Optical design and development of a cost-efficient, compact, super-sensitive scanning confocal fluorescence microscope

Yunfeng Nie, Sanna Aikio, Annukka Kokkonen, Sanna Uusitalo, Teemu Sipola, Simonetta Grilli, Heidi Ottevaere

Research output: Chapter in Book/Report/Conference proceedingConference article in proceedingsScientificpeer-review

1 Citation (Scopus)

Abstract

The effective detection of very low abundant biomarkers can enable fast diagnosis of many severe and disabling diseases (e.g. Alzheimer’s Disease) at an early stage. To develop a cost-efficient, super-sensitive optical fluorescence detection microscope, we have proposed an optical modelling approach to predict the signal-noise-ratio that considers various noise sources introduced by the components of the detection system. After the optimal design is identified, a tolerance analysis regarding typical perturbations is performed for further mechanical design and assembly. Finally, experiments have demonstrated a limit of detection at a low abundant concentration reaching 0.05 pmol/ml.
Original languageEnglish
Title of host publicationOptical Methods for Inspection, Characterization, and Imaging of Biomaterials V
EditorsPietro Ferraro, Simonetta Grilli, Monika Ritsch-Marte, Christoph K. Hitzenberger
PublisherInternational Society for Optics and Photonics SPIE
ISBN (Electronic)978-1-5106-4406-9
DOIs
Publication statusPublished - 20 Jun 2021
MoE publication typeA4 Article in a conference publication
EventOptical Methods for Inspection, Characterization, and Imaging of Biomaterials V 2021 - Virtual, Online, Germany
Duration: 21 Jun 202125 Jun 2021

Publication series

SeriesProceedings of SPIE
Volume11786
ISSN0277-786X

Conference

ConferenceOptical Methods for Inspection, Characterization, and Imaging of Biomaterials V 2021
Country/TerritoryGermany
CityVirtual, Online
Period21/06/2125/06/21

Funding

The authors acknowledge the EU funding within the Horizon 2020 Program, under the FET-OPEN Project “SensApp”, Grant Agreement No.829104. This work was also supported in part by FWO (research project G044516N), the Methusalem and Hercules foundations and the OZR of the Vrije Universiteit Brussel (VUB).

Keywords

  • Alzheimer's disease
  • Confocal scanning
  • Fluorescence microscopy
  • Laser induced fluorescence
  • Optical design
  • Optical scatter modelling
  • Tolerance analysis

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