O-Acetylation of glucuronoxylan in Arabidopsis thaliana wild type and its change in xylan biosynthesis mutants

S L Chong (Corresponding Author), L Virkki, Hannu Maaheimo, M Juvonen, M Derba-Maceluch, S Koutaniemi, M Roach, B Sundberg, P Tuomainen, E J Mellerowicz, M Tenkanen

Research output: Contribution to journalArticleScientificpeer-review

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Abstract

O-Acetylglucuronoxylans (AcGX) in Arabidopsis thaliana carry acetyl residues on the 2-O and/or 3-O positions of the xylopyranosyl (Xylp) units, but the distribution of different O-acetylated Xylp units is partly unclear. We studied a possible correlation of xylan acetylation and the activities of different glycosyltransferases involved in xylan biosynthesis by analyzing the distribution of O-acetyl substituents on AcGX from Arabidopsis wild-type and mutants irx7, irx9-1, irx10, irx14 and gux1gux2. The relative contents of the Xylp structural units were determined with quantitative two-dimensional heteronuclear single quantum coherence nuclear magnetic resonance spectroscopy. In the wild type, the degree of acetylation (DA) was 60%. Mono-and diacetylated Xylp units constituted 44 and 6% of the AcGX backbone, respectively; while (4-O-methyl)-glucopyranosyluronic acid (1?2)-linked Xylp units, most of which also carry 3-Oacetylation, represented 13%. The DA was decreased in irx7, irx9-1 and irx14 due to the decrease in monoacetylation (2-O and 3-O), indicating a relationship between acetylation and other AcGX biosynthetic processes. The possible interactions that could lead to such changes have been discussed. No change in DAwas observed in irx10 and gux1gux2, but monoacetylation was nonetheless elevated in gux1gux2. This indicates that acetylation occurs after addition of GlcpA to the xylan backbone. Mass fragmentation analysis suggests that the prevalent acetylation pattern is the acetyl group added on every other Xylp unit
Original languageEnglish
Pages (from-to)494-506
Number of pages12
JournalGlycobiology
Volume24
Issue number6
DOIs
Publication statusPublished - 2014
MoE publication typeA1 Journal article-refereed

Fingerprint

Xylans
Acetylation
Biosynthesis
Arabidopsis
Glycosyltransferases
Nuclear magnetic resonance spectroscopy
glucuronoxylan
Magnetic Resonance Spectroscopy
Acids

Keywords

  • Arabidopsis
  • GlucUronic acid substitution of xylan (GUX)
  • irregular xylem
  • secondary wall
  • xylan acetylation

Cite this

Chong, S. L., Virkki, L., Maaheimo, H., Juvonen, M., Derba-Maceluch, M., Koutaniemi, S., ... Tenkanen, M. (2014). O-Acetylation of glucuronoxylan in Arabidopsis thaliana wild type and its change in xylan biosynthesis mutants. Glycobiology, 24(6), 494-506. https://doi.org/10.1093/glycob/cwu017
Chong, S L ; Virkki, L ; Maaheimo, Hannu ; Juvonen, M ; Derba-Maceluch, M ; Koutaniemi, S ; Roach, M ; Sundberg, B ; Tuomainen, P ; Mellerowicz, E J ; Tenkanen, M. / O-Acetylation of glucuronoxylan in Arabidopsis thaliana wild type and its change in xylan biosynthesis mutants. In: Glycobiology. 2014 ; Vol. 24, No. 6. pp. 494-506.
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abstract = "O-Acetylglucuronoxylans (AcGX) in Arabidopsis thaliana carry acetyl residues on the 2-O and/or 3-O positions of the xylopyranosyl (Xylp) units, but the distribution of different O-acetylated Xylp units is partly unclear. We studied a possible correlation of xylan acetylation and the activities of different glycosyltransferases involved in xylan biosynthesis by analyzing the distribution of O-acetyl substituents on AcGX from Arabidopsis wild-type and mutants irx7, irx9-1, irx10, irx14 and gux1gux2. The relative contents of the Xylp structural units were determined with quantitative two-dimensional heteronuclear single quantum coherence nuclear magnetic resonance spectroscopy. In the wild type, the degree of acetylation (DA) was 60{\%}. Mono-and diacetylated Xylp units constituted 44 and 6{\%} of the AcGX backbone, respectively; while (4-O-methyl)-glucopyranosyluronic acid (1?2)-linked Xylp units, most of which also carry 3-Oacetylation, represented 13{\%}. The DA was decreased in irx7, irx9-1 and irx14 due to the decrease in monoacetylation (2-O and 3-O), indicating a relationship between acetylation and other AcGX biosynthetic processes. The possible interactions that could lead to such changes have been discussed. No change in DAwas observed in irx10 and gux1gux2, but monoacetylation was nonetheless elevated in gux1gux2. This indicates that acetylation occurs after addition of GlcpA to the xylan backbone. Mass fragmentation analysis suggests that the prevalent acetylation pattern is the acetyl group added on every other Xylp unit",
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Chong, SL, Virkki, L, Maaheimo, H, Juvonen, M, Derba-Maceluch, M, Koutaniemi, S, Roach, M, Sundberg, B, Tuomainen, P, Mellerowicz, EJ & Tenkanen, M 2014, 'O-Acetylation of glucuronoxylan in Arabidopsis thaliana wild type and its change in xylan biosynthesis mutants', Glycobiology, vol. 24, no. 6, pp. 494-506. https://doi.org/10.1093/glycob/cwu017

O-Acetylation of glucuronoxylan in Arabidopsis thaliana wild type and its change in xylan biosynthesis mutants. / Chong, S L (Corresponding Author); Virkki, L; Maaheimo, Hannu; Juvonen, M; Derba-Maceluch, M; Koutaniemi, S; Roach, M; Sundberg, B; Tuomainen, P; Mellerowicz, E J; Tenkanen, M.

In: Glycobiology, Vol. 24, No. 6, 2014, p. 494-506.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - O-Acetylation of glucuronoxylan in Arabidopsis thaliana wild type and its change in xylan biosynthesis mutants

AU - Chong, S L

AU - Virkki, L

AU - Maaheimo, Hannu

AU - Juvonen, M

AU - Derba-Maceluch, M

AU - Koutaniemi, S

AU - Roach, M

AU - Sundberg, B

AU - Tuomainen, P

AU - Mellerowicz, E J

AU - Tenkanen, M

PY - 2014

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N2 - O-Acetylglucuronoxylans (AcGX) in Arabidopsis thaliana carry acetyl residues on the 2-O and/or 3-O positions of the xylopyranosyl (Xylp) units, but the distribution of different O-acetylated Xylp units is partly unclear. We studied a possible correlation of xylan acetylation and the activities of different glycosyltransferases involved in xylan biosynthesis by analyzing the distribution of O-acetyl substituents on AcGX from Arabidopsis wild-type and mutants irx7, irx9-1, irx10, irx14 and gux1gux2. The relative contents of the Xylp structural units were determined with quantitative two-dimensional heteronuclear single quantum coherence nuclear magnetic resonance spectroscopy. In the wild type, the degree of acetylation (DA) was 60%. Mono-and diacetylated Xylp units constituted 44 and 6% of the AcGX backbone, respectively; while (4-O-methyl)-glucopyranosyluronic acid (1?2)-linked Xylp units, most of which also carry 3-Oacetylation, represented 13%. The DA was decreased in irx7, irx9-1 and irx14 due to the decrease in monoacetylation (2-O and 3-O), indicating a relationship between acetylation and other AcGX biosynthetic processes. The possible interactions that could lead to such changes have been discussed. No change in DAwas observed in irx10 and gux1gux2, but monoacetylation was nonetheless elevated in gux1gux2. This indicates that acetylation occurs after addition of GlcpA to the xylan backbone. Mass fragmentation analysis suggests that the prevalent acetylation pattern is the acetyl group added on every other Xylp unit

AB - O-Acetylglucuronoxylans (AcGX) in Arabidopsis thaliana carry acetyl residues on the 2-O and/or 3-O positions of the xylopyranosyl (Xylp) units, but the distribution of different O-acetylated Xylp units is partly unclear. We studied a possible correlation of xylan acetylation and the activities of different glycosyltransferases involved in xylan biosynthesis by analyzing the distribution of O-acetyl substituents on AcGX from Arabidopsis wild-type and mutants irx7, irx9-1, irx10, irx14 and gux1gux2. The relative contents of the Xylp structural units were determined with quantitative two-dimensional heteronuclear single quantum coherence nuclear magnetic resonance spectroscopy. In the wild type, the degree of acetylation (DA) was 60%. Mono-and diacetylated Xylp units constituted 44 and 6% of the AcGX backbone, respectively; while (4-O-methyl)-glucopyranosyluronic acid (1?2)-linked Xylp units, most of which also carry 3-Oacetylation, represented 13%. The DA was decreased in irx7, irx9-1 and irx14 due to the decrease in monoacetylation (2-O and 3-O), indicating a relationship between acetylation and other AcGX biosynthetic processes. The possible interactions that could lead to such changes have been discussed. No change in DAwas observed in irx10 and gux1gux2, but monoacetylation was nonetheless elevated in gux1gux2. This indicates that acetylation occurs after addition of GlcpA to the xylan backbone. Mass fragmentation analysis suggests that the prevalent acetylation pattern is the acetyl group added on every other Xylp unit

KW - Arabidopsis

KW - GlucUronic acid substitution of xylan (GUX)

KW - irregular xylem

KW - secondary wall

KW - xylan acetylation

U2 - 10.1093/glycob/cwu017

DO - 10.1093/glycob/cwu017

M3 - Article

VL - 24

SP - 494

EP - 506

JO - Glycobiology

JF - Glycobiology

SN - 0959-6658

IS - 6

ER -