Non-oxidative antibacterial activity of bovine neutrophil granule proteins towards mastitis pathogens

Paul Grieve, Tiina Mattila

Research output: Contribution to journalArticleScientificpeer-review

3 Citations (Scopus)

Abstract

Acid extracts of bovine neutrophil granules displayed potent antibacterial activity towards a number of mastitis pathogens in vitro. Killing of pathogens by acid extractable granule protein was dependent on incubation time, protein concentration, bacterial cell load, pH and ionic strength. Gram‐negative and Gram‐positive organisms showed variable sensitivity to granule extract. Strains of Staphylococcus aureus were the most resistant of tested organisms to granule extract. Gram‐negative organisms were neither consistently more nor less sensitive than Gram‐positive organisms. Maximal killing of Gram‐positive pathogens, after 30 minutes exposure to granule extract at 37 °C, occurred between pH 7.0 and 8.0. The Gram‐negative organism Escherichia coli B117 was more sensitive to neutrophil granule extract at pH 5.0.
Original languageEnglish
Pages (from-to)500-508
JournalZentralblatt für Veterinärmedizin, Reihe B: Infektions- und Invasionskrankheiten, Bakteriologie, Virologie, Parasitologie, Hygiene, Lebensmittelhygiene, Pathologie
Volume36
Issue number1-10
DOIs
Publication statusPublished - 1989
MoE publication typeA1 Journal article-refereed

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Mastitis
mastitis
neutrophils
granules
Neutrophils
pathogens
cattle
organisms
extracts
Proteins
Acids
proteins
Bacterial Load
Osmolar Concentration
Staphylococcus aureus
Escherichia coli
acids
ionic strength
cells

Cite this

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title = "Non-oxidative antibacterial activity of bovine neutrophil granule proteins towards mastitis pathogens",
abstract = "Acid extracts of bovine neutrophil granules displayed potent antibacterial activity towards a number of mastitis pathogens in vitro. Killing of pathogens by acid extractable granule protein was dependent on incubation time, protein concentration, bacterial cell load, pH and ionic strength. Gram‐negative and Gram‐positive organisms showed variable sensitivity to granule extract. Strains of Staphylococcus aureus were the most resistant of tested organisms to granule extract. Gram‐negative organisms were neither consistently more nor less sensitive than Gram‐positive organisms. Maximal killing of Gram‐positive pathogens, after 30 minutes exposure to granule extract at 37 °C, occurred between pH 7.0 and 8.0. The Gram‐negative organism Escherichia coli B117 was more sensitive to neutrophil granule extract at pH 5.0.",
author = "Paul Grieve and Tiina Mattila",
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T1 - Non-oxidative antibacterial activity of bovine neutrophil granule proteins towards mastitis pathogens

AU - Grieve, Paul

AU - Mattila, Tiina

PY - 1989

Y1 - 1989

N2 - Acid extracts of bovine neutrophil granules displayed potent antibacterial activity towards a number of mastitis pathogens in vitro. Killing of pathogens by acid extractable granule protein was dependent on incubation time, protein concentration, bacterial cell load, pH and ionic strength. Gram‐negative and Gram‐positive organisms showed variable sensitivity to granule extract. Strains of Staphylococcus aureus were the most resistant of tested organisms to granule extract. Gram‐negative organisms were neither consistently more nor less sensitive than Gram‐positive organisms. Maximal killing of Gram‐positive pathogens, after 30 minutes exposure to granule extract at 37 °C, occurred between pH 7.0 and 8.0. The Gram‐negative organism Escherichia coli B117 was more sensitive to neutrophil granule extract at pH 5.0.

AB - Acid extracts of bovine neutrophil granules displayed potent antibacterial activity towards a number of mastitis pathogens in vitro. Killing of pathogens by acid extractable granule protein was dependent on incubation time, protein concentration, bacterial cell load, pH and ionic strength. Gram‐negative and Gram‐positive organisms showed variable sensitivity to granule extract. Strains of Staphylococcus aureus were the most resistant of tested organisms to granule extract. Gram‐negative organisms were neither consistently more nor less sensitive than Gram‐positive organisms. Maximal killing of Gram‐positive pathogens, after 30 minutes exposure to granule extract at 37 °C, occurred between pH 7.0 and 8.0. The Gram‐negative organism Escherichia coli B117 was more sensitive to neutrophil granule extract at pH 5.0.

U2 - 10.1111/j.1439-0450.1989.tb00636.x

DO - 10.1111/j.1439-0450.1989.tb00636.x

M3 - Article

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SP - 500

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JO - Zoonoses and Public Health

JF - Zoonoses and Public Health

SN - 1863-1959

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ER -