Novel tools for the engineering of secondary metabolite pathways in plants

Research output: Contribution to conferenceConference articleScientific

Abstract

We developed a novel technology platform that allows the high throughput selecting and testing of genes involved in the production of plant secondary metabolites. It can be applied (i) to enhance the production of marketed high-value pharmaceuticals in plant cell cultures (ii) to develop reliable and reproducible sources of plant-derived molecules with potential pharmaceutical value, and (iii) to increase the chemical diversity of plant based molecules through Combinatorial Biochemistry. We designed this novel approach using tobacco BY-2 cell culture as a model system, in which a cDNA-AFLP based transcript profiling technique is linked with targeted metabolite profiling of these cells to simultaneously identify genes involved in nicotine alkaloid metabolism on a genome-wide scale. A few examples will be given of how overexpressing some novel genes can be used either to tailor cell cultures to enhance the production of nicotine alkaloids or to direct biosynthetic pathways in related plant species. Furthermore, these novel genes may play an important role in secondary metabolism as master regulators in general. The great advantage of our technology is its universal application to any plant or cell culture of interest (e.g. rare medicinal plants) without pre-existing gene sequence databases.
Original languageEnglish
Number of pages1
Publication statusPublished - 2004
MoE publication typeNot Eligible
EventKazusa DNA Research Institute Workshop Tomato: A New Model Plant in the Genomics Era - Chiba, Japan
Duration: 22 Mar 200423 Mar 2004

Workshop

WorkshopKazusa DNA Research Institute Workshop Tomato
CountryJapan
CityChiba
Period22/03/0423/03/04

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secondary metabolites
engineering
cell culture
nicotine
alkaloids
genes
drugs
metabolism
plant cultural practices
biochemistry
amplified fragment length polymorphism
medicinal plants
biochemical pathways
tobacco
metabolites
nucleotide sequences
genome
testing
cells
methodology

Cite this

Oksman-Caldentey, K-M., & Inze, D. (2004). Novel tools for the engineering of secondary metabolite pathways in plants. Paper presented at Kazusa DNA Research Institute Workshop Tomato, Chiba, Japan.
Oksman-Caldentey, Kirsi-Marja ; Inze, Dirk. / Novel tools for the engineering of secondary metabolite pathways in plants. Paper presented at Kazusa DNA Research Institute Workshop Tomato, Chiba, Japan.1 p.
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title = "Novel tools for the engineering of secondary metabolite pathways in plants",
abstract = "We developed a novel technology platform that allows the high throughput selecting and testing of genes involved in the production of plant secondary metabolites. It can be applied (i) to enhance the production of marketed high-value pharmaceuticals in plant cell cultures (ii) to develop reliable and reproducible sources of plant-derived molecules with potential pharmaceutical value, and (iii) to increase the chemical diversity of plant based molecules through Combinatorial Biochemistry. We designed this novel approach using tobacco BY-2 cell culture as a model system, in which a cDNA-AFLP based transcript profiling technique is linked with targeted metabolite profiling of these cells to simultaneously identify genes involved in nicotine alkaloid metabolism on a genome-wide scale. A few examples will be given of how overexpressing some novel genes can be used either to tailor cell cultures to enhance the production of nicotine alkaloids or to direct biosynthetic pathways in related plant species. Furthermore, these novel genes may play an important role in secondary metabolism as master regulators in general. The great advantage of our technology is its universal application to any plant or cell culture of interest (e.g. rare medicinal plants) without pre-existing gene sequence databases.",
author = "Kirsi-Marja Oksman-Caldentey and Dirk Inze",
year = "2004",
language = "English",
note = "Kazusa DNA Research Institute Workshop Tomato : A New Model Plant in the Genomics Era ; Conference date: 22-03-2004 Through 23-03-2004",

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Oksman-Caldentey, K-M & Inze, D 2004, 'Novel tools for the engineering of secondary metabolite pathways in plants' Paper presented at Kazusa DNA Research Institute Workshop Tomato, Chiba, Japan, 22/03/04 - 23/03/04, .

Novel tools for the engineering of secondary metabolite pathways in plants. / Oksman-Caldentey, Kirsi-Marja; Inze, Dirk.

2004. Paper presented at Kazusa DNA Research Institute Workshop Tomato, Chiba, Japan.

Research output: Contribution to conferenceConference articleScientific

TY - CONF

T1 - Novel tools for the engineering of secondary metabolite pathways in plants

AU - Oksman-Caldentey, Kirsi-Marja

AU - Inze, Dirk

PY - 2004

Y1 - 2004

N2 - We developed a novel technology platform that allows the high throughput selecting and testing of genes involved in the production of plant secondary metabolites. It can be applied (i) to enhance the production of marketed high-value pharmaceuticals in plant cell cultures (ii) to develop reliable and reproducible sources of plant-derived molecules with potential pharmaceutical value, and (iii) to increase the chemical diversity of plant based molecules through Combinatorial Biochemistry. We designed this novel approach using tobacco BY-2 cell culture as a model system, in which a cDNA-AFLP based transcript profiling technique is linked with targeted metabolite profiling of these cells to simultaneously identify genes involved in nicotine alkaloid metabolism on a genome-wide scale. A few examples will be given of how overexpressing some novel genes can be used either to tailor cell cultures to enhance the production of nicotine alkaloids or to direct biosynthetic pathways in related plant species. Furthermore, these novel genes may play an important role in secondary metabolism as master regulators in general. The great advantage of our technology is its universal application to any plant or cell culture of interest (e.g. rare medicinal plants) without pre-existing gene sequence databases.

AB - We developed a novel technology platform that allows the high throughput selecting and testing of genes involved in the production of plant secondary metabolites. It can be applied (i) to enhance the production of marketed high-value pharmaceuticals in plant cell cultures (ii) to develop reliable and reproducible sources of plant-derived molecules with potential pharmaceutical value, and (iii) to increase the chemical diversity of plant based molecules through Combinatorial Biochemistry. We designed this novel approach using tobacco BY-2 cell culture as a model system, in which a cDNA-AFLP based transcript profiling technique is linked with targeted metabolite profiling of these cells to simultaneously identify genes involved in nicotine alkaloid metabolism on a genome-wide scale. A few examples will be given of how overexpressing some novel genes can be used either to tailor cell cultures to enhance the production of nicotine alkaloids or to direct biosynthetic pathways in related plant species. Furthermore, these novel genes may play an important role in secondary metabolism as master regulators in general. The great advantage of our technology is its universal application to any plant or cell culture of interest (e.g. rare medicinal plants) without pre-existing gene sequence databases.

M3 - Conference article

ER -

Oksman-Caldentey K-M, Inze D. Novel tools for the engineering of secondary metabolite pathways in plants. 2004. Paper presented at Kazusa DNA Research Institute Workshop Tomato, Chiba, Japan.