Abstract
| Original language | English |
|---|---|
| Pages (from-to) | 494-506 |
| Number of pages | 12 |
| Journal | Glycobiology |
| Volume | 24 |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - 2014 |
| MoE publication type | A1 Journal article-refereed |
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Keywords
- Arabidopsis
- GlucUronic acid substitution of xylan (GUX)
- irregular xylem
- secondary wall
- xylan acetylation
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O-Acetylation of glucuronoxylan in Arabidopsis thaliana wild type and its change in xylan biosynthesis mutants. / Chong, S L (Corresponding Author); Virkki, L; Maaheimo, Hannu; Juvonen, M; Derba-Maceluch, M; Koutaniemi, S; Roach, M; Sundberg, B; Tuomainen, P; Mellerowicz, E J; Tenkanen, M.
In: Glycobiology, Vol. 24, No. 6, 2014, p. 494-506.Research output: Contribution to journal › Article › Scientific › peer-review
TY - JOUR
T1 - O-Acetylation of glucuronoxylan in Arabidopsis thaliana wild type and its change in xylan biosynthesis mutants
AU - Chong, S L
AU - Virkki, L
AU - Maaheimo, Hannu
AU - Juvonen, M
AU - Derba-Maceluch, M
AU - Koutaniemi, S
AU - Roach, M
AU - Sundberg, B
AU - Tuomainen, P
AU - Mellerowicz, E J
AU - Tenkanen, M
PY - 2014
Y1 - 2014
N2 - O-Acetylglucuronoxylans (AcGX) in Arabidopsis thaliana carry acetyl residues on the 2-O and/or 3-O positions of the xylopyranosyl (Xylp) units, but the distribution of different O-acetylated Xylp units is partly unclear. We studied a possible correlation of xylan acetylation and the activities of different glycosyltransferases involved in xylan biosynthesis by analyzing the distribution of O-acetyl substituents on AcGX from Arabidopsis wild-type and mutants irx7, irx9-1, irx10, irx14 and gux1gux2. The relative contents of the Xylp structural units were determined with quantitative two-dimensional heteronuclear single quantum coherence nuclear magnetic resonance spectroscopy. In the wild type, the degree of acetylation (DA) was 60%. Mono-and diacetylated Xylp units constituted 44 and 6% of the AcGX backbone, respectively; while (4-O-methyl)-glucopyranosyluronic acid (1?2)-linked Xylp units, most of which also carry 3-Oacetylation, represented 13%. The DA was decreased in irx7, irx9-1 and irx14 due to the decrease in monoacetylation (2-O and 3-O), indicating a relationship between acetylation and other AcGX biosynthetic processes. The possible interactions that could lead to such changes have been discussed. No change in DAwas observed in irx10 and gux1gux2, but monoacetylation was nonetheless elevated in gux1gux2. This indicates that acetylation occurs after addition of GlcpA to the xylan backbone. Mass fragmentation analysis suggests that the prevalent acetylation pattern is the acetyl group added on every other Xylp unit
AB - O-Acetylglucuronoxylans (AcGX) in Arabidopsis thaliana carry acetyl residues on the 2-O and/or 3-O positions of the xylopyranosyl (Xylp) units, but the distribution of different O-acetylated Xylp units is partly unclear. We studied a possible correlation of xylan acetylation and the activities of different glycosyltransferases involved in xylan biosynthesis by analyzing the distribution of O-acetyl substituents on AcGX from Arabidopsis wild-type and mutants irx7, irx9-1, irx10, irx14 and gux1gux2. The relative contents of the Xylp structural units were determined with quantitative two-dimensional heteronuclear single quantum coherence nuclear magnetic resonance spectroscopy. In the wild type, the degree of acetylation (DA) was 60%. Mono-and diacetylated Xylp units constituted 44 and 6% of the AcGX backbone, respectively; while (4-O-methyl)-glucopyranosyluronic acid (1?2)-linked Xylp units, most of which also carry 3-Oacetylation, represented 13%. The DA was decreased in irx7, irx9-1 and irx14 due to the decrease in monoacetylation (2-O and 3-O), indicating a relationship between acetylation and other AcGX biosynthetic processes. The possible interactions that could lead to such changes have been discussed. No change in DAwas observed in irx10 and gux1gux2, but monoacetylation was nonetheless elevated in gux1gux2. This indicates that acetylation occurs after addition of GlcpA to the xylan backbone. Mass fragmentation analysis suggests that the prevalent acetylation pattern is the acetyl group added on every other Xylp unit
KW - Arabidopsis
KW - GlucUronic acid substitution of xylan (GUX)
KW - irregular xylem
KW - secondary wall
KW - xylan acetylation
U2 - 10.1093/glycob/cwu017
DO - 10.1093/glycob/cwu017
M3 - Article
VL - 24
SP - 494
EP - 506
JO - Glycobiology
JF - Glycobiology
SN - 0959-6658
IS - 6
ER -