Novel, dynamic on-line analytical separation system for dissolution of drugs from poly(lactic acid) nanoparticles

Anne Helle, Samuli Hirsjärvi, Leena Peltonen, Jouni Hirvonen, Susanne K. Wiedmer (Corresponding Author), Tuulia Hyötyläinen (Corresponding Author)

Research output: Contribution to journalArticleScientificpeer-review

11 Citations (Scopus)

Abstract

A novel method for investigating drug release in a dynamic manner from nanoparticles including, but not limited to, biodegradable poly(lactic acid) (PLA) is reported. The PLA nanoparticles were prepared by the nanoprecipitation method. Two poorly soluble drugs, beclomethasone dipropionate (BDP) and indomethacin, were encapsulated into PLA nanoparticles, and their dissolution from the nanoparticles were followed in a dynamic way. The on-line method comprised a short column (vessel) packed with the PLA nanoparticles, on-line connected to an analytical liquid chromatographic column via a multiport switching valve equipped with two loops. The system allowed monitoring of the drug release profiles in real time, and the conditions for the drug release could be precisely controlled and easily changed. The effects of solvent composition and temperature on the rate of dissolution of the drugs from the PLA nanoparticles were investigated. The system proved to be linear for the drugs tested over the concentration range 10–3000 ng (n = 6, R2 = 0.999 and 0.997 for indomethacin and beclomethasone, respectively) and repeatable (RSD of peak areas <0.5%). The recoveries of the dissolution study were quantitative (120 and 103% for indomethacin and beclomethasone, respectively).
Original languageEnglish
Pages (from-to)125-130
JournalJournal of Pharmaceutical and Biomedical Analysis
Volume51
Issue number1
DOIs
Publication statusPublished - 2010
MoE publication typeA1 Journal article-refereed

Fingerprint

Nanoparticles
Dissolution
Beclomethasone
Indomethacin
Pharmaceutical Preparations
poly(lactic acid)
Drug Liberation
Recovery
Temperature
Monitoring
Liquids
Chemical analysis

Keywords

  • poly(lactic acid)
  • beclomethasone dipropionate
  • indomethacin
  • liquid chromatography
  • dissolution
  • drug release

Cite this

Helle, Anne ; Hirsjärvi, Samuli ; Peltonen, Leena ; Hirvonen, Jouni ; Wiedmer, Susanne K. ; Hyötyläinen, Tuulia. / Novel, dynamic on-line analytical separation system for dissolution of drugs from poly(lactic acid) nanoparticles. In: Journal of Pharmaceutical and Biomedical Analysis. 2010 ; Vol. 51, No. 1. pp. 125-130.
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Novel, dynamic on-line analytical separation system for dissolution of drugs from poly(lactic acid) nanoparticles. / Helle, Anne; Hirsjärvi, Samuli; Peltonen, Leena; Hirvonen, Jouni; Wiedmer, Susanne K. (Corresponding Author); Hyötyläinen, Tuulia (Corresponding Author).

In: Journal of Pharmaceutical and Biomedical Analysis, Vol. 51, No. 1, 2010, p. 125-130.

Research output: Contribution to journalArticleScientificpeer-review

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AU - Helle, Anne

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AU - Wiedmer, Susanne K.

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