Optimization of media nitrogen and copper concentrations for regeneration of green plants from polyembryogenic cultures of barley (Hordeum vulgare L.)

Anna-Maria Nuutila (Corresponding Author), Jari Hämäläinen, Leena Mannonen

Research output: Contribution to journalArticleScientificpeer-review

31 Citations (Scopus)

Abstract

In recent years particle bombardment has become the most used method for gene transfer to barley (Hordeum vulgare L.). Transformation efficiency depends greatly on the ability of the target material to regenerate into green plants. In this work we improved the regeneration efficiency of polyembryogenic cultures of barley (cv. Kymppi) by optimizing the nitrogen and copper concentrations in the media.
Original languageEnglish
Pages (from-to)85 - 92
Number of pages8
JournalPlant Science
Volume151
Issue number1
DOIs
Publication statusPublished - 2000
MoE publication typeA1 Journal article-refereed

Fingerprint

Viridiplantae
Hordeum
Hordeum vulgare
Copper
Regeneration
Nitrogen
copper
barley
nitrogen
gene transfer
Genes
methodology

Keywords

  • Barley
  • Hordeum vulgare
  • Mathematical models
  • Medium optimization
  • Polyembryogenesis
  • Regeneration

Cite this

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abstract = "In recent years particle bombardment has become the most used method for gene transfer to barley (Hordeum vulgare L.). Transformation efficiency depends greatly on the ability of the target material to regenerate into green plants. In this work we improved the regeneration efficiency of polyembryogenic cultures of barley (cv. Kymppi) by optimizing the nitrogen and copper concentrations in the media.",
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pages = "85 -- 92",
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Optimization of media nitrogen and copper concentrations for regeneration of green plants from polyembryogenic cultures of barley (Hordeum vulgare L.). / Nuutila, Anna-Maria (Corresponding Author); Hämäläinen, Jari; Mannonen, Leena.

In: Plant Science, Vol. 151, No. 1, 2000, p. 85 - 92.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Optimization of media nitrogen and copper concentrations for regeneration of green plants from polyembryogenic cultures of barley (Hordeum vulgare L.)

AU - Nuutila, Anna-Maria

AU - Hämäläinen, Jari

AU - Mannonen, Leena

PY - 2000

Y1 - 2000

N2 - In recent years particle bombardment has become the most used method for gene transfer to barley (Hordeum vulgare L.). Transformation efficiency depends greatly on the ability of the target material to regenerate into green plants. In this work we improved the regeneration efficiency of polyembryogenic cultures of barley (cv. Kymppi) by optimizing the nitrogen and copper concentrations in the media.

AB - In recent years particle bombardment has become the most used method for gene transfer to barley (Hordeum vulgare L.). Transformation efficiency depends greatly on the ability of the target material to regenerate into green plants. In this work we improved the regeneration efficiency of polyembryogenic cultures of barley (cv. Kymppi) by optimizing the nitrogen and copper concentrations in the media.

KW - Barley

KW - Hordeum vulgare

KW - Mathematical models

KW - Medium optimization

KW - Polyembryogenesis

KW - Regeneration

U2 - 10.1016/S0168-9452(99)00202-2

DO - 10.1016/S0168-9452(99)00202-2

M3 - Article

VL - 151

SP - 85

EP - 92

JO - Plant Science

JF - Plant Science

SN - 0168-9452

IS - 1

ER -