to journalArticleScientificpeer-review

238 Citations (Scopus)

Abstract

New types of antimicrobial compounds were identified in the culture filtrate of Lactobacillus plantarum VTT E‐78076. Activity was detected in the low molecular mass fraction separated by gel chromatography. This fraction totally inhibited the growth of the Gram‐negative test organism, Pantoea agglomerans (Enterobacter agglomerans) VTT E‐90396. Characteristic compounds from this fraction were identified by GC/MS‐analysis and the identification was confirmed using pure commercial reference compounds in identical chromatographs and in antimicrobial tests. The active fraction included benzoic acid (CAS 65–85–0), 5‐methyl‐2,4‐imidazolidinedione (CAS 616–03–5, methylhydantoin), tetrahydro‐4‐hydroxy‐4‐methyl‐2H‐pyran‐2‐one (CAS 674–26–0, mevalonolactone) and 3‐(2‐methylpropyl)‐2,5‐piperazinedione (CAS 5845–67–0, cyclo(glycyl‐l‐leucyl)). These compounds in concentrations of 10 ppm inhibited growth of the test organism by 10–15% when acting separately, but 100% when all were applied together with 1% lactic acid. The inhibition was 40% by 1% lactic acid alone. The compounds were also active against Fusarium avenaceum (Gibberella avenacea) VTT D‐80147. The inhibition was 10–15% by separate compounds in concentrations of 10 ppm and maximally 20% in combinations. Fungal growth was not inhibited by lactic acid. Inhibition by unfractionated Lact. plantarum culture filtrate was 37% and by the low molecular mass fraction, 27%.
Original languageEnglish
Pages (from-to)29-35
Number of pages7
JournalJournal of Applied Microbiology
Volume86
Issue number1
DOIs
Publication statusPublished - 1999
MoE publication typeA1 Journal article-refereed

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Lactobacillus plantarum
Lactic Acid
Growth
Gibberella
Pantoea
Enterobacter
Benzoic Acid
Fusarium
Gel Chromatography

Cite this

Niku-Paavola, Marja-Leena ; Laitila, Arja ; Mattila-Sandholm, Tiina ; Haikara, Auli. / New types of antimicrobial compounds produced by Lactobacillus plantarum. In: Journal of Applied Microbiology. 1999 ; Vol. 86, No. 1. pp. 29-35.
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title = "New types of antimicrobial compounds produced by Lactobacillus plantarum",
abstract = "New types of antimicrobial compounds were identified in the culture filtrate of Lactobacillus plantarum VTT E‐78076. Activity was detected in the low molecular mass fraction separated by gel chromatography. This fraction totally inhibited the growth of the Gram‐negative test organism, Pantoea agglomerans (Enterobacter agglomerans) VTT E‐90396. Characteristic compounds from this fraction were identified by GC/MS‐analysis and the identification was confirmed using pure commercial reference compounds in identical chromatographs and in antimicrobial tests. The active fraction included benzoic acid (CAS 65–85–0), 5‐methyl‐2,4‐imidazolidinedione (CAS 616–03–5, methylhydantoin), tetrahydro‐4‐hydroxy‐4‐methyl‐2H‐pyran‐2‐one (CAS 674–26–0, mevalonolactone) and 3‐(2‐methylpropyl)‐2,5‐piperazinedione (CAS 5845–67–0, cyclo(glycyl‐l‐leucyl)). These compounds in concentrations of 10 ppm inhibited growth of the test organism by 10–15{\%} when acting separately, but 100{\%} when all were applied together with 1{\%} lactic acid. The inhibition was 40{\%} by 1{\%} lactic acid alone. The compounds were also active against Fusarium avenaceum (Gibberella avenacea) VTT D‐80147. The inhibition was 10–15{\%} by separate compounds in concentrations of 10 ppm and maximally 20{\%} in combinations. Fungal growth was not inhibited by lactic acid. Inhibition by unfractionated Lact. plantarum culture filtrate was 37{\%} and by the low molecular mass fraction, 27{\%}.",
author = "Marja-Leena Niku-Paavola and Arja Laitila and Tiina Mattila-Sandholm and Auli Haikara",
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volume = "86",
pages = "29--35",
journal = "Journal of Applied Microbiology",
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Niku-Paavola, M-L, Laitila, A, Mattila-Sandholm, T & Haikara, A 1999, 'New types of antimicrobial compounds produced by Lactobacillus plantarum', Journal of Applied Microbiology, vol. 86, no. 1, pp. 29-35. https://doi.org/10.1046/j.1365-2672.1999.00632.x

New types of antimicrobial compounds produced by Lactobacillus plantarum. / Niku-Paavola, Marja-Leena (Corresponding Author); Laitila, Arja; Mattila-Sandholm, Tiina; Haikara, Auli.

In: Journal of Applied Microbiology, Vol. 86, No. 1, 1999, p. 29-35.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - New types of antimicrobial compounds produced by Lactobacillus plantarum

AU - Niku-Paavola, Marja-Leena

AU - Laitila, Arja

AU - Mattila-Sandholm, Tiina

AU - Haikara, Auli

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N2 - New types of antimicrobial compounds were identified in the culture filtrate of Lactobacillus plantarum VTT E‐78076. Activity was detected in the low molecular mass fraction separated by gel chromatography. This fraction totally inhibited the growth of the Gram‐negative test organism, Pantoea agglomerans (Enterobacter agglomerans) VTT E‐90396. Characteristic compounds from this fraction were identified by GC/MS‐analysis and the identification was confirmed using pure commercial reference compounds in identical chromatographs and in antimicrobial tests. The active fraction included benzoic acid (CAS 65–85–0), 5‐methyl‐2,4‐imidazolidinedione (CAS 616–03–5, methylhydantoin), tetrahydro‐4‐hydroxy‐4‐methyl‐2H‐pyran‐2‐one (CAS 674–26–0, mevalonolactone) and 3‐(2‐methylpropyl)‐2,5‐piperazinedione (CAS 5845–67–0, cyclo(glycyl‐l‐leucyl)). These compounds in concentrations of 10 ppm inhibited growth of the test organism by 10–15% when acting separately, but 100% when all were applied together with 1% lactic acid. The inhibition was 40% by 1% lactic acid alone. The compounds were also active against Fusarium avenaceum (Gibberella avenacea) VTT D‐80147. The inhibition was 10–15% by separate compounds in concentrations of 10 ppm and maximally 20% in combinations. Fungal growth was not inhibited by lactic acid. Inhibition by unfractionated Lact. plantarum culture filtrate was 37% and by the low molecular mass fraction, 27%.

AB - New types of antimicrobial compounds were identified in the culture filtrate of Lactobacillus plantarum VTT E‐78076. Activity was detected in the low molecular mass fraction separated by gel chromatography. This fraction totally inhibited the growth of the Gram‐negative test organism, Pantoea agglomerans (Enterobacter agglomerans) VTT E‐90396. Characteristic compounds from this fraction were identified by GC/MS‐analysis and the identification was confirmed using pure commercial reference compounds in identical chromatographs and in antimicrobial tests. The active fraction included benzoic acid (CAS 65–85–0), 5‐methyl‐2,4‐imidazolidinedione (CAS 616–03–5, methylhydantoin), tetrahydro‐4‐hydroxy‐4‐methyl‐2H‐pyran‐2‐one (CAS 674–26–0, mevalonolactone) and 3‐(2‐methylpropyl)‐2,5‐piperazinedione (CAS 5845–67–0, cyclo(glycyl‐l‐leucyl)). These compounds in concentrations of 10 ppm inhibited growth of the test organism by 10–15% when acting separately, but 100% when all were applied together with 1% lactic acid. The inhibition was 40% by 1% lactic acid alone. The compounds were also active against Fusarium avenaceum (Gibberella avenacea) VTT D‐80147. The inhibition was 10–15% by separate compounds in concentrations of 10 ppm and maximally 20% in combinations. Fungal growth was not inhibited by lactic acid. Inhibition by unfractionated Lact. plantarum culture filtrate was 37% and by the low molecular mass fraction, 27%.

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DO - 10.1046/j.1365-2672.1999.00632.x

M3 - Article

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JO - Journal of Applied Microbiology

JF - Journal of Applied Microbiology

SN - 1364-5072

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