Non-oxidative antibacterial activity of bovine neutrophil granule proteins towards mastitis pathogens

Paul Grieve, Tiina Mattila

Research output: Contribution to journalArticleScientificpeer-review

3 Citations (Scopus)


Acid extracts of bovine neutrophil granules displayed potent antibacterial activity towards a number of mastitis pathogens in vitro. Killing of pathogens by acid extractable granule protein was dependent on incubation time, protein concentration, bacterial cell load, pH and ionic strength. Gram‐negative and Gram‐positive organisms showed variable sensitivity to granule extract. Strains of Staphylococcus aureus were the most resistant of tested organisms to granule extract. Gram‐negative organisms were neither consistently more nor less sensitive than Gram‐positive organisms. Maximal killing of Gram‐positive pathogens, after 30 minutes exposure to granule extract at 37 °C, occurred between pH 7.0 and 8.0. The Gram‐negative organism Escherichia coli B117 was more sensitive to neutrophil granule extract at pH 5.0.
Original languageEnglish
Pages (from-to)500-508
JournalZentralblatt für Veterinärmedizin, Reihe B: Infektions- und Invasionskrankheiten, Bakteriologie, Virologie, Parasitologie, Hygiene, Lebensmittelhygiene, Pathologie
Issue number1-10
Publication statusPublished - 1989
MoE publication typeA1 Journal article-refereed


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