Oligonucleotide array-in-well platform for detection and genotyping human adenoviruses by utilizing upconverting phosphor label technology

M. Ylihärsilä (Corresponding Author), T. Valta, M. Karp, Liisa Hattara, E. Harju, J. Hölsä, Petri Saviranta, M. Waris, T. Soukka

Research output: Contribution to journalArticleScientificpeer-review

31 Citations (Scopus)

Abstract

We have developed a robust array-in-well test platform based on an oligonucleotide array, combining advantages of simple instrumentation and new upconverting phosphor reporter technology. Upconverting inorganic lanthanide phosphors have a unique property of photoluminescence emission at visible wavelengths under near-infrared excitation. No autofluorescence is produced from the sample or support material, enabling a highly sensitive assay. In this study, the assay is performed in standard 96-well microtiter plates, making the technique easily adaptable to high-throughput analysis. The oligonucleotide array-in-well assay is employed to detect a selection of ten common adenovirus genotypes causing human infections. The study provides a demonstration of the advantages and potential of the upconverting phosphor-based reporter technology in multianalyte assays and anti-Stokes photoluminescence detection with an anti-Stokes photoluminescence imaging device.
Original languageEnglish
Pages (from-to)1456-1461
Number of pages6
JournalAnalytical Chemistry
Volume83
Issue number4
DOIs
Publication statusPublished - 2011
MoE publication typeA1 Journal article-refereed

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Oligonucleotides
Phosphors
Labels
Assays
Photoluminescence
Lanthanoid Series Elements
Demonstrations
Throughput
Infrared radiation
Imaging techniques
Wavelength

Cite this

Ylihärsilä, M. ; Valta, T. ; Karp, M. ; Hattara, Liisa ; Harju, E. ; Hölsä, J. ; Saviranta, Petri ; Waris, M. ; Soukka, T. / Oligonucleotide array-in-well platform for detection and genotyping human adenoviruses by utilizing upconverting phosphor label technology. In: Analytical Chemistry. 2011 ; Vol. 83, No. 4. pp. 1456-1461.
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abstract = "We have developed a robust array-in-well test platform based on an oligonucleotide array, combining advantages of simple instrumentation and new upconverting phosphor reporter technology. Upconverting inorganic lanthanide phosphors have a unique property of photoluminescence emission at visible wavelengths under near-infrared excitation. No autofluorescence is produced from the sample or support material, enabling a highly sensitive assay. In this study, the assay is performed in standard 96-well microtiter plates, making the technique easily adaptable to high-throughput analysis. The oligonucleotide array-in-well assay is employed to detect a selection of ten common adenovirus genotypes causing human infections. The study provides a demonstration of the advantages and potential of the upconverting phosphor-based reporter technology in multianalyte assays and anti-Stokes photoluminescence detection with an anti-Stokes photoluminescence imaging device.",
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Oligonucleotide array-in-well platform for detection and genotyping human adenoviruses by utilizing upconverting phosphor label technology. / Ylihärsilä, M. (Corresponding Author); Valta, T.; Karp, M.; Hattara, Liisa; Harju, E.; Hölsä, J.; Saviranta, Petri; Waris, M.; Soukka, T.

In: Analytical Chemistry, Vol. 83, No. 4, 2011, p. 1456-1461.

Research output: Contribution to journalArticleScientificpeer-review

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AU - Ylihärsilä, M.

AU - Valta, T.

AU - Karp, M.

AU - Hattara, Liisa

AU - Harju, E.

AU - Hölsä, J.

AU - Saviranta, Petri

AU - Waris, M.

AU - Soukka, T.

PY - 2011

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AB - We have developed a robust array-in-well test platform based on an oligonucleotide array, combining advantages of simple instrumentation and new upconverting phosphor reporter technology. Upconverting inorganic lanthanide phosphors have a unique property of photoluminescence emission at visible wavelengths under near-infrared excitation. No autofluorescence is produced from the sample or support material, enabling a highly sensitive assay. In this study, the assay is performed in standard 96-well microtiter plates, making the technique easily adaptable to high-throughput analysis. The oligonucleotide array-in-well assay is employed to detect a selection of ten common adenovirus genotypes causing human infections. The study provides a demonstration of the advantages and potential of the upconverting phosphor-based reporter technology in multianalyte assays and anti-Stokes photoluminescence detection with an anti-Stokes photoluminescence imaging device.

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