Occurrence and molecular characterization of cultivable mesophilic and thermophilic obligate anaerobic bacteria isolated from paper mills

Maija-Liisa Suihko (Corresponding Author), Laila Partanen, Tiina Mattila-Sandholm, Laura Raaska

Research output: Contribution to journalArticleScientificpeer-review

14 Citations (Scopus)

Abstract

The aim of this work was to characterize the cultivable obligate anaerobic bacterial population in paper mill environments. A total of 177 anaerobically grown bacterial isolates were screened for aerotolerance, from which 67 obligate anaerobes were characterized by automated ribotyping and 41 were further identified by partial 16S rDNA sequencing. The mesophilic isolates indicated 11 different taxa (species) within the genus Clostridium and the thermophilic isolates four taxa within the genus Thermoanaerobacterium and one within Thermoanaerobacter (both formerly Clostridium). The most widespread mesophilic bacterium was closely related to C. magnum and occurred in three of four mills. One mill was contaminated with a novel mesophilic bacterium most closely related to C. thiosulfatireducens. The most common thermophile was T. thermosaccharolyticum, occurring in all four mills. The genetic relationships of the mill isolates to described species indicated that most of them are potential members of new species. On the basis of identical ribotypes clay could be identified to be the contamination source of thermophilic bacteria. Automated ribotyping can be a useful tool for the identification of clostridia as soon as comprehensive identification libraries are available.

Original languageEnglish
Pages (from-to)555-561
JournalSystematic and Applied Microbiology
Volume28
Issue number6
DOIs
Publication statusPublished - 2005
MoE publication typeA1 Journal article-refereed

Fingerprint

Ribotyping
pulp and paper mills
Anaerobic Bacteria
Clostridium
DNA fingerprinting
Thermoanaerobacterium
Thermoanaerobacter
mill
thermophilic microorganisms
Bacteria
thermophilic bacteria
ribotypes
bacterium
anaerobes
bacteria
genetic relationships
clay
Ribosomal DNA
Libraries
new species

Keywords

  • Clostridia
  • Thermoanaerobacterium
  • Anaerobes
  • Pulp and paper industry
  • Automated ribotyping

Cite this

Suihko, Maija-Liisa ; Partanen, Laila ; Mattila-Sandholm, Tiina ; Raaska, Laura. / Occurrence and molecular characterization of cultivable mesophilic and thermophilic obligate anaerobic bacteria isolated from paper mills. In: Systematic and Applied Microbiology. 2005 ; Vol. 28, No. 6. pp. 555-561.
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Occurrence and molecular characterization of cultivable mesophilic and thermophilic obligate anaerobic bacteria isolated from paper mills. / Suihko, Maija-Liisa (Corresponding Author); Partanen, Laila; Mattila-Sandholm, Tiina; Raaska, Laura.

In: Systematic and Applied Microbiology, Vol. 28, No. 6, 2005, p. 555-561.

Research output: Contribution to journalArticleScientificpeer-review

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