Optical design and development of a cost-efficient, compact, supersensitive scanning confocal fluorescence microscope

Yunfeng Nie, Aikio Sanna, Annukka Kokkonen, Uusitalo Sanna, Teemu Sipola, Simonetta Grilli, Heidi Ottevaere

Research output: Chapter in Book/Report/Conference proceedingConference article in proceedingsScientificpeer-review

Abstract

The effective detection of very low abundant biomarkers can enable fast diagnosis of many severe and disabling diseases (e.g. Alzheimer's Disease) at an early stage. To develop a cost-efficient, super-sensitive optical fluorescence detection microscope, we have proposed an optical modelling approach to predict the signal-noise-ratio that considers various noise sources introduced by the components of the detection system. After the optimal design is identified, a tolerance analysis regarding typical perturbations is performed for further mechanical design and assembly. Finally, experiments have demonstrated a limit of detection at a low abundant concentration reaching 0.05 pmol/ml.

Original languageEnglish
Title of host publicationOptical Methods for Inspection, Characterization, and Imaging of Biomaterials V
EditorsPietro Ferraro, Simonetta Grilli, Monika Ritsch-Marte, Christoph K. Hitzenberger
PublisherInternational Society for Optics and Photonics SPIE
ISBN (Electronic)978-1-5106-4406-9
DOIs
Publication statusPublished - 2021
MoE publication typeA4 Article in a conference publication
EventOptical Methods for Inspection, Characterization, and Imaging of Biomaterials V 2021 - Virtual, Online, Germany
Duration: 21 Jun 202125 Jun 2021

Publication series

SeriesProceedings of SPIE - The International Society for Optical Engineering
Volume11786
ISSN0277-786X

Conference

ConferenceOptical Methods for Inspection, Characterization, and Imaging of Biomaterials V 2021
Country/TerritoryGermany
CityVirtual, Online
Period21/06/2125/06/21

Keywords

  • Alzheimer's disease
  • Confocal scanning
  • Fluorescence microscopy
  • Laser induced fluorescence
  • Optical design
  • Optical scatter modelling
  • Tolerance analysis

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