Oat beta-glucan and xylan hydrolysates as selective substrates for Bifidobacterium and Lactobacillus strains

Johanna Jaskari; Pia Kontula; A. Siitonen; Hannele Jousimies-Somer; Tiina Mattila-Sandholm; Kaisa Poutanen

doi:10.1007/s002530051155

Oat beta-glucan and xylan hydrolysates as selective substrates for Bifidobacterium and Lactobacillus strains

Johanna Jaskari, Pia Kontula, A. Siitonen, Hannele Jousimies-Somer, Tiina Mattila-Sandholm, Kaisa Poutanen (Corresponding Author)

VTT Technical Research Centre of Finland

National Public Health Institute of Finland

Research output: Contribution to journal › Article › Scientific › peer-review

257 Citations (Scopus)

Abstract

Novel oligomers that resist digestion in the upper gut were prepared from oat mixed-linked β-glucan and xylan by enzymatic hydrolysis with lichenase of Bacillus subtilis and xylanase of Trichoderma reesei respectively.
The low-molecular-mass hydrolysis products of β-glucan and xylan were compared with fructooligomers and raffinose in their ability to provide growth substrates for probiotic (Lactobacillus and Bifidobacterium) and intestinal (Bacteroides, Clostridium and Escherichia coli) strains in vitro.
A degradation profile of each carbohydrate and total sugar consumption were analysed with HPLC, and bacterial growth rate with an automatic turbidometer, the Bioscreen C system. β-Glucooligomers and xylooligomers both enhanced the growth of health-promoting probiotic strains as compared with intestinal bacterial growth, but not to a significant level.
Raffinose stimulated the probiotic strains significantly, whereas fructooligomers induced high average growth for intestinal bacteria also.

Original language	English
Pages (from-to)	175-181
Journal	Applied Microbiology and Biotechnology
Volume	49
Issue number	2
DOIs	https://doi.org/10.1007/s002530051155
Publication status	Published - 1998
MoE publication type	A1 Journal article-refereed

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title = "Oat beta-glucan and xylan hydrolysates as selective substrates for Bifidobacterium and Lactobacillus strains",

abstract = "Novel oligomers that resist digestion in the upper gut were prepared from oat mixed-linked β-glucan and xylan by enzymatic hydrolysis with lichenase of Bacillus subtilis and xylanase of Trichoderma reesei respectively. The low-molecular-mass hydrolysis products of β-glucan and xylan were compared with fructooligomers and raffinose in their ability to provide growth substrates for probiotic (Lactobacillus and Bifidobacterium) and intestinal (Bacteroides, Clostridium and Escherichia coli) strains in vitro. A degradation profile of each carbohydrate and total sugar consumption were analysed with HPLC, and bacterial growth rate with an automatic turbidometer, the Bioscreen C system. β-Glucooligomers and xylooligomers both enhanced the growth of health-promoting probiotic strains as compared with intestinal bacterial growth, but not to a significant level. Raffinose stimulated the probiotic strains significantly, whereas fructooligomers induced high average growth for intestinal bacteria also.",

author = "Johanna Jaskari and Pia Kontula and A. Siitonen and Hannele Jousimies-Somer and Tiina Mattila-Sandholm and Kaisa Poutanen",

year = "1998",

doi = "10.1007/s002530051155",

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volume = "49",

pages = "175--181",

journal = "Applied Microbiology and Biotechnology",

issn = "0175-7598",

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T1 - Oat beta-glucan and xylan hydrolysates as selective substrates for Bifidobacterium and Lactobacillus strains

AU - Jaskari, Johanna

AU - Kontula, Pia

AU - Siitonen, A.

AU - Jousimies-Somer, Hannele

AU - Mattila-Sandholm, Tiina

AU - Poutanen, Kaisa

PY - 1998

Y1 - 1998

N2 - Novel oligomers that resist digestion in the upper gut were prepared from oat mixed-linked β-glucan and xylan by enzymatic hydrolysis with lichenase of Bacillus subtilis and xylanase of Trichoderma reesei respectively. The low-molecular-mass hydrolysis products of β-glucan and xylan were compared with fructooligomers and raffinose in their ability to provide growth substrates for probiotic (Lactobacillus and Bifidobacterium) and intestinal (Bacteroides, Clostridium and Escherichia coli) strains in vitro. A degradation profile of each carbohydrate and total sugar consumption were analysed with HPLC, and bacterial growth rate with an automatic turbidometer, the Bioscreen C system. β-Glucooligomers and xylooligomers both enhanced the growth of health-promoting probiotic strains as compared with intestinal bacterial growth, but not to a significant level. Raffinose stimulated the probiotic strains significantly, whereas fructooligomers induced high average growth for intestinal bacteria also.

AB - Novel oligomers that resist digestion in the upper gut were prepared from oat mixed-linked β-glucan and xylan by enzymatic hydrolysis with lichenase of Bacillus subtilis and xylanase of Trichoderma reesei respectively. The low-molecular-mass hydrolysis products of β-glucan and xylan were compared with fructooligomers and raffinose in their ability to provide growth substrates for probiotic (Lactobacillus and Bifidobacterium) and intestinal (Bacteroides, Clostridium and Escherichia coli) strains in vitro. A degradation profile of each carbohydrate and total sugar consumption were analysed with HPLC, and bacterial growth rate with an automatic turbidometer, the Bioscreen C system. β-Glucooligomers and xylooligomers both enhanced the growth of health-promoting probiotic strains as compared with intestinal bacterial growth, but not to a significant level. Raffinose stimulated the probiotic strains significantly, whereas fructooligomers induced high average growth for intestinal bacteria also.

U2 - 10.1007/s002530051155

DO - 10.1007/s002530051155

M3 - Article

VL - 49

SP - 175

EP - 181

JO - Applied Microbiology and Biotechnology

JF - Applied Microbiology and Biotechnology

SN - 0175-7598

IS - 2

ER -

Oat beta-glucan and xylan hydrolysates as selective substrates for Bifidobacterium and Lactobacillus strains

Abstract

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