Optimization of media nitrogen
 and copper concentrations for regeneration of green plants from 
polyembryogenic cultures of barley (Hordeum vulgare L.)

Anna-Maria Nuutila; Jari Hämäläinen; Leena Mannonen

doi:10.1016/S0168-9452(99)00202-2

Optimization of media nitrogen and copper concentrations for regeneration of green plants from polyembryogenic cultures of barley (Hordeum vulgare L.)

Anna-Maria Nuutila (Corresponding Author), Jari Hämäläinen, Leena Mannonen

Research output: Contribution to journal › Article › Scientific › peer-review

38 Citations (Scopus)

Abstract

In recent years particle bombardment has become the most used method for gene transfer to barley (Hordeum vulgare L.). Transformation efficiency depends greatly on the ability of the target material to regenerate into green plants. In this work we improved the regeneration efficiency of polyembryogenic cultures of barley (cv. Kymppi) by optimizing the nitrogen and copper concentrations in the media.

Original language	English
Pages (from-to)	85 - 92
Number of pages	8
Journal	Plant Science
Volume	151
Issue number	1
DOIs	https://doi.org/10.1016/S0168-9452(99)00202-2
Publication status	Published - 2000
MoE publication type	A1 Journal article-refereed

Keywords

Barley
Hordeum vulgare
Mathematical models
Medium optimization
Polyembryogenesis
Regeneration

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10.1016/S0168-9452(99)00202-2

Cite this

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title = "Optimization of media nitrogen and copper concentrations for regeneration of green plants from polyembryogenic cultures of barley (Hordeum vulgare L.)",

abstract = "In recent years particle bombardment has become the most used method for gene transfer to barley (Hordeum vulgare L.). Transformation efficiency depends greatly on the ability of the target material to regenerate into green plants. In this work we improved the regeneration efficiency of polyembryogenic cultures of barley (cv. Kymppi) by optimizing the nitrogen and copper concentrations in the media.",

keywords = "Barley, Hordeum vulgare, Mathematical models, Medium optimization, Polyembryogenesis, Regeneration",

author = "Anna-Maria Nuutila and Jari H{\"a}m{\"a}l{\"a}inen and Leena Mannonen",

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language = "English",

volume = "151",

pages = "85 -- 92",

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Optimization of media nitrogen and copper concentrations for regeneration of green plants from polyembryogenic cultures of barley (Hordeum vulgare L.). / Nuutila, Anna-Maria (Corresponding Author); Hämäläinen, Jari; Mannonen, Leena.
In: Plant Science, Vol. 151, No. 1, 2000, p. 85 - 92.

Research output: Contribution to journal › Article › Scientific › peer-review

TY - JOUR

T1 - Optimization of media nitrogen and copper concentrations for regeneration of green plants from polyembryogenic cultures of barley (Hordeum vulgare L.)

AU - Nuutila, Anna-Maria

AU - Hämäläinen, Jari

AU - Mannonen, Leena

PY - 2000

Y1 - 2000

N2 - In recent years particle bombardment has become the most used method for gene transfer to barley (Hordeum vulgare L.). Transformation efficiency depends greatly on the ability of the target material to regenerate into green plants. In this work we improved the regeneration efficiency of polyembryogenic cultures of barley (cv. Kymppi) by optimizing the nitrogen and copper concentrations in the media.

AB - In recent years particle bombardment has become the most used method for gene transfer to barley (Hordeum vulgare L.). Transformation efficiency depends greatly on the ability of the target material to regenerate into green plants. In this work we improved the regeneration efficiency of polyembryogenic cultures of barley (cv. Kymppi) by optimizing the nitrogen and copper concentrations in the media.

KW - Barley

KW - Hordeum vulgare

KW - Mathematical models

KW - Medium optimization

KW - Polyembryogenesis

KW - Regeneration

U2 - 10.1016/S0168-9452(99)00202-2

DO - 10.1016/S0168-9452(99)00202-2

M3 - Article

SN - 0168-9452

VL - 151

SP - 85

EP - 92

JO - Plant Science

JF - Plant Science

IS - 1

ER -

Optimization of media nitrogen and copper concentrations for regeneration of green plants from polyembryogenic cultures of barley (Hordeum vulgare L.)

Abstract

Keywords

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