Nutrient uptake and growth of an embryogenic and a non-embryogenic cell line of birch (Betula pendula Roth.)

Anna-Maria Nuutila, Veli Kauppinen

    Research output: Contribution to journalArticleScientificpeer-review

    8 Citations (Scopus)

    Abstract

    The nutrient uptake of an embryogenic and of a non-embryogenic cell line of birch (Betula pendula Roth.) during cell growth and embryo production was studied in suspension culture. The embryogenic and non-embryogenic cell suspensions grew differently in the same medium. The non-embryogenic cell line started to grow without any lag period after the inoculation. It rapidly hydrolyzed sucrose in the medium to glucose and fructose and consumed the glucose as carbon source. The concentration of fructose in the medium decreased only after the depletion of glucose. The embryogenic cell line also rapidly hydrolyzed the sucrose to glucose and fructose, but the monosaccharides were consumed only after the embryos started to germinate after three weeks of culture. Both monosaccharides were then taken up at the same rate.
    Original languageEnglish
    Pages (from-to)7-13
    Number of pages7
    JournalPlant Cell, Tissue and Organ Culture
    Volume30
    Issue number1
    DOIs
    Publication statusPublished - 1992
    MoE publication typeA1 Journal article-refereed

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    Betula pendula
    Betula
    nutrient uptake
    cell lines
    fructose
    glucose
    monosaccharides
    sucrose
    cell suspension culture
    cell growth
    carbon

    Cite this

    @article{5b93a1cef0b84ce9aec310637f8aed95,
    title = "Nutrient uptake and growth of an embryogenic and a non-embryogenic cell line of birch (Betula pendula Roth.)",
    abstract = "The nutrient uptake of an embryogenic and of a non-embryogenic cell line of birch (Betula pendula Roth.) during cell growth and embryo production was studied in suspension culture. The embryogenic and non-embryogenic cell suspensions grew differently in the same medium. The non-embryogenic cell line started to grow without any lag period after the inoculation. It rapidly hydrolyzed sucrose in the medium to glucose and fructose and consumed the glucose as carbon source. The concentration of fructose in the medium decreased only after the depletion of glucose. The embryogenic cell line also rapidly hydrolyzed the sucrose to glucose and fructose, but the monosaccharides were consumed only after the embryos started to germinate after three weeks of culture. Both monosaccharides were then taken up at the same rate.",
    author = "Anna-Maria Nuutila and Veli Kauppinen",
    note = "Project code: BIO9001",
    year = "1992",
    doi = "10.1007/BF00039996",
    language = "English",
    volume = "30",
    pages = "7--13",
    journal = "Plant Cell, Tissue and Organ Culture",
    issn = "0167-6857",
    publisher = "Springer",
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    }

    Nutrient uptake and growth of an embryogenic and a non-embryogenic cell line of birch (Betula pendula Roth.). / Nuutila, Anna-Maria; Kauppinen, Veli.

    In: Plant Cell, Tissue and Organ Culture, Vol. 30, No. 1, 1992, p. 7-13.

    Research output: Contribution to journalArticleScientificpeer-review

    TY - JOUR

    T1 - Nutrient uptake and growth of an embryogenic and a non-embryogenic cell line of birch (Betula pendula Roth.)

    AU - Nuutila, Anna-Maria

    AU - Kauppinen, Veli

    N1 - Project code: BIO9001

    PY - 1992

    Y1 - 1992

    N2 - The nutrient uptake of an embryogenic and of a non-embryogenic cell line of birch (Betula pendula Roth.) during cell growth and embryo production was studied in suspension culture. The embryogenic and non-embryogenic cell suspensions grew differently in the same medium. The non-embryogenic cell line started to grow without any lag period after the inoculation. It rapidly hydrolyzed sucrose in the medium to glucose and fructose and consumed the glucose as carbon source. The concentration of fructose in the medium decreased only after the depletion of glucose. The embryogenic cell line also rapidly hydrolyzed the sucrose to glucose and fructose, but the monosaccharides were consumed only after the embryos started to germinate after three weeks of culture. Both monosaccharides were then taken up at the same rate.

    AB - The nutrient uptake of an embryogenic and of a non-embryogenic cell line of birch (Betula pendula Roth.) during cell growth and embryo production was studied in suspension culture. The embryogenic and non-embryogenic cell suspensions grew differently in the same medium. The non-embryogenic cell line started to grow without any lag period after the inoculation. It rapidly hydrolyzed sucrose in the medium to glucose and fructose and consumed the glucose as carbon source. The concentration of fructose in the medium decreased only after the depletion of glucose. The embryogenic cell line also rapidly hydrolyzed the sucrose to glucose and fructose, but the monosaccharides were consumed only after the embryos started to germinate after three weeks of culture. Both monosaccharides were then taken up at the same rate.

    U2 - 10.1007/BF00039996

    DO - 10.1007/BF00039996

    M3 - Article

    VL - 30

    SP - 7

    EP - 13

    JO - Plant Cell, Tissue and Organ Culture

    JF - Plant Cell, Tissue and Organ Culture

    SN - 0167-6857

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    ER -