Nontargeted metabolite profiling discriminates diet-specific biomarkers for consumption of whole grains, fatty fish, and bilberries in a randomized controlled trial

Kati Hanhineva (Corresponding Author), Maria A. Lankinen, Anna Pedret, Ursula Schwab, Marjukka Kolehmainen, Jussi Paananen, Vanessa de Mello, Rosa Sola, Marko Lehtonen, Kaisa Poutanen, Matti Uusitupa, Hannu Mykkänen

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Abstract

Background: Nontargeted metabolite profiling allows for concomitant examination of a wide range of metabolite species, elucidating the metabolic alterations caused by dietary interventions. Objective: The aim of the current study was to investigate the effects of dietary modifications on the basis of increasing consumption of whole grains, fatty fish, and bilberries on plasma metabolite profiles to identify applicable biomarkers for dietary intake and endogenous metabolism. Methods: Metabolite profiling analysis was performed on fasting plasma samples collected in a 12-wk parallel-group intervention with 106 participants with features of metabolic syndrome who were randomly assigned to 3 dietary interventions: 1) whole-grain products, fatty fish, and bilberries [healthy diet (HD)]; 2) a whole-grain–enriched diet with the same grain products as in the HD intervention but with no change in fish or berry consumption; and 3) refined-wheat breads and restrictions on fish and berries (control diet). In addition, correlation analyses were conducted with the food intake data to define the food items correlating with the biomarker candidates. Results: Nontargeted metabolite profiling showed marked differences in fasting plasma after the intervention diets compared with the control diet. In both intervention groups, a significant increase was observed in 2 signals identified as glucuronidated alk(en)-ylresorcinols [corrected P value (Pcorr) <0.05], which correlated strongly with the intake of whole-grain products (r = 0.63, P <0.001). In addition, the HD intervention increased the signals for furan fatty acids [3-carboxy-4-methyl-5-propyl-2-furanpropionic acid (CMPF)], hippuric acid, and various lipid species incorporating polyunsaturated fatty acids (Pcorr <0.05). In particular, plasma CMPF correlated strongly with the intake of fish (r = 0.47, P <0.001) but not with intakes of any other foods. Conclusions: Novel biomarkers of the intake of health-beneficial food items included in the Nordic diet were identified by the metabolite profiling of fasting plasma and confirmed by the correlation analyses with dietary records. The one with the most potential was CMPF, which was shown to be a highly specific biomarker for fatty fish intake. This trial was registered at clinicaltrials.gov as NCT00573781.
Original languageEnglish
Pages (from-to)7 - 17
JournalJournal of Nutrition
Volume145
Issue number1
DOIs
Publication statusPublished - 2015
MoE publication typeA1 Journal article-refereed

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Vaccinium myrtillus
Fishes
Randomized Controlled Trials
Biomarkers
Diet
Fasting
Food
Fruit
Fish Products
Diet Therapy
Diet Records
Bread
Unsaturated Fatty Acids
Triticum
Fatty Acids
Eating
Whole Grains
Lipids
Health
Healthy Diet

Keywords

  • diet
  • nutrition
  • metabolomics
  • biomarkers
  • alkylresorcinol

Cite this

Hanhineva, Kati ; Lankinen, Maria A. ; Pedret, Anna ; Schwab, Ursula ; Kolehmainen, Marjukka ; Paananen, Jussi ; Mello, Vanessa de ; Sola, Rosa ; Lehtonen, Marko ; Poutanen, Kaisa ; Uusitupa, Matti ; Mykkänen, Hannu. / Nontargeted metabolite profiling discriminates diet-specific biomarkers for consumption of whole grains, fatty fish, and bilberries in a randomized controlled trial. In: Journal of Nutrition. 2015 ; Vol. 145, No. 1. pp. 7 - 17.
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title = "Nontargeted metabolite profiling discriminates diet-specific biomarkers for consumption of whole grains, fatty fish, and bilberries in a randomized controlled trial",
abstract = "Background: Nontargeted metabolite profiling allows for concomitant examination of a wide range of metabolite species, elucidating the metabolic alterations caused by dietary interventions. Objective: The aim of the current study was to investigate the effects of dietary modifications on the basis of increasing consumption of whole grains, fatty fish, and bilberries on plasma metabolite profiles to identify applicable biomarkers for dietary intake and endogenous metabolism. Methods: Metabolite profiling analysis was performed on fasting plasma samples collected in a 12-wk parallel-group intervention with 106 participants with features of metabolic syndrome who were randomly assigned to 3 dietary interventions: 1) whole-grain products, fatty fish, and bilberries [healthy diet (HD)]; 2) a whole-grain–enriched diet with the same grain products as in the HD intervention but with no change in fish or berry consumption; and 3) refined-wheat breads and restrictions on fish and berries (control diet). In addition, correlation analyses were conducted with the food intake data to define the food items correlating with the biomarker candidates. Results: Nontargeted metabolite profiling showed marked differences in fasting plasma after the intervention diets compared with the control diet. In both intervention groups, a significant increase was observed in 2 signals identified as glucuronidated alk(en)-ylresorcinols [corrected P value (Pcorr) <0.05], which correlated strongly with the intake of whole-grain products (r = 0.63, P <0.001). In addition, the HD intervention increased the signals for furan fatty acids [3-carboxy-4-methyl-5-propyl-2-furanpropionic acid (CMPF)], hippuric acid, and various lipid species incorporating polyunsaturated fatty acids (Pcorr <0.05). In particular, plasma CMPF correlated strongly with the intake of fish (r = 0.47, P <0.001) but not with intakes of any other foods. Conclusions: Novel biomarkers of the intake of health-beneficial food items included in the Nordic diet were identified by the metabolite profiling of fasting plasma and confirmed by the correlation analyses with dietary records. The one with the most potential was CMPF, which was shown to be a highly specific biomarker for fatty fish intake. This trial was registered at clinicaltrials.gov as NCT00573781.",
keywords = "diet, nutrition, metabolomics, biomarkers, alkylresorcinol",
author = "Kati Hanhineva and Lankinen, {Maria A.} and Anna Pedret and Ursula Schwab and Marjukka Kolehmainen and Jussi Paananen and Mello, {Vanessa de} and Rosa Sola and Marko Lehtonen and Kaisa Poutanen and Matti Uusitupa and Hannu Mykk{\"a}nen",
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pages = "7 -- 17",
journal = "Journal of Nutrition",
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Hanhineva, K, Lankinen, MA, Pedret, A, Schwab, U, Kolehmainen, M, Paananen, J, Mello, VD, Sola, R, Lehtonen, M, Poutanen, K, Uusitupa, M & Mykkänen, H 2015, 'Nontargeted metabolite profiling discriminates diet-specific biomarkers for consumption of whole grains, fatty fish, and bilberries in a randomized controlled trial', Journal of Nutrition, vol. 145, no. 1, pp. 7 - 17. https://doi.org/10.3945/jn.114.196840

Nontargeted metabolite profiling discriminates diet-specific biomarkers for consumption of whole grains, fatty fish, and bilberries in a randomized controlled trial. / Hanhineva, Kati (Corresponding Author); Lankinen, Maria A.; Pedret, Anna; Schwab, Ursula; Kolehmainen, Marjukka; Paananen, Jussi; Mello, Vanessa de; Sola, Rosa; Lehtonen, Marko; Poutanen, Kaisa; Uusitupa, Matti; Mykkänen, Hannu.

In: Journal of Nutrition, Vol. 145, No. 1, 2015, p. 7 - 17.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Nontargeted metabolite profiling discriminates diet-specific biomarkers for consumption of whole grains, fatty fish, and bilberries in a randomized controlled trial

AU - Hanhineva, Kati

AU - Lankinen, Maria A.

AU - Pedret, Anna

AU - Schwab, Ursula

AU - Kolehmainen, Marjukka

AU - Paananen, Jussi

AU - Mello, Vanessa de

AU - Sola, Rosa

AU - Lehtonen, Marko

AU - Poutanen, Kaisa

AU - Uusitupa, Matti

AU - Mykkänen, Hannu

PY - 2015

Y1 - 2015

N2 - Background: Nontargeted metabolite profiling allows for concomitant examination of a wide range of metabolite species, elucidating the metabolic alterations caused by dietary interventions. Objective: The aim of the current study was to investigate the effects of dietary modifications on the basis of increasing consumption of whole grains, fatty fish, and bilberries on plasma metabolite profiles to identify applicable biomarkers for dietary intake and endogenous metabolism. Methods: Metabolite profiling analysis was performed on fasting plasma samples collected in a 12-wk parallel-group intervention with 106 participants with features of metabolic syndrome who were randomly assigned to 3 dietary interventions: 1) whole-grain products, fatty fish, and bilberries [healthy diet (HD)]; 2) a whole-grain–enriched diet with the same grain products as in the HD intervention but with no change in fish or berry consumption; and 3) refined-wheat breads and restrictions on fish and berries (control diet). In addition, correlation analyses were conducted with the food intake data to define the food items correlating with the biomarker candidates. Results: Nontargeted metabolite profiling showed marked differences in fasting plasma after the intervention diets compared with the control diet. In both intervention groups, a significant increase was observed in 2 signals identified as glucuronidated alk(en)-ylresorcinols [corrected P value (Pcorr) <0.05], which correlated strongly with the intake of whole-grain products (r = 0.63, P <0.001). In addition, the HD intervention increased the signals for furan fatty acids [3-carboxy-4-methyl-5-propyl-2-furanpropionic acid (CMPF)], hippuric acid, and various lipid species incorporating polyunsaturated fatty acids (Pcorr <0.05). In particular, plasma CMPF correlated strongly with the intake of fish (r = 0.47, P <0.001) but not with intakes of any other foods. Conclusions: Novel biomarkers of the intake of health-beneficial food items included in the Nordic diet were identified by the metabolite profiling of fasting plasma and confirmed by the correlation analyses with dietary records. The one with the most potential was CMPF, which was shown to be a highly specific biomarker for fatty fish intake. This trial was registered at clinicaltrials.gov as NCT00573781.

AB - Background: Nontargeted metabolite profiling allows for concomitant examination of a wide range of metabolite species, elucidating the metabolic alterations caused by dietary interventions. Objective: The aim of the current study was to investigate the effects of dietary modifications on the basis of increasing consumption of whole grains, fatty fish, and bilberries on plasma metabolite profiles to identify applicable biomarkers for dietary intake and endogenous metabolism. Methods: Metabolite profiling analysis was performed on fasting plasma samples collected in a 12-wk parallel-group intervention with 106 participants with features of metabolic syndrome who were randomly assigned to 3 dietary interventions: 1) whole-grain products, fatty fish, and bilberries [healthy diet (HD)]; 2) a whole-grain–enriched diet with the same grain products as in the HD intervention but with no change in fish or berry consumption; and 3) refined-wheat breads and restrictions on fish and berries (control diet). In addition, correlation analyses were conducted with the food intake data to define the food items correlating with the biomarker candidates. Results: Nontargeted metabolite profiling showed marked differences in fasting plasma after the intervention diets compared with the control diet. In both intervention groups, a significant increase was observed in 2 signals identified as glucuronidated alk(en)-ylresorcinols [corrected P value (Pcorr) <0.05], which correlated strongly with the intake of whole-grain products (r = 0.63, P <0.001). In addition, the HD intervention increased the signals for furan fatty acids [3-carboxy-4-methyl-5-propyl-2-furanpropionic acid (CMPF)], hippuric acid, and various lipid species incorporating polyunsaturated fatty acids (Pcorr <0.05). In particular, plasma CMPF correlated strongly with the intake of fish (r = 0.47, P <0.001) but not with intakes of any other foods. Conclusions: Novel biomarkers of the intake of health-beneficial food items included in the Nordic diet were identified by the metabolite profiling of fasting plasma and confirmed by the correlation analyses with dietary records. The one with the most potential was CMPF, which was shown to be a highly specific biomarker for fatty fish intake. This trial was registered at clinicaltrials.gov as NCT00573781.

KW - diet

KW - nutrition

KW - metabolomics

KW - biomarkers

KW - alkylresorcinol

U2 - 10.3945/jn.114.196840

DO - 10.3945/jn.114.196840

M3 - Article

VL - 145

SP - 7

EP - 17

JO - Journal of Nutrition

JF - Journal of Nutrition

SN - 0022-3166

IS - 1

ER -