Optical design and development of a cost-efficient, compact, super-sensitive scanning confocal fluorescence microscope

Yunfeng Nie; Sanna Aikio; Annukka Kokkonen; Sanna Uusitalo; Teemu Sipola; Simonetta Grilli; Heidi Ottevaere

doi:10.1117/12.2593354

Optical design and development of a cost-efficient, compact, super-sensitive scanning confocal fluorescence microscope

Yunfeng Nie
, Sanna Aikio
, Annukka Kokkonen
, Sanna Uusitalo
, Teemu Sipola
, Simonetta Grilli
, Heidi Ottevaere

Vrije Universiteit Brussel
Institute of Applied Sciences and Intelligent Systems (ISASI-CNR)

Research output: Chapter in Book/Report/Conference proceeding › Conference article in proceedings › Scientific › peer-review

1 Citation (Scopus)

Abstract

The effective detection of very low abundant biomarkers can enable fast diagnosis of many severe and disabling diseases (e.g. Alzheimer’s Disease) at an early stage. To develop a cost-efficient, super-sensitive optical fluorescence detection microscope, we have proposed an optical modelling approach to predict the signal-noise-ratio that considers various noise sources introduced by the components of the detection system. After the optimal design is identified, a tolerance analysis regarding typical perturbations is performed for further mechanical design and assembly. Finally, experiments have demonstrated a limit of detection at a low abundant concentration reaching 0.05 pmol/ml.

Original language	English
Title of host publication	Optical Methods for Inspection, Characterization, and Imaging of Biomaterials V
Editors	Pietro Ferraro, Simonetta Grilli, Monika Ritsch-Marte, Christoph K. Hitzenberger
Publisher	International Society for Optics and Photonics SPIE
ISBN (Electronic)	978-1-5106-4406-9
DOIs	https://doi.org/10.1117/12.2593354
Publication status	Published - 20 Jun 2021
MoE publication type	A4 Article in a conference publication
Event	Optical Methods for Inspection, Characterization, and Imaging of Biomaterials V 2021 - Virtual, Online, Germany Duration: 21 Jun 2021 → 25 Jun 2021

Publication series

Series	Proceedings of SPIE
Volume	11786
ISSN	0277-786X

Conference

Conference	Optical Methods for Inspection, Characterization, and Imaging of Biomaterials V 2021
Country/Territory	Germany
City	Virtual, Online
Period	21/06/21 → 25/06/21

Funding

The authors acknowledge the EU funding within the Horizon 2020 Program, under the FET-OPEN Project “SensApp”, Grant Agreement No.829104. This work was also supported in part by FWO (research project G044516N), the Methusalem and Hercules foundations and the OZR of the Vrije Universiteit Brussel (VUB).

Keywords

Alzheimer's disease
Confocal scanning
Fluorescence microscopy
Laser induced fluorescence
Optical design
Optical scatter modelling
Tolerance analysis

Access to Document

10.1117/12.2593354

Cite this

Nie, Y., Aikio, S., Kokkonen, A., Uusitalo, S., Sipola, T., Grilli, S., & Ottevaere, H. (2021). Optical design and development of a cost-efficient, compact, super-sensitive scanning confocal fluorescence microscope. In P. Ferraro, S. Grilli, M. Ritsch-Marte, & C. K. Hitzenberger (Eds.), Optical Methods for Inspection, Characterization, and Imaging of Biomaterials V Article 117861E International Society for Optics and Photonics SPIE. https://doi.org/10.1117/12.2593354

Nie, Yunfeng ; Aikio, Sanna ; Kokkonen, Annukka et al. / Optical design and development of a cost-efficient, compact, super-sensitive scanning confocal fluorescence microscope. Optical Methods for Inspection, Characterization, and Imaging of Biomaterials V. editor / Pietro Ferraro ; Simonetta Grilli ; Monika Ritsch-Marte ; Christoph K. Hitzenberger. International Society for Optics and Photonics SPIE, 2021. (Proceedings of SPIE, Vol. 11786).

@inproceedings{e5f8c8d5feb447a4adeafb7a7ec0db2e,

title = "Optical design and development of a cost-efficient, compact, super-sensitive scanning confocal fluorescence microscope",

abstract = "The effective detection of very low abundant biomarkers can enable fast diagnosis of many severe and disabling diseases (e.g. Alzheimer{\textquoteright}s Disease) at an early stage. To develop a cost-efficient, super-sensitive optical fluorescence detection microscope, we have proposed an optical modelling approach to predict the signal-noise-ratio that considers various noise sources introduced by the components of the detection system. After the optimal design is identified, a tolerance analysis regarding typical perturbations is performed for further mechanical design and assembly. Finally, experiments have demonstrated a limit of detection at a low abundant concentration reaching 0.05 pmol/ml.",

keywords = "Alzheimer's disease, Confocal scanning, Fluorescence microscopy, Laser induced fluorescence, Optical design, Optical scatter modelling, Tolerance analysis",

author = "Yunfeng Nie and Sanna Aikio and Annukka Kokkonen and Sanna Uusitalo and Teemu Sipola and Simonetta Grilli and Heidi Ottevaere",

note = "Funding Information: The authors acknowledge the EU funding within the Horizon 2020 Program, under the FET-OPEN Project “SensApp”, Grant Agreement No.829104. This work was also supported in part by FWO (research project G044516N), the Methusalem and Hercules foundations and the OZR of the Vrije Universiteit Brussel (VUB).; Optical Methods for Inspection, Characterization, and Imaging of Biomaterials V 2021 ; Conference date: 21-06-2021 Through 25-06-2021",

year = "2021",

month = jun,

day = "20",

doi = "10.1117/12.2593354",

language = "English",

series = "Proceedings of SPIE",

publisher = "International Society for Optics and Photonics SPIE",

editor = "Pietro Ferraro and Simonetta Grilli and Monika Ritsch-Marte and Hitzenberger, \{Christoph K.\}",

booktitle = "Optical Methods for Inspection, Characterization, and Imaging of Biomaterials V",

address = "United States",

}

Nie, Y, Aikio, S , Kokkonen, A, Uusitalo, S, Sipola, T, Grilli, S & Ottevaere, H 2021, Optical design and development of a cost-efficient, compact, super-sensitive scanning confocal fluorescence microscope. in P Ferraro, S Grilli, M Ritsch-Marte & CK Hitzenberger (eds), Optical Methods for Inspection, Characterization, and Imaging of Biomaterials V., 117861E, International Society for Optics and Photonics SPIE, Proceedings of SPIE, vol. 11786, Optical Methods for Inspection, Characterization, and Imaging of Biomaterials V 2021, Virtual, Online, Germany, 21/06/21. https://doi.org/10.1117/12.2593354

Optical design and development of a cost-efficient, compact, super-sensitive scanning confocal fluorescence microscope. / Nie, Yunfeng; Aikio, Sanna ; Kokkonen, Annukka et al.
Optical Methods for Inspection, Characterization, and Imaging of Biomaterials V. ed. / Pietro Ferraro; Simonetta Grilli; Monika Ritsch-Marte; Christoph K. Hitzenberger. International Society for Optics and Photonics SPIE, 2021. 117861E (Proceedings of SPIE, Vol. 11786).

Research output: Chapter in Book/Report/Conference proceeding › Conference article in proceedings › Scientific › peer-review

TY - GEN

T1 - Optical design and development of a cost-efficient, compact, super-sensitive scanning confocal fluorescence microscope

AU - Nie, Yunfeng

AU - Aikio, Sanna

AU - Kokkonen, Annukka

AU - Uusitalo, Sanna

AU - Sipola, Teemu

AU - Grilli, Simonetta

AU - Ottevaere, Heidi

N1 - Funding Information: The authors acknowledge the EU funding within the Horizon 2020 Program, under the FET-OPEN Project “SensApp”, Grant Agreement No.829104. This work was also supported in part by FWO (research project G044516N), the Methusalem and Hercules foundations and the OZR of the Vrije Universiteit Brussel (VUB).

PY - 2021/6/20

Y1 - 2021/6/20

N2 - The effective detection of very low abundant biomarkers can enable fast diagnosis of many severe and disabling diseases (e.g. Alzheimer’s Disease) at an early stage. To develop a cost-efficient, super-sensitive optical fluorescence detection microscope, we have proposed an optical modelling approach to predict the signal-noise-ratio that considers various noise sources introduced by the components of the detection system. After the optimal design is identified, a tolerance analysis regarding typical perturbations is performed for further mechanical design and assembly. Finally, experiments have demonstrated a limit of detection at a low abundant concentration reaching 0.05 pmol/ml.

AB - The effective detection of very low abundant biomarkers can enable fast diagnosis of many severe and disabling diseases (e.g. Alzheimer’s Disease) at an early stage. To develop a cost-efficient, super-sensitive optical fluorescence detection microscope, we have proposed an optical modelling approach to predict the signal-noise-ratio that considers various noise sources introduced by the components of the detection system. After the optimal design is identified, a tolerance analysis regarding typical perturbations is performed for further mechanical design and assembly. Finally, experiments have demonstrated a limit of detection at a low abundant concentration reaching 0.05 pmol/ml.

KW - Alzheimer's disease

KW - Confocal scanning

KW - Fluorescence microscopy

KW - Laser induced fluorescence

KW - Optical design

KW - Optical scatter modelling

KW - Tolerance analysis

UR - https://www.scopus.com/pages/publications/85109610349

U2 - 10.1117/12.2593354

DO - 10.1117/12.2593354

M3 - Conference article in proceedings

AN - SCOPUS:85109610349

T3 - Proceedings of SPIE

BT - Optical Methods for Inspection, Characterization, and Imaging of Biomaterials V

A2 - Ferraro, Pietro

A2 - Grilli, Simonetta

A2 - Ritsch-Marte, Monika

A2 - Hitzenberger, Christoph K.

PB - International Society for Optics and Photonics SPIE

T2 - Optical Methods for Inspection, Characterization, and Imaging of Biomaterials V 2021

Y2 - 21 June 2021 through 25 June 2021

ER -

Nie Y, Aikio S , Kokkonen A, Uusitalo S, Sipola T, Grilli S et al. Optical design and development of a cost-efficient, compact, super-sensitive scanning confocal fluorescence microscope. In Ferraro P, Grilli S, Ritsch-Marte M, Hitzenberger CK, editors, Optical Methods for Inspection, Characterization, and Imaging of Biomaterials V. International Society for Optics and Photonics SPIE. 2021. 117861E. (Proceedings of SPIE, Vol. 11786). doi: 10.1117/12.2593354

Optical design and development of a cost-efficient, compact, super-sensitive scanning confocal fluorescence microscope

Abstract

Publication series

Conference

Funding

Keywords

Access to Document

Other files and links

Fingerprint

SensApp: Super-sensitive detection of Alzheimer’s disease biomarkers in plasma by an innovative droplet split-and-stack approach

Cite this

Optical design and development of a cost-efficient, compact, super-sensitive scanning confocal fluorescence microscope

Abstract

Publication series

Conference

Funding

Keywords

Access to Document

Other files and links

Fingerprint

Projects

SensApp: Super-sensitive detection of Alzheimer’s disease biomarkers in plasma by an innovative droplet split-and-stack approach

Cite this