Optimization of Isomaltooligosaccharide Size Distribution by Acceptor Reaction of Weissella confusa Dextransucrase and Characterization of Novel [alpha]-(1->2)-Branched Isomaltooligosaccharides

Qiao Shi (Corresponding Author), Yaxi Hou, Minna Juvonen, Päivi Tuomainen, Ilkka Kajala, Shraddha Shukla, Arun Goyal, Hannu Maaheimo, Kati Katina, Maija Tenkanen

Research output: Contribution to journalArticleScientificpeer-review

6 Citations (Scopus)

Abstract

Long-chain isomaltooligosaccharides (IMOs) are promising prebiotics. IMOs were produced by a Weissella confusa dextransucrase via maltose acceptor reaction. The inputs of substrates (i.e., sucrose and maltose, 0.15–1 M) and dextransucrase (1–10 U/g sucrose) were used to control IMO yield and profile. According to response surface modeling, 1 M sucrose and 0.5 M maltose were optimal for the synthesis of longer IMOs, whereas the dextransucrase dosage showed no significant effect. In addition to the principal linear IMOs, a homologous series of minor IMOs were also produced from maltose. As identified by MSn and NMR spectroscopy, the minor trisaccharide contained an α-(1→2)-linked glucosyl residue on the reducing residue of maltose and thus was α-d-glucopyranosyl-(1→2)-[α-d-glucopyranosyl-(1→4)]-d-glucopyranose (centose). The higher members of the series were probably formed by the attachment of a single unit branch to linear IMOs. This is the first report of such α-(1→2)-branched IMOs produced from maltose by a dextransucrase.
Original languageEnglish
Pages (from-to)3276-3286
JournalJournal of Agricultural and Food Chemistry
Volume64
Issue number16
DOIs
Publication statusPublished - 2016
MoE publication typeA1 Journal article-refereed

Fingerprint

dextransucrase
Weissella
Weissella confusa
Maltose
maltose
Sucrose
sucrose
trisaccharides
Trisaccharides
Prebiotics
prebiotics
Nuclear magnetic resonance spectroscopy
nuclear magnetic resonance spectroscopy
Magnetic Resonance Spectroscopy
synthesis
Substrates
dosage

Keywords

  • isomaltooligosaccharides
  • dextransucrase
  • maltose acceptor reaction
  • response surface modeling
  • [alpha]-(1->2)-linkage
  • centose

Cite this

Shi, Qiao ; Hou, Yaxi ; Juvonen, Minna ; Tuomainen, Päivi ; Kajala, Ilkka ; Shukla, Shraddha ; Goyal, Arun ; Maaheimo, Hannu ; Katina, Kati ; Tenkanen, Maija. / Optimization of Isomaltooligosaccharide Size Distribution by Acceptor Reaction of Weissella confusa Dextransucrase and Characterization of Novel [alpha]-(1->2)-Branched Isomaltooligosaccharides. In: Journal of Agricultural and Food Chemistry. 2016 ; Vol. 64, No. 16. pp. 3276-3286.
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author = "Qiao Shi and Yaxi Hou and Minna Juvonen and P{\"a}ivi Tuomainen and Ilkka Kajala and Shraddha Shukla and Arun Goyal and Hannu Maaheimo and Kati Katina and Maija Tenkanen",
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Optimization of Isomaltooligosaccharide Size Distribution by Acceptor Reaction of Weissella confusa Dextransucrase and Characterization of Novel [alpha]-(1->2)-Branched Isomaltooligosaccharides. / Shi, Qiao (Corresponding Author); Hou, Yaxi; Juvonen, Minna; Tuomainen, Päivi; Kajala, Ilkka; Shukla, Shraddha; Goyal, Arun; Maaheimo, Hannu; Katina, Kati; Tenkanen, Maija.

In: Journal of Agricultural and Food Chemistry, Vol. 64, No. 16, 2016, p. 3276-3286.

Research output: Contribution to journalArticleScientificpeer-review

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T1 - Optimization of Isomaltooligosaccharide Size Distribution by Acceptor Reaction of Weissella confusa Dextransucrase and Characterization of Novel [alpha]-(1->2)-Branched Isomaltooligosaccharides

AU - Shi, Qiao

AU - Hou, Yaxi

AU - Juvonen, Minna

AU - Tuomainen, Päivi

AU - Kajala, Ilkka

AU - Shukla, Shraddha

AU - Goyal, Arun

AU - Maaheimo, Hannu

AU - Katina, Kati

AU - Tenkanen, Maija

PY - 2016

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N2 - Long-chain isomaltooligosaccharides (IMOs) are promising prebiotics. IMOs were produced by a Weissella confusa dextransucrase via maltose acceptor reaction. The inputs of substrates (i.e., sucrose and maltose, 0.15–1 M) and dextransucrase (1–10 U/g sucrose) were used to control IMO yield and profile. According to response surface modeling, 1 M sucrose and 0.5 M maltose were optimal for the synthesis of longer IMOs, whereas the dextransucrase dosage showed no significant effect. In addition to the principal linear IMOs, a homologous series of minor IMOs were also produced from maltose. As identified by MSn and NMR spectroscopy, the minor trisaccharide contained an α-(1→2)-linked glucosyl residue on the reducing residue of maltose and thus was α-d-glucopyranosyl-(1→2)-[α-d-glucopyranosyl-(1→4)]-d-glucopyranose (centose). The higher members of the series were probably formed by the attachment of a single unit branch to linear IMOs. This is the first report of such α-(1→2)-branched IMOs produced from maltose by a dextransucrase.

AB - Long-chain isomaltooligosaccharides (IMOs) are promising prebiotics. IMOs were produced by a Weissella confusa dextransucrase via maltose acceptor reaction. The inputs of substrates (i.e., sucrose and maltose, 0.15–1 M) and dextransucrase (1–10 U/g sucrose) were used to control IMO yield and profile. According to response surface modeling, 1 M sucrose and 0.5 M maltose were optimal for the synthesis of longer IMOs, whereas the dextransucrase dosage showed no significant effect. In addition to the principal linear IMOs, a homologous series of minor IMOs were also produced from maltose. As identified by MSn and NMR spectroscopy, the minor trisaccharide contained an α-(1→2)-linked glucosyl residue on the reducing residue of maltose and thus was α-d-glucopyranosyl-(1→2)-[α-d-glucopyranosyl-(1→4)]-d-glucopyranose (centose). The higher members of the series were probably formed by the attachment of a single unit branch to linear IMOs. This is the first report of such α-(1→2)-branched IMOs produced from maltose by a dextransucrase.

KW - isomaltooligosaccharides

KW - dextransucrase

KW - maltose acceptor reaction

KW - response surface modeling

KW - [alpha]-(1->2)-linkage

KW - centose

U2 - 10.1021/acs.jafc.6b01356

DO - 10.1021/acs.jafc.6b01356

M3 - Article

VL - 64

SP - 3276

EP - 3286

JO - Journal of Agricultural and Food Chemistry

JF - Journal of Agricultural and Food Chemistry

SN - 0021-8561

IS - 16

ER -