On-line capillary electrophoresis instrument for industrial processes

Stella Rovio, Teemu Työppönen, Pertti Vastamäki, Heli Siren, Pekka Savolahti, Ari Hokkanen

Research output: Contribution to conferenceConference AbstractScientific

Abstract

Capillary electrophoresis instrument for on-line control of industrial processes has been developed with continuous flow delivery systems for solvent and sample. The instrument consists of a three partial cell unit, the centre part for the capillary being changeable for fluorescence or UV detections and the front and rear modules with channels for solvent control. The on-line unit has fixed tunnels for dynamic replenishment of electrolyte solution at its both ends. Due to the on-line solvent delivery system constructed into the cell unit, the capillary and the platinum electrodes will be kept uncontaminated. Samples are introduced into the capillary with pressure or electrokinetically or by using a combination of both the techniques. Samples with high ionic strength will be on-line diluted with an electrolyte solution before they are introduced into high-speed CE analyses. Voltages up to 2.5 kV can be used in separations. This presentation demonstrates the work done on the instrumentation and the advantages of dynamic sample and electrolyte feeding. Effect of flow conditions on pressure differences between capillary ends were studied by determining the electroosmotic flows with different electerolyte feeding rates and by comparing migration times and peaks shapes of separated sample peaks from the electropherogram. Electrophoretic separations with laser induced flurescence detection (LIF) are demonstrated by using CAPS as background electrolyte for the analytes fluorescein, mandelic acid and phenyl acetic acid. Preliminary results for the samples oxalate, acetate and sulphate anion, detected by the UV detector at 254 nm wavelength were also performed by using OFM-PDC as a backround electrolyte. On-line applications on pulp and paper process are demonstrated.
Original languageEnglish
Publication statusPublished - 2002
EventPITTCON 2002 : a global technical conference and exposition - New Orleans, United States
Duration: 17 Mar 200222 Mar 2002

Conference

ConferencePITTCON 2002
CountryUnited States
CityNew Orleans
Period17/03/0222/03/02

Fingerprint

Capillary electrophoresis
Electrolytes
Ultraviolet detectors
Oxalates
Platinum
Ionic strength
Fluorescein
Acetic Acid
Sulfates
Pulp
Anions
Tunnels
Acetates
Fluorescence
Wavelength
Electrodes
Lasers
Electric potential

Cite this

Rovio, S., Työppönen, T., Vastamäki, P., Siren, H., Savolahti, P., & Hokkanen, A. (2002). On-line capillary electrophoresis instrument for industrial processes. Abstract from PITTCON 2002 , New Orleans, United States.
Rovio, Stella ; Työppönen, Teemu ; Vastamäki, Pertti ; Siren, Heli ; Savolahti, Pekka ; Hokkanen, Ari. / On-line capillary electrophoresis instrument for industrial processes. Abstract from PITTCON 2002 , New Orleans, United States.
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abstract = "Capillary electrophoresis instrument for on-line control of industrial processes has been developed with continuous flow delivery systems for solvent and sample. The instrument consists of a three partial cell unit, the centre part for the capillary being changeable for fluorescence or UV detections and the front and rear modules with channels for solvent control. The on-line unit has fixed tunnels for dynamic replenishment of electrolyte solution at its both ends. Due to the on-line solvent delivery system constructed into the cell unit, the capillary and the platinum electrodes will be kept uncontaminated. Samples are introduced into the capillary with pressure or electrokinetically or by using a combination of both the techniques. Samples with high ionic strength will be on-line diluted with an electrolyte solution before they are introduced into high-speed CE analyses. Voltages up to 2.5 kV can be used in separations. This presentation demonstrates the work done on the instrumentation and the advantages of dynamic sample and electrolyte feeding. Effect of flow conditions on pressure differences between capillary ends were studied by determining the electroosmotic flows with different electerolyte feeding rates and by comparing migration times and peaks shapes of separated sample peaks from the electropherogram. Electrophoretic separations with laser induced flurescence detection (LIF) are demonstrated by using CAPS as background electrolyte for the analytes fluorescein, mandelic acid and phenyl acetic acid. Preliminary results for the samples oxalate, acetate and sulphate anion, detected by the UV detector at 254 nm wavelength were also performed by using OFM-PDC as a backround electrolyte. On-line applications on pulp and paper process are demonstrated.",
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Rovio, S, Työppönen, T, Vastamäki, P, Siren, H, Savolahti, P & Hokkanen, A 2002, 'On-line capillary electrophoresis instrument for industrial processes' PITTCON 2002 , New Orleans, United States, 17/03/02 - 22/03/02, .

On-line capillary electrophoresis instrument for industrial processes. / Rovio, Stella; Työppönen, Teemu; Vastamäki, Pertti; Siren, Heli; Savolahti, Pekka; Hokkanen, Ari.

2002. Abstract from PITTCON 2002 , New Orleans, United States.

Research output: Contribution to conferenceConference AbstractScientific

TY - CONF

T1 - On-line capillary electrophoresis instrument for industrial processes

AU - Rovio, Stella

AU - Työppönen, Teemu

AU - Vastamäki, Pertti

AU - Siren, Heli

AU - Savolahti, Pekka

AU - Hokkanen, Ari

PY - 2002

Y1 - 2002

N2 - Capillary electrophoresis instrument for on-line control of industrial processes has been developed with continuous flow delivery systems for solvent and sample. The instrument consists of a three partial cell unit, the centre part for the capillary being changeable for fluorescence or UV detections and the front and rear modules with channels for solvent control. The on-line unit has fixed tunnels for dynamic replenishment of electrolyte solution at its both ends. Due to the on-line solvent delivery system constructed into the cell unit, the capillary and the platinum electrodes will be kept uncontaminated. Samples are introduced into the capillary with pressure or electrokinetically or by using a combination of both the techniques. Samples with high ionic strength will be on-line diluted with an electrolyte solution before they are introduced into high-speed CE analyses. Voltages up to 2.5 kV can be used in separations. This presentation demonstrates the work done on the instrumentation and the advantages of dynamic sample and electrolyte feeding. Effect of flow conditions on pressure differences between capillary ends were studied by determining the electroosmotic flows with different electerolyte feeding rates and by comparing migration times and peaks shapes of separated sample peaks from the electropherogram. Electrophoretic separations with laser induced flurescence detection (LIF) are demonstrated by using CAPS as background electrolyte for the analytes fluorescein, mandelic acid and phenyl acetic acid. Preliminary results for the samples oxalate, acetate and sulphate anion, detected by the UV detector at 254 nm wavelength were also performed by using OFM-PDC as a backround electrolyte. On-line applications on pulp and paper process are demonstrated.

AB - Capillary electrophoresis instrument for on-line control of industrial processes has been developed with continuous flow delivery systems for solvent and sample. The instrument consists of a three partial cell unit, the centre part for the capillary being changeable for fluorescence or UV detections and the front and rear modules with channels for solvent control. The on-line unit has fixed tunnels for dynamic replenishment of electrolyte solution at its both ends. Due to the on-line solvent delivery system constructed into the cell unit, the capillary and the platinum electrodes will be kept uncontaminated. Samples are introduced into the capillary with pressure or electrokinetically or by using a combination of both the techniques. Samples with high ionic strength will be on-line diluted with an electrolyte solution before they are introduced into high-speed CE analyses. Voltages up to 2.5 kV can be used in separations. This presentation demonstrates the work done on the instrumentation and the advantages of dynamic sample and electrolyte feeding. Effect of flow conditions on pressure differences between capillary ends were studied by determining the electroosmotic flows with different electerolyte feeding rates and by comparing migration times and peaks shapes of separated sample peaks from the electropherogram. Electrophoretic separations with laser induced flurescence detection (LIF) are demonstrated by using CAPS as background electrolyte for the analytes fluorescein, mandelic acid and phenyl acetic acid. Preliminary results for the samples oxalate, acetate and sulphate anion, detected by the UV detector at 254 nm wavelength were also performed by using OFM-PDC as a backround electrolyte. On-line applications on pulp and paper process are demonstrated.

M3 - Conference Abstract

ER -

Rovio S, Työppönen T, Vastamäki P, Siren H, Savolahti P, Hokkanen A. On-line capillary electrophoresis instrument for industrial processes. 2002. Abstract from PITTCON 2002 , New Orleans, United States.