Novel polypyrrole-coated polylactide scaffolds enhance adipose stem cell proliferation and early osteogenic differentiation

Jani Pelto, M. Björninen, A. Pälli, E. Talvitie, J. Hyttinen, B. Mannerström, R. Suuronen Seppänen, M. Kellomäki, S. Miettinen, S. Haimi

Research output: Contribution to journalArticleScientificpeer-review

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Abstract

An electrically conductive polypyrrole (PPy) doped with a bioactive agent is an emerging functional biomaterial for tissue engineering. We therefore used chondroitin sulfate (CS)-doped PPy coating to modify initially electrically insulating polylactide resulting in novel osteogenic scaffolds. In situ chemical oxidative polymerization was used to obtain electrically conductive PPy coating on poly-96L/4D-lactide (PLA) nonwoven scaffolds. The coated scaffolds were characterized and their electrical conductivity was evaluated in hydrolysis. The ability of the coated and conductive scaffolds to enhance proliferation and osteogenic differentiation of human adipose stem cells (hASCs) under electrical stimulation (ES) in three-dimensional (3D) geometry was compared to the noncoated PLA scaffolds. Electrical conductivity of PPy-coated PLA scaffolds (PLA-PPy) was evident at the beginning of hydrolysis, but decreased during the first week of incubation due to de-doping. PLA-PPy scaffolds enhanced hASC proliferation significantly compared to the plain PLA scaffolds at 7 and 14 days. Furthermore, the alkaline phosphatase (ALP) activity of the hASCs was generally higher in PLA-PPy seeded scaffolds, but due to patient variation, no statistical significance could be determined. ES did not have a significant effect on hASCs. This study highlights the potential of novel PPy-coated PLA scaffolds in bone tissue engineering.
Original languageEnglish
Pages (from-to)882-892
Number of pages11
JournalTissue Engineering: Part A
Volume19
Issue number7-8
DOIs
Publication statusPublished - 2013
MoE publication typeA1 Journal article-refereed

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Polypyrroles
Cell proliferation
Scaffolds (biology)
Stem cells
Scaffolds
Stem Cells
Cell Proliferation
Electric Conductivity
Tissue engineering
Tissue Engineering
Hydrolysis
Electric Stimulation
Coatings
polypyrrole
poly(lactide)
Phosphatases
Chondroitin Sulfates
Biomaterials
Biocompatible Materials
Human Activities

Cite this

Pelto, Jani ; Björninen, M. ; Pälli, A. ; Talvitie, E. ; Hyttinen, J. ; Mannerström, B. ; Suuronen Seppänen, R. ; Kellomäki, M. ; Miettinen, S. ; Haimi, S. / Novel polypyrrole-coated polylactide scaffolds enhance adipose stem cell proliferation and early osteogenic differentiation. In: Tissue Engineering: Part A. 2013 ; Vol. 19, No. 7-8. pp. 882-892.
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title = "Novel polypyrrole-coated polylactide scaffolds enhance adipose stem cell proliferation and early osteogenic differentiation",
abstract = "An electrically conductive polypyrrole (PPy) doped with a bioactive agent is an emerging functional biomaterial for tissue engineering. We therefore used chondroitin sulfate (CS)-doped PPy coating to modify initially electrically insulating polylactide resulting in novel osteogenic scaffolds. In situ chemical oxidative polymerization was used to obtain electrically conductive PPy coating on poly-96L/4D-lactide (PLA) nonwoven scaffolds. The coated scaffolds were characterized and their electrical conductivity was evaluated in hydrolysis. The ability of the coated and conductive scaffolds to enhance proliferation and osteogenic differentiation of human adipose stem cells (hASCs) under electrical stimulation (ES) in three-dimensional (3D) geometry was compared to the noncoated PLA scaffolds. Electrical conductivity of PPy-coated PLA scaffolds (PLA-PPy) was evident at the beginning of hydrolysis, but decreased during the first week of incubation due to de-doping. PLA-PPy scaffolds enhanced hASC proliferation significantly compared to the plain PLA scaffolds at 7 and 14 days. Furthermore, the alkaline phosphatase (ALP) activity of the hASCs was generally higher in PLA-PPy seeded scaffolds, but due to patient variation, no statistical significance could be determined. ES did not have a significant effect on hASCs. This study highlights the potential of novel PPy-coated PLA scaffolds in bone tissue engineering.",
author = "Jani Pelto and M. Bj{\"o}rninen and A. P{\"a}lli and E. Talvitie and J. Hyttinen and B. Mannerstr{\"o}m and {Suuronen Sepp{\"a}nen}, R. and M. Kellom{\"a}ki and S. Miettinen and S. Haimi",
year = "2013",
doi = "10.1089/ten.tea.2012.0111",
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Pelto, J, Björninen, M, Pälli, A, Talvitie, E, Hyttinen, J, Mannerström, B, Suuronen Seppänen, R, Kellomäki, M, Miettinen, S & Haimi, S 2013, 'Novel polypyrrole-coated polylactide scaffolds enhance adipose stem cell proliferation and early osteogenic differentiation', Tissue Engineering: Part A, vol. 19, no. 7-8, pp. 882-892. https://doi.org/10.1089/ten.tea.2012.0111

Novel polypyrrole-coated polylactide scaffolds enhance adipose stem cell proliferation and early osteogenic differentiation. / Pelto, Jani; Björninen, M.; Pälli, A.; Talvitie, E.; Hyttinen, J.; Mannerström, B.; Suuronen Seppänen, R.; Kellomäki, M.; Miettinen, S.; Haimi, S.

In: Tissue Engineering: Part A, Vol. 19, No. 7-8, 2013, p. 882-892.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Novel polypyrrole-coated polylactide scaffolds enhance adipose stem cell proliferation and early osteogenic differentiation

AU - Pelto, Jani

AU - Björninen, M.

AU - Pälli, A.

AU - Talvitie, E.

AU - Hyttinen, J.

AU - Mannerström, B.

AU - Suuronen Seppänen, R.

AU - Kellomäki, M.

AU - Miettinen, S.

AU - Haimi, S.

PY - 2013

Y1 - 2013

N2 - An electrically conductive polypyrrole (PPy) doped with a bioactive agent is an emerging functional biomaterial for tissue engineering. We therefore used chondroitin sulfate (CS)-doped PPy coating to modify initially electrically insulating polylactide resulting in novel osteogenic scaffolds. In situ chemical oxidative polymerization was used to obtain electrically conductive PPy coating on poly-96L/4D-lactide (PLA) nonwoven scaffolds. The coated scaffolds were characterized and their electrical conductivity was evaluated in hydrolysis. The ability of the coated and conductive scaffolds to enhance proliferation and osteogenic differentiation of human adipose stem cells (hASCs) under electrical stimulation (ES) in three-dimensional (3D) geometry was compared to the noncoated PLA scaffolds. Electrical conductivity of PPy-coated PLA scaffolds (PLA-PPy) was evident at the beginning of hydrolysis, but decreased during the first week of incubation due to de-doping. PLA-PPy scaffolds enhanced hASC proliferation significantly compared to the plain PLA scaffolds at 7 and 14 days. Furthermore, the alkaline phosphatase (ALP) activity of the hASCs was generally higher in PLA-PPy seeded scaffolds, but due to patient variation, no statistical significance could be determined. ES did not have a significant effect on hASCs. This study highlights the potential of novel PPy-coated PLA scaffolds in bone tissue engineering.

AB - An electrically conductive polypyrrole (PPy) doped with a bioactive agent is an emerging functional biomaterial for tissue engineering. We therefore used chondroitin sulfate (CS)-doped PPy coating to modify initially electrically insulating polylactide resulting in novel osteogenic scaffolds. In situ chemical oxidative polymerization was used to obtain electrically conductive PPy coating on poly-96L/4D-lactide (PLA) nonwoven scaffolds. The coated scaffolds were characterized and their electrical conductivity was evaluated in hydrolysis. The ability of the coated and conductive scaffolds to enhance proliferation and osteogenic differentiation of human adipose stem cells (hASCs) under electrical stimulation (ES) in three-dimensional (3D) geometry was compared to the noncoated PLA scaffolds. Electrical conductivity of PPy-coated PLA scaffolds (PLA-PPy) was evident at the beginning of hydrolysis, but decreased during the first week of incubation due to de-doping. PLA-PPy scaffolds enhanced hASC proliferation significantly compared to the plain PLA scaffolds at 7 and 14 days. Furthermore, the alkaline phosphatase (ALP) activity of the hASCs was generally higher in PLA-PPy seeded scaffolds, but due to patient variation, no statistical significance could be determined. ES did not have a significant effect on hASCs. This study highlights the potential of novel PPy-coated PLA scaffolds in bone tissue engineering.

U2 - 10.1089/ten.tea.2012.0111

DO - 10.1089/ten.tea.2012.0111

M3 - Article

VL - 19

SP - 882

EP - 892

JO - Tissue Engineering: Part A

JF - Tissue Engineering: Part A

SN - 1937-3341

IS - 7-8

ER -