Abstract
Colony radial growth rates and specific growth rates of three related Ashbya gossypii strains ATCC10895, IMI31268, MUCL29450 and an unrelated strain, CBS109.26, were measured on various carbon and nitrogen sources at pH 4.5 and pH 6.5 to elucidate physiological growth requirements and strain differences. All strains grew on yeast extract or ammonium as nitrogen sources, but not on nitrate. Substantial growth at pH 4.5 was observed only on complex medium. D-Glucose, glycerol and starch were utilised as carbon sources. Ethanol was produced during growth on glycerol. Conversion of xylose into xylitol demonstrates that the xylose reductase is active. Phenotypic differences between related strains were greater than expected. We demonstrate that A. gossypii utilizes ammonium as sole nitrogen source at pH 6.5, facilitating further physiological studies using chemically defined media in the future.
| Original language | English |
|---|---|
| Pages (from-to) | 582-589 |
| Journal | Journal of Basic Microbiology |
| Volume | 52 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - 1 Oct 2012 |
| MoE publication type | A1 Journal article-refereed |
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Keywords
- Ashbya gossypii
- Carbon utilization
- Colony radial growth rate
- Ethanol
- Nitrogen utilization
- PH
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Nutritional requirements and strain heterogeneity in Ashbya gossypii. / Ribeiro, Orquídea; Domingues, Lucília; Penttilä, Merja; Wiebe, Marilyn G. (Corresponding Author).
In: Journal of Basic Microbiology, Vol. 52, No. 5, 01.10.2012, p. 582-589.Research output: Contribution to journal › Article › Scientific › peer-review
TY - JOUR
T1 - Nutritional requirements and strain heterogeneity in Ashbya gossypii
AU - Ribeiro, Orquídea
AU - Domingues, Lucília
AU - Penttilä, Merja
AU - Wiebe, Marilyn G.
N1 - CA2: TK402 TK400 SDA: BIC ISI: MICROBIOLOGY
PY - 2012/10/1
Y1 - 2012/10/1
N2 - Colony radial growth rates and specific growth rates of three related Ashbya gossypii strains ATCC10895, IMI31268, MUCL29450 and an unrelated strain, CBS109.26, were measured on various carbon and nitrogen sources at pH 4.5 and pH 6.5 to elucidate physiological growth requirements and strain differences. All strains grew on yeast extract or ammonium as nitrogen sources, but not on nitrate. Substantial growth at pH 4.5 was observed only on complex medium. D-Glucose, glycerol and starch were utilised as carbon sources. Ethanol was produced during growth on glycerol. Conversion of xylose into xylitol demonstrates that the xylose reductase is active. Phenotypic differences between related strains were greater than expected. We demonstrate that A. gossypii utilizes ammonium as sole nitrogen source at pH 6.5, facilitating further physiological studies using chemically defined media in the future.
AB - Colony radial growth rates and specific growth rates of three related Ashbya gossypii strains ATCC10895, IMI31268, MUCL29450 and an unrelated strain, CBS109.26, were measured on various carbon and nitrogen sources at pH 4.5 and pH 6.5 to elucidate physiological growth requirements and strain differences. All strains grew on yeast extract or ammonium as nitrogen sources, but not on nitrate. Substantial growth at pH 4.5 was observed only on complex medium. D-Glucose, glycerol and starch were utilised as carbon sources. Ethanol was produced during growth on glycerol. Conversion of xylose into xylitol demonstrates that the xylose reductase is active. Phenotypic differences between related strains were greater than expected. We demonstrate that A. gossypii utilizes ammonium as sole nitrogen source at pH 6.5, facilitating further physiological studies using chemically defined media in the future.
KW - Ashbya gossypii
KW - Carbon utilization
KW - Colony radial growth rate
KW - Ethanol
KW - Nitrogen utilization
KW - PH
UR - http://www.scopus.com/inward/record.url?scp=84867377195&partnerID=8YFLogxK
U2 - 10.1002/jobm.201100383
DO - 10.1002/jobm.201100383
M3 - Article
C2 - 22143993
AN - SCOPUS:84867377195
VL - 52
SP - 582
EP - 589
JO - Journal of Basic Microbiology
JF - Journal of Basic Microbiology
SN - 0233-111X
IS - 5
ER -