Optimization of Isomaltooligosaccharide Size Distribution by Acceptor Reaction of Weissella confusa Dextransucrase and Characterization of Novel [alpha]-(1->2)-Branched Isomaltooligosaccharides

Qiao Shi (Corresponding Author), Yaxi Hou, Minna Juvonen, Päivi Tuomainen, Ilkka Kajala, Shraddha Shukla, Arun Goyal, Hannu Maaheimo, Kati Katina, Maija Tenkanen

    Research output: Contribution to journalArticleScientificpeer-review

    9 Citations (Scopus)

    Abstract

    Long-chain isomaltooligosaccharides (IMOs) are promising prebiotics. IMOs were produced by a Weissella confusa dextransucrase via maltose acceptor reaction. The inputs of substrates (i.e., sucrose and maltose, 0.15–1 M) and dextransucrase (1–10 U/g sucrose) were used to control IMO yield and profile. According to response surface modeling, 1 M sucrose and 0.5 M maltose were optimal for the synthesis of longer IMOs, whereas the dextransucrase dosage showed no significant effect. In addition to the principal linear IMOs, a homologous series of minor IMOs were also produced from maltose. As identified by MSn and NMR spectroscopy, the minor trisaccharide contained an α-(1→2)-linked glucosyl residue on the reducing residue of maltose and thus was α-d-glucopyranosyl-(1→2)-[α-d-glucopyranosyl-(1→4)]-d-glucopyranose (centose). The higher members of the series were probably formed by the attachment of a single unit branch to linear IMOs. This is the first report of such α-(1→2)-branched IMOs produced from maltose by a dextransucrase.
    Original languageEnglish
    Pages (from-to)3276-3286
    JournalJournal of Agricultural and Food Chemistry
    Volume64
    Issue number16
    DOIs
    Publication statusPublished - 2016
    MoE publication typeA1 Journal article-refereed

    Keywords

    • isomaltooligosaccharides
    • dextransucrase
    • maltose acceptor reaction
    • response surface modeling
    • [alpha]-(1->2)-linkage
    • centose

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