TY - JOUR
T1 - Newly isolated sporopollenin microcages from Cedrus libani and Pinus nigra as carrier for Oxaliplatin; xCELLigence RTCA-based release assay
AU - Mujtaba, Muhammad
AU - Yilmaz, Bahar Akyuz
AU - Cansaran-Duman, Demet
AU - Akyuz, Lalehan
AU - Yangın, Sevcan
AU - Kaya, Murat
AU - Çeter, Talip
AU - Khawar, Khalid Mahmood
N1 - Funding Information:
MM would like to thanks the scientific and technological council of Turkey (TÜBİTAK) for supporting his thesis under program number TÜBİTAK-2215. The authors would like to acknowledge the technical and human support provided by Biotechnology Institute, Ankara University, and Scientific and Technological Application and Research Center, Aksaray University, Turkey.
Funding Information:
MM would like to thanks the scientific and technological council of Turkey (T?B?TAK) for supporting his thesis under program number T?B?TAK-2215. The authors would like to acknowledge the technical and human support provided by Biotechnology Institute, Ankara University, and Scientific and Technological Application and Research Center, Aksaray University, Turkey.
Publisher Copyright:
© 2021, The Author(s), under exclusive licence to Springer-Verlag GmbH, DE part of Springer Nature.
PY - 2022/1
Y1 - 2022/1
N2 - Sporopollenin-mediated control drug delivery has been studied extensively owing to its desirable physicochemical and biological properties. Herein, sporopollenin was successfully extracted from C. libani and P. nigra pollens followed by loading of a commonly known anticancer drug Oxaliplatin. Drug loading and physicochemical features were confirmed by using light microscopy, FT-IR, SEM and TGA. For the first-time, real-time cell analyzer system xCELLigence was employed to record the Oxaliplatin loaded sporopollenin-mediated cell death (CaCo-2 and Vero cells) in real time. Both the release assays confirmed the slow release of oxaliplatin from sporopollenin for around 40–45 h. The expression of MYC and FOXO-3 genes has been significantly increased in CaCo2 cell and decreased non-cancerous Vero cell confirming the fact that sporopollenin-mediated control release of oxaliplatin is promoting apoptosis cell death preventing the spread of negative effects on nearby healthy cells. All the results suggested that C. libani and P. nigra can be suitable candidates for the slow delivery of drugs.
AB - Sporopollenin-mediated control drug delivery has been studied extensively owing to its desirable physicochemical and biological properties. Herein, sporopollenin was successfully extracted from C. libani and P. nigra pollens followed by loading of a commonly known anticancer drug Oxaliplatin. Drug loading and physicochemical features were confirmed by using light microscopy, FT-IR, SEM and TGA. For the first-time, real-time cell analyzer system xCELLigence was employed to record the Oxaliplatin loaded sporopollenin-mediated cell death (CaCo-2 and Vero cells) in real time. Both the release assays confirmed the slow release of oxaliplatin from sporopollenin for around 40–45 h. The expression of MYC and FOXO-3 genes has been significantly increased in CaCo2 cell and decreased non-cancerous Vero cell confirming the fact that sporopollenin-mediated control release of oxaliplatin is promoting apoptosis cell death preventing the spread of negative effects on nearby healthy cells. All the results suggested that C. libani and P. nigra can be suitable candidates for the slow delivery of drugs.
KW - Controlled-drug release
KW - Real-time cell analyzer
KW - Sporopollenin
KW - xCELLigence
UR - http://www.scopus.com/inward/record.url?scp=85098644215&partnerID=8YFLogxK
U2 - 10.1007/s00289-020-03531-7
DO - 10.1007/s00289-020-03531-7
M3 - Article
AN - SCOPUS:85098644215
SN - 0170-0839
VL - 79
SP - 519
EP - 540
JO - Polymer Bulletin
JF - Polymer Bulletin
ER -
