Nuclear DNA content of Pinus sylvestris (L.) as determined by laser flow cytometry

Jari Valkonen, Markku Nygren, Anne Ylönen, Leena Mannonen

Research output: Contribution to journalArticleScientificpeer-review

16 Citations (Scopus)

Abstract

Nuclear DNA content was determined in nuclei isolated from needles, stems and roots of in vitro grown seedlings and from megagametophytes and embryo of mature seeds in three accessions of Pinus sylvestris L. One accession was from Inari, northern Finland at timber line, and two accessions were from the Alpine region in Italy. Nuclei were mechanically isolated by a chopping method, stained with propidium iodide, and DNA content was determined using an EPICS PROFILE laser flow cytometer. Nuclei isolated from leaves of barley (Hordeum vulgare L. cv. Sultan; 2C=11.12 pg) were used as an internal standard for measurement of pine nuclei. Mean 1C nuclear DNA content of P. sylvestris was 27.88 pg as determined from megagametophyte tissue. Mean 2C value was 52.25 pg as determined from stem and root tissue, and 55.58 pg as determined from embryo tissue. The ratio of 2C to 1C value was 1.87 and 1.99, respectively. Extracts of nuclei from needles contained propidium iodide-absorbing debris which may have interfered with measurements and resulted in lower 2C values than those obtained from stem and root.
Original languageEnglish
Pages (from-to)203-207
Number of pages5
JournalGenetica
Volume92
Issue number3
DOIs
Publication statusPublished - 1994
MoE publication typeA1 Journal article-refereed

Fingerprint

Pinus sylvestris
nuclear genome
Ovule
lasers
flow cytometry
Flow Cytometry
Lasers
Propidium
Hordeum
embryo (plant)
stems
Needles
DNA
Embryonic Structures
chopping
Finland
Seedlings
Hordeum vulgare
Italy
Seeds

Cite this

Valkonen, Jari ; Nygren, Markku ; Ylönen, Anne ; Mannonen, Leena. / Nuclear DNA content of Pinus sylvestris (L.) as determined by laser flow cytometry. In: Genetica. 1994 ; Vol. 92, No. 3. pp. 203-207.
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title = "Nuclear DNA content of Pinus sylvestris (L.) as determined by laser flow cytometry",
abstract = "Nuclear DNA content was determined in nuclei isolated from needles, stems and roots of in vitro grown seedlings and from megagametophytes and embryo of mature seeds in three accessions of Pinus sylvestris L. One accession was from Inari, northern Finland at timber line, and two accessions were from the Alpine region in Italy. Nuclei were mechanically isolated by a chopping method, stained with propidium iodide, and DNA content was determined using an EPICS PROFILE laser flow cytometer. Nuclei isolated from leaves of barley (Hordeum vulgare L. cv. Sultan; 2C=11.12 pg) were used as an internal standard for measurement of pine nuclei. Mean 1C nuclear DNA content of P. sylvestris was 27.88 pg as determined from megagametophyte tissue. Mean 2C value was 52.25 pg as determined from stem and root tissue, and 55.58 pg as determined from embryo tissue. The ratio of 2C to 1C value was 1.87 and 1.99, respectively. Extracts of nuclei from needles contained propidium iodide-absorbing debris which may have interfered with measurements and resulted in lower 2C values than those obtained from stem and root.",
author = "Jari Valkonen and Markku Nygren and Anne Yl{\"o}nen and Leena Mannonen",
year = "1994",
doi = "10.1007/BF00132539",
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Valkonen, J, Nygren, M, Ylönen, A & Mannonen, L 1994, 'Nuclear DNA content of Pinus sylvestris (L.) as determined by laser flow cytometry', Genetica, vol. 92, no. 3, pp. 203-207. https://doi.org/10.1007/BF00132539

Nuclear DNA content of Pinus sylvestris (L.) as determined by laser flow cytometry. / Valkonen, Jari; Nygren, Markku; Ylönen, Anne; Mannonen, Leena.

In: Genetica, Vol. 92, No. 3, 1994, p. 203-207.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Nuclear DNA content of Pinus sylvestris (L.) as determined by laser flow cytometry

AU - Valkonen, Jari

AU - Nygren, Markku

AU - Ylönen, Anne

AU - Mannonen, Leena

PY - 1994

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AB - Nuclear DNA content was determined in nuclei isolated from needles, stems and roots of in vitro grown seedlings and from megagametophytes and embryo of mature seeds in three accessions of Pinus sylvestris L. One accession was from Inari, northern Finland at timber line, and two accessions were from the Alpine region in Italy. Nuclei were mechanically isolated by a chopping method, stained with propidium iodide, and DNA content was determined using an EPICS PROFILE laser flow cytometer. Nuclei isolated from leaves of barley (Hordeum vulgare L. cv. Sultan; 2C=11.12 pg) were used as an internal standard for measurement of pine nuclei. Mean 1C nuclear DNA content of P. sylvestris was 27.88 pg as determined from megagametophyte tissue. Mean 2C value was 52.25 pg as determined from stem and root tissue, and 55.58 pg as determined from embryo tissue. The ratio of 2C to 1C value was 1.87 and 1.99, respectively. Extracts of nuclei from needles contained propidium iodide-absorbing debris which may have interfered with measurements and resulted in lower 2C values than those obtained from stem and root.

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