Online capillary electrophoresis for monitoring carboxylic acid production by yeast during bioreactor cultivations

Heidi Turkia (Corresponding Author), Sami Holmström, Toni Paasikallio, Heli Sirén, Merja Penttilä, Juha Pekka Pitkänen

    Research output: Contribution to journalArticleScientificpeer-review

    15 Citations (Scopus)

    Abstract

    Bioprocess monitoring can improve the understanding and control of biotechnological processes. When analyses are carried out as automated online measurements, manual steps of the analysis procedures are avoided, thus decreasing both the time required for analyses and systematic errors. In this study, an online capillary electrophoresis (CE) system with flow-through sample vial made in-house and action control programming was assembled to monitor carboxylic acid production by Kluyveromyces lactis and Saccharomyces cerevisiae during two different bioreactor cultivations. The relative standard deviations were less than 0.6% for intraday migration times and the total analysis time was less than 20 min. The system operated continuously and automatically up to 6 days and produced data concerning carboxylic acid production during the cultivations. The successful test runs demonstrated that this system has potential for the monitoring of biotechnological processes.

    Original languageEnglish
    Pages (from-to)9705-9712
    JournalAnalytical Chemistry
    Volume85
    Issue number20
    DOIs
    Publication statusPublished - 15 Oct 2013
    MoE publication typeA1 Journal article-refereed

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