Optimization of media nitrogen and copper concentrations for regeneration of green plants from polyembryogenic cultures of barley (Hordeum vulgare L.)

Anna-Maria Nuutila (Corresponding Author), Jari Hämäläinen, Leena Mannonen

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    32 Citations (Scopus)


    In recent years particle bombardment has become the most used method for gene transfer to barley (Hordeum vulgare L.). Transformation efficiency depends greatly on the ability of the target material to regenerate into green plants. In this work we improved the regeneration efficiency of polyembryogenic cultures of barley (cv. Kymppi) by optimizing the nitrogen and copper concentrations in the media.
    Original languageEnglish
    Pages (from-to)85 - 92
    Number of pages8
    JournalPlant Science
    Issue number1
    Publication statusPublished - 2000
    MoE publication typeA1 Journal article-refereed



    • Barley
    • Hordeum vulgare
    • Mathematical models
    • Medium optimization
    • Polyembryogenesis
    • Regeneration

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