NMR spectroscopic analysis of exopolysaccharides produced by Leuconostoc citreum and Weissella confusa

Ndegwa Henry Maina (Corresponding Author), Maija Tenkanen, Hannu Maaheimo, Riikka Juvonen, Liisa Virkki

Research output: Contribution to journalArticleScientificpeer-review

115 Citations (Scopus)

Abstract

Dextrans are the main exopolysaccharides produced by Leuconostoc species. Other dextran-producing lactic acid bacteria include Streptococci, Lactobacilli, and Weissella species. Commercial production and structural analysis has focused mainly on dextrans from Leuconostoc species, particularly on Leuconostoc mesenteroides strains. In this study, we used NMR spectroscopy techniques to analyze the structures of dextrans produced by Leuconostoc citreum E497 and Weissella confusa E392. The dextrans were compared to that of L. mesenteroides B512F produced under the same conditions. Generally, W. confusa E392 showed better growth and produced more EPS than did L. citreum E497 and L. mesenteroides B512F. Both L. citreum E497 and W. confusa E392 produced a class 1 dextran. Dextran from L. citreum E497 contained about 11% α-(1→2) and about 3.5% α-(1→3)-linked branches whereas dextran from W. confusa E392 was linear with only a few (2.7%) α-(1→3)-linked branches. Dextran from W. confusa E392 was found to be more linear than that of L. mesenteroides B512F, which, according to the present study, contained about 4.1% α-(1→3)-linked branches. Functionality, whether physiological or technological, depends on the structure of the polysaccharide. Dextran from L. citreum E497 may be useful as a source of prebiotic gluco-oligosaccharides with α-(1→2)-linked branches, whereas W. confusa E392 could be a suitable alternative to widely used L. mesenteroides B512F in the production of linear dextran.
Original languageEnglish
Pages (from-to)1446-1455
Number of pages10
JournalCarbohydrate Research
Volume343
Issue number9
DOIs
Publication statusPublished - 2008
MoE publication typeA1 Journal article-refereed

Fingerprint

Weissella
Leuconostoc
Spectroscopic analysis
Dextrans
Nuclear magnetic resonance
Prebiotics
Lactobacillus
Streptococcus
Oligosaccharides
Structural analysis
Nuclear magnetic resonance spectroscopy
Polysaccharides
Lactic Acid
Bacteria

Keywords

  • Exopolysaccharides
  • Dextrans
  • Leuconostoc citreum
  • Leuconostoc mesenteroides
  • Weissella confusa
  • H2BC

Cite this

@article{deafabefb26c42a8a6f3320a7b918f7a,
title = "NMR spectroscopic analysis of exopolysaccharides produced by Leuconostoc citreum and Weissella confusa",
abstract = "Dextrans are the main exopolysaccharides produced by Leuconostoc species. Other dextran-producing lactic acid bacteria include Streptococci, Lactobacilli, and Weissella species. Commercial production and structural analysis has focused mainly on dextrans from Leuconostoc species, particularly on Leuconostoc mesenteroides strains. In this study, we used NMR spectroscopy techniques to analyze the structures of dextrans produced by Leuconostoc citreum E497 and Weissella confusa E392. The dextrans were compared to that of L. mesenteroides B512F produced under the same conditions. Generally, W. confusa E392 showed better growth and produced more EPS than did L. citreum E497 and L. mesenteroides B512F. Both L. citreum E497 and W. confusa E392 produced a class 1 dextran. Dextran from L. citreum E497 contained about 11{\%} α-(1→2) and about 3.5{\%} α-(1→3)-linked branches whereas dextran from W. confusa E392 was linear with only a few (2.7{\%}) α-(1→3)-linked branches. Dextran from W. confusa E392 was found to be more linear than that of L. mesenteroides B512F, which, according to the present study, contained about 4.1{\%} α-(1→3)-linked branches. Functionality, whether physiological or technological, depends on the structure of the polysaccharide. Dextran from L. citreum E497 may be useful as a source of prebiotic gluco-oligosaccharides with α-(1→2)-linked branches, whereas W. confusa E392 could be a suitable alternative to widely used L. mesenteroides B512F in the production of linear dextran.",
keywords = "Exopolysaccharides, Dextrans, Leuconostoc citreum, Leuconostoc mesenteroides, Weissella confusa, H2BC",
author = "Maina, {Ndegwa Henry} and Maija Tenkanen and Hannu Maaheimo and Riikka Juvonen and Liisa Virkki",
year = "2008",
doi = "10.1016/j.carres.2008.04.012",
language = "English",
volume = "343",
pages = "1446--1455",
journal = "Carbohydrate Research",
issn = "0008-6215",
publisher = "Elsevier",
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}

NMR spectroscopic analysis of exopolysaccharides produced by Leuconostoc citreum and Weissella confusa. / Maina, Ndegwa Henry (Corresponding Author); Tenkanen, Maija; Maaheimo, Hannu; Juvonen, Riikka; Virkki, Liisa.

In: Carbohydrate Research, Vol. 343, No. 9, 2008, p. 1446-1455.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - NMR spectroscopic analysis of exopolysaccharides produced by Leuconostoc citreum and Weissella confusa

AU - Maina, Ndegwa Henry

AU - Tenkanen, Maija

AU - Maaheimo, Hannu

AU - Juvonen, Riikka

AU - Virkki, Liisa

PY - 2008

Y1 - 2008

N2 - Dextrans are the main exopolysaccharides produced by Leuconostoc species. Other dextran-producing lactic acid bacteria include Streptococci, Lactobacilli, and Weissella species. Commercial production and structural analysis has focused mainly on dextrans from Leuconostoc species, particularly on Leuconostoc mesenteroides strains. In this study, we used NMR spectroscopy techniques to analyze the structures of dextrans produced by Leuconostoc citreum E497 and Weissella confusa E392. The dextrans were compared to that of L. mesenteroides B512F produced under the same conditions. Generally, W. confusa E392 showed better growth and produced more EPS than did L. citreum E497 and L. mesenteroides B512F. Both L. citreum E497 and W. confusa E392 produced a class 1 dextran. Dextran from L. citreum E497 contained about 11% α-(1→2) and about 3.5% α-(1→3)-linked branches whereas dextran from W. confusa E392 was linear with only a few (2.7%) α-(1→3)-linked branches. Dextran from W. confusa E392 was found to be more linear than that of L. mesenteroides B512F, which, according to the present study, contained about 4.1% α-(1→3)-linked branches. Functionality, whether physiological or technological, depends on the structure of the polysaccharide. Dextran from L. citreum E497 may be useful as a source of prebiotic gluco-oligosaccharides with α-(1→2)-linked branches, whereas W. confusa E392 could be a suitable alternative to widely used L. mesenteroides B512F in the production of linear dextran.

AB - Dextrans are the main exopolysaccharides produced by Leuconostoc species. Other dextran-producing lactic acid bacteria include Streptococci, Lactobacilli, and Weissella species. Commercial production and structural analysis has focused mainly on dextrans from Leuconostoc species, particularly on Leuconostoc mesenteroides strains. In this study, we used NMR spectroscopy techniques to analyze the structures of dextrans produced by Leuconostoc citreum E497 and Weissella confusa E392. The dextrans were compared to that of L. mesenteroides B512F produced under the same conditions. Generally, W. confusa E392 showed better growth and produced more EPS than did L. citreum E497 and L. mesenteroides B512F. Both L. citreum E497 and W. confusa E392 produced a class 1 dextran. Dextran from L. citreum E497 contained about 11% α-(1→2) and about 3.5% α-(1→3)-linked branches whereas dextran from W. confusa E392 was linear with only a few (2.7%) α-(1→3)-linked branches. Dextran from W. confusa E392 was found to be more linear than that of L. mesenteroides B512F, which, according to the present study, contained about 4.1% α-(1→3)-linked branches. Functionality, whether physiological or technological, depends on the structure of the polysaccharide. Dextran from L. citreum E497 may be useful as a source of prebiotic gluco-oligosaccharides with α-(1→2)-linked branches, whereas W. confusa E392 could be a suitable alternative to widely used L. mesenteroides B512F in the production of linear dextran.

KW - Exopolysaccharides

KW - Dextrans

KW - Leuconostoc citreum

KW - Leuconostoc mesenteroides

KW - Weissella confusa

KW - H2BC

U2 - 10.1016/j.carres.2008.04.012

DO - 10.1016/j.carres.2008.04.012

M3 - Article

VL - 343

SP - 1446

EP - 1455

JO - Carbohydrate Research

JF - Carbohydrate Research

SN - 0008-6215

IS - 9

ER -