Nile Red staining of phytoplankton neutral lipids: species-specific fluorescence kinetics in various solvents

Katariina Natunen (Corresponding Author), Jukka Seppälä, Dagmar Schwenk, Heiko Rischer, Kristian Spilling, Timo Tamminen

Research output: Contribution to journalArticleScientificpeer-review

10 Citations (Scopus)

Abstract

Nile Red (NR) staining potentially offers a simple method for monitoring lipid accumulation in microalgal cultivation. However, variable staining efficiencies and methods have been reported. The effect of dimethyl sulfoxide (DMSO), ethylene glycol (EG) and glycerol on NR penetration with four different phytoplankton species representing different taxonomical groups was studied. Treatment with the solvents enhanced the NR fluorescence of the diatom Phaeodactylum tricornutum during kinetic fluorescence measurements, but high concentrations of solvents were needed. None of the solvents improved NR staining of the green alga Chlorella pyrenoidosa and Scenedesmus obliquus, which are known to be difficult to stain due to their thick and rigid cell walls. The naked Isochrysis sp. cells stained best without solvents. The results confirm that NR staining protocol needs to be optimized for each species.
Original languageEnglish
Pages (from-to)1161-1168
JournalJournal of Applied Phycology
Volume27
Issue number3
DOIs
Publication statusPublished - 2015
MoE publication typeA1 Journal article-refereed

Fingerprint

fluorescence
lipid
phytoplankton
triacylglycerols
kinetics
Chlorella pyrenoidosa
Isochrysis
Scenedesmus obliquus
Phaeodactylum tricornutum
Bacillariophyceae
ethylene glycol
dimethyl sulfoxide
green alga
ethylene
Chlorophyta
dyes
glycerol
diatom
penetration
cell walls

Keywords

  • fluorescence kinetics
  • neutral lipids
  • phytoplankton
  • solvents

Cite this

Natunen, Katariina ; Seppälä, Jukka ; Schwenk, Dagmar ; Rischer, Heiko ; Spilling, Kristian ; Tamminen, Timo. / Nile Red staining of phytoplankton neutral lipids: species-specific fluorescence kinetics in various solvents. In: Journal of Applied Phycology. 2015 ; Vol. 27, No. 3. pp. 1161-1168.
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abstract = "Nile Red (NR) staining potentially offers a simple method for monitoring lipid accumulation in microalgal cultivation. However, variable staining efficiencies and methods have been reported. The effect of dimethyl sulfoxide (DMSO), ethylene glycol (EG) and glycerol on NR penetration with four different phytoplankton species representing different taxonomical groups was studied. Treatment with the solvents enhanced the NR fluorescence of the diatom Phaeodactylum tricornutum during kinetic fluorescence measurements, but high concentrations of solvents were needed. None of the solvents improved NR staining of the green alga Chlorella pyrenoidosa and Scenedesmus obliquus, which are known to be difficult to stain due to their thick and rigid cell walls. The naked Isochrysis sp. cells stained best without solvents. The results confirm that NR staining protocol needs to be optimized for each species.",
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Nile Red staining of phytoplankton neutral lipids: species-specific fluorescence kinetics in various solvents. / Natunen, Katariina (Corresponding Author); Seppälä, Jukka; Schwenk, Dagmar; Rischer, Heiko; Spilling, Kristian; Tamminen, Timo.

In: Journal of Applied Phycology, Vol. 27, No. 3, 2015, p. 1161-1168.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Nile Red staining of phytoplankton neutral lipids: species-specific fluorescence kinetics in various solvents

AU - Natunen, Katariina

AU - Seppälä, Jukka

AU - Schwenk, Dagmar

AU - Rischer, Heiko

AU - Spilling, Kristian

AU - Tamminen, Timo

PY - 2015

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N2 - Nile Red (NR) staining potentially offers a simple method for monitoring lipid accumulation in microalgal cultivation. However, variable staining efficiencies and methods have been reported. The effect of dimethyl sulfoxide (DMSO), ethylene glycol (EG) and glycerol on NR penetration with four different phytoplankton species representing different taxonomical groups was studied. Treatment with the solvents enhanced the NR fluorescence of the diatom Phaeodactylum tricornutum during kinetic fluorescence measurements, but high concentrations of solvents were needed. None of the solvents improved NR staining of the green alga Chlorella pyrenoidosa and Scenedesmus obliquus, which are known to be difficult to stain due to their thick and rigid cell walls. The naked Isochrysis sp. cells stained best without solvents. The results confirm that NR staining protocol needs to be optimized for each species.

AB - Nile Red (NR) staining potentially offers a simple method for monitoring lipid accumulation in microalgal cultivation. However, variable staining efficiencies and methods have been reported. The effect of dimethyl sulfoxide (DMSO), ethylene glycol (EG) and glycerol on NR penetration with four different phytoplankton species representing different taxonomical groups was studied. Treatment with the solvents enhanced the NR fluorescence of the diatom Phaeodactylum tricornutum during kinetic fluorescence measurements, but high concentrations of solvents were needed. None of the solvents improved NR staining of the green alga Chlorella pyrenoidosa and Scenedesmus obliquus, which are known to be difficult to stain due to their thick and rigid cell walls. The naked Isochrysis sp. cells stained best without solvents. The results confirm that NR staining protocol needs to be optimized for each species.

KW - fluorescence kinetics

KW - neutral lipids

KW - phytoplankton

KW - solvents

U2 - 10.1007/s10811-014-0404-5

DO - 10.1007/s10811-014-0404-5

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VL - 27

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JO - Journal of Applied Phycology

JF - Journal of Applied Phycology

SN - 0921-8971

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ER -