Optimization of capillary electrophoretic‐electrospray ionization‐mass spectrometric analysis of catecholamines

Katariina Vuorensola, Juha Kokkonen, Heli Siren (Corresponding Author), Raimo Ketola

Research output: Contribution to journalArticleScientificpeer-review

69 Citations (Scopus)

Abstract

The capillary electrophoretic‐mass spectrometric analysis (CE‐MS) of catecholamines was optimized with coaxial sheath flow interface and electrospray ionization (ESI). The parameters studied included the sheath liquid composition and its flow rate, separation conditions in ammonium acetate buffer together with the ESI and cone voltages as mass spectrometric parameters. In addition, the effect of ESI voltage on injection as well as the siphoning effect were considered. The optimized conditions were a sheath liquid composition of methanol‐water (80:20 v/v) with 0.5% acetic acid, with a flow rate of 6 νL/min. The capillary electrophoretic separation parameters were optimized with 50 mM ammonium acetate buffer, pH 4.0, to +25 kV separation voltage together with a pressure of 0.1 psi. The most intensive signals were obtained with an ESI voltage of +4.0 kV and a cone voltage of +20 V. The nonactive ESI voltage during injection as well as avoidance of the siphoning effect increased the sensitivity of the MS detection considerably. The use of ammonium hydroxide as the CE capillary conditioning solution instead of sodium hydroxide did not affect the CE‐MS performance, but allowed the conditioning of the capillary between analyses to be performed in the MS without contaminating the ion source.
Original languageEnglish
Pages (from-to)4347-4354
Number of pages8
JournalElectrophoresis
Volume22
Issue number20
DOIs
Publication statusPublished - 2001
MoE publication typeA1 Journal article-refereed

Fingerprint

Electrospray ionization
Catecholamines
Electric potential
Buffers
Ammonium Hydroxide
Cones
Sodium Hydroxide
Flow rate
Injections
Acetic Acid
Liquids
Ion sources
Chemical analysis
Ions
Pressure
ammonium acetate

Cite this

Vuorensola, Katariina ; Kokkonen, Juha ; Siren, Heli ; Ketola, Raimo. / Optimization of capillary electrophoretic‐electrospray ionization‐mass spectrometric analysis of catecholamines. In: Electrophoresis. 2001 ; Vol. 22, No. 20. pp. 4347-4354.
@article{13c4d1301ced472cb34f612264430879,
title = "Optimization of capillary electrophoretic‐electrospray ionization‐mass spectrometric analysis of catecholamines",
abstract = "The capillary electrophoretic‐mass spectrometric analysis (CE‐MS) of catecholamines was optimized with coaxial sheath flow interface and electrospray ionization (ESI). The parameters studied included the sheath liquid composition and its flow rate, separation conditions in ammonium acetate buffer together with the ESI and cone voltages as mass spectrometric parameters. In addition, the effect of ESI voltage on injection as well as the siphoning effect were considered. The optimized conditions were a sheath liquid composition of methanol‐water (80:20 v/v) with 0.5{\%} acetic acid, with a flow rate of 6 νL/min. The capillary electrophoretic separation parameters were optimized with 50 mM ammonium acetate buffer, pH 4.0, to +25 kV separation voltage together with a pressure of 0.1 psi. The most intensive signals were obtained with an ESI voltage of +4.0 kV and a cone voltage of +20 V. The nonactive ESI voltage during injection as well as avoidance of the siphoning effect increased the sensitivity of the MS detection considerably. The use of ammonium hydroxide as the CE capillary conditioning solution instead of sodium hydroxide did not affect the CE‐MS performance, but allowed the conditioning of the capillary between analyses to be performed in the MS without contaminating the ion source.",
author = "Katariina Vuorensola and Juha Kokkonen and Heli Siren and Raimo Ketola",
year = "2001",
doi = "10.1002/1522-2683(200112)22:20<4347::AID-ELPS4347>3.0.CO;2-J",
language = "English",
volume = "22",
pages = "4347--4354",
journal = "Electrophoresis",
issn = "0173-0835",
publisher = "Wiley-VCH Verlag",
number = "20",

}

Optimization of capillary electrophoretic‐electrospray ionization‐mass spectrometric analysis of catecholamines. / Vuorensola, Katariina; Kokkonen, Juha; Siren, Heli (Corresponding Author); Ketola, Raimo.

In: Electrophoresis, Vol. 22, No. 20, 2001, p. 4347-4354.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Optimization of capillary electrophoretic‐electrospray ionization‐mass spectrometric analysis of catecholamines

AU - Vuorensola, Katariina

AU - Kokkonen, Juha

AU - Siren, Heli

AU - Ketola, Raimo

PY - 2001

Y1 - 2001

N2 - The capillary electrophoretic‐mass spectrometric analysis (CE‐MS) of catecholamines was optimized with coaxial sheath flow interface and electrospray ionization (ESI). The parameters studied included the sheath liquid composition and its flow rate, separation conditions in ammonium acetate buffer together with the ESI and cone voltages as mass spectrometric parameters. In addition, the effect of ESI voltage on injection as well as the siphoning effect were considered. The optimized conditions were a sheath liquid composition of methanol‐water (80:20 v/v) with 0.5% acetic acid, with a flow rate of 6 νL/min. The capillary electrophoretic separation parameters were optimized with 50 mM ammonium acetate buffer, pH 4.0, to +25 kV separation voltage together with a pressure of 0.1 psi. The most intensive signals were obtained with an ESI voltage of +4.0 kV and a cone voltage of +20 V. The nonactive ESI voltage during injection as well as avoidance of the siphoning effect increased the sensitivity of the MS detection considerably. The use of ammonium hydroxide as the CE capillary conditioning solution instead of sodium hydroxide did not affect the CE‐MS performance, but allowed the conditioning of the capillary between analyses to be performed in the MS without contaminating the ion source.

AB - The capillary electrophoretic‐mass spectrometric analysis (CE‐MS) of catecholamines was optimized with coaxial sheath flow interface and electrospray ionization (ESI). The parameters studied included the sheath liquid composition and its flow rate, separation conditions in ammonium acetate buffer together with the ESI and cone voltages as mass spectrometric parameters. In addition, the effect of ESI voltage on injection as well as the siphoning effect were considered. The optimized conditions were a sheath liquid composition of methanol‐water (80:20 v/v) with 0.5% acetic acid, with a flow rate of 6 νL/min. The capillary electrophoretic separation parameters were optimized with 50 mM ammonium acetate buffer, pH 4.0, to +25 kV separation voltage together with a pressure of 0.1 psi. The most intensive signals were obtained with an ESI voltage of +4.0 kV and a cone voltage of +20 V. The nonactive ESI voltage during injection as well as avoidance of the siphoning effect increased the sensitivity of the MS detection considerably. The use of ammonium hydroxide as the CE capillary conditioning solution instead of sodium hydroxide did not affect the CE‐MS performance, but allowed the conditioning of the capillary between analyses to be performed in the MS without contaminating the ion source.

U2 - 10.1002/1522-2683(200112)22:20<4347::AID-ELPS4347>3.0.CO;2-J

DO - 10.1002/1522-2683(200112)22:20<4347::AID-ELPS4347>3.0.CO;2-J

M3 - Article

VL - 22

SP - 4347

EP - 4354

JO - Electrophoresis

JF - Electrophoresis

SN - 0173-0835

IS - 20

ER -