Oat beta-glucan and xylan hydrolysates as selective substrates for Bifidobacterium and Lactobacillus strains

Johanna Jaskari, Pia Kontula, A. Siitonen, Hannele Jousimies-Somer, Tiina Mattila-Sandholm, Kaisa Poutanen (Corresponding Author)

Research output: Contribution to journalArticleScientificpeer-review

221 Citations (Scopus)

Abstract

Novel oligomers that resist digestion in the upper gut were prepared from oat mixed-linked β-glucan and xylan by enzymatic hydrolysis with lichenase of Bacillus subtilis and xylanase of Trichoderma reesei respectively.
The low-molecular-mass hydrolysis products of β-glucan and xylan were compared with fructooligomers and raffinose in their ability to provide growth substrates for probiotic (Lactobacillus and Bifidobacterium) and intestinal (Bacteroides, Clostridium and Escherichia coli) strains in vitro.
A degradation profile of each carbohydrate and total sugar consumption were analysed with HPLC, and bacterial growth rate with an automatic turbidometer, the Bioscreen C system. β-Glucooligomers and xylooligomers both enhanced the growth of health-promoting probiotic strains as compared with intestinal bacterial growth, but not to a significant level.
Raffinose stimulated the probiotic strains significantly, whereas fructooligomers induced high average growth for intestinal bacteria also.
Original languageEnglish
Pages (from-to)175-181
JournalApplied Microbiology and Biotechnology
Volume49
Issue number2
DOIs
Publication statusPublished - 1998
MoE publication typeA1 Journal article-refereed

Fingerprint

Xylans
Bifidobacterium
Lactobacillus
Probiotics
Raffinose
Growth
Glucans
Hydrolysis
Trichoderma
Bacteroides
Clostridium
Bacillus subtilis
Digestion
High Pressure Liquid Chromatography
Carbohydrates
(1-3)(1-4)-beta-glucan
Escherichia coli
Bacteria
Health

Cite this

Jaskari, Johanna ; Kontula, Pia ; Siitonen, A. ; Jousimies-Somer, Hannele ; Mattila-Sandholm, Tiina ; Poutanen, Kaisa. / Oat beta-glucan and xylan hydrolysates as selective substrates for Bifidobacterium and Lactobacillus strains. In: Applied Microbiology and Biotechnology. 1998 ; Vol. 49, No. 2. pp. 175-181.
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abstract = "Novel oligomers that resist digestion in the upper gut were prepared from oat mixed-linked β-glucan and xylan by enzymatic hydrolysis with lichenase of Bacillus subtilis and xylanase of Trichoderma reesei respectively. The low-molecular-mass hydrolysis products of β-glucan and xylan were compared with fructooligomers and raffinose in their ability to provide growth substrates for probiotic (Lactobacillus and Bifidobacterium) and intestinal (Bacteroides, Clostridium and Escherichia coli) strains in vitro. A degradation profile of each carbohydrate and total sugar consumption were analysed with HPLC, and bacterial growth rate with an automatic turbidometer, the Bioscreen C system. β-Glucooligomers and xylooligomers both enhanced the growth of health-promoting probiotic strains as compared with intestinal bacterial growth, but not to a significant level. Raffinose stimulated the probiotic strains significantly, whereas fructooligomers induced high average growth for intestinal bacteria also.",
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Oat beta-glucan and xylan hydrolysates as selective substrates for Bifidobacterium and Lactobacillus strains. / Jaskari, Johanna; Kontula, Pia; Siitonen, A.; Jousimies-Somer, Hannele; Mattila-Sandholm, Tiina; Poutanen, Kaisa (Corresponding Author).

In: Applied Microbiology and Biotechnology, Vol. 49, No. 2, 1998, p. 175-181.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Oat beta-glucan and xylan hydrolysates as selective substrates for Bifidobacterium and Lactobacillus strains

AU - Jaskari, Johanna

AU - Kontula, Pia

AU - Siitonen, A.

AU - Jousimies-Somer, Hannele

AU - Mattila-Sandholm, Tiina

AU - Poutanen, Kaisa

PY - 1998

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AB - Novel oligomers that resist digestion in the upper gut were prepared from oat mixed-linked β-glucan and xylan by enzymatic hydrolysis with lichenase of Bacillus subtilis and xylanase of Trichoderma reesei respectively. The low-molecular-mass hydrolysis products of β-glucan and xylan were compared with fructooligomers and raffinose in their ability to provide growth substrates for probiotic (Lactobacillus and Bifidobacterium) and intestinal (Bacteroides, Clostridium and Escherichia coli) strains in vitro. A degradation profile of each carbohydrate and total sugar consumption were analysed with HPLC, and bacterial growth rate with an automatic turbidometer, the Bioscreen C system. β-Glucooligomers and xylooligomers both enhanced the growth of health-promoting probiotic strains as compared with intestinal bacterial growth, but not to a significant level. Raffinose stimulated the probiotic strains significantly, whereas fructooligomers induced high average growth for intestinal bacteria also.

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JO - Applied Microbiology and Biotechnology

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SN - 0175-7598

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