Optimization of media nitrogen and copper concentrations for regeneration of green plants from polyembryogenic cultures of barley (Hordeum vulgare L.)

Anna-Maria Nuutila (Corresponding Author), Jari Hämäläinen, Leena Mannonen

    Research output: Contribution to journalArticleScientificpeer-review

    32 Citations (Scopus)

    Abstract

    In recent years particle bombardment has become the most used method for gene transfer to barley (Hordeum vulgare L.). Transformation efficiency depends greatly on the ability of the target material to regenerate into green plants. In this work we improved the regeneration efficiency of polyembryogenic cultures of barley (cv. Kymppi) by optimizing the nitrogen and copper concentrations in the media.
    Original languageEnglish
    Pages (from-to)85 - 92
    Number of pages8
    JournalPlant Science
    Volume151
    Issue number1
    DOIs
    Publication statusPublished - 2000
    MoE publication typeA1 Journal article-refereed

    Fingerprint

    Viridiplantae
    Hordeum
    Hordeum vulgare
    Copper
    Regeneration
    Nitrogen
    copper
    barley
    nitrogen
    gene transfer
    Genes
    methodology

    Keywords

    • Barley
    • Hordeum vulgare
    • Mathematical models
    • Medium optimization
    • Polyembryogenesis
    • Regeneration

    Cite this

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    title = "Optimization of media nitrogen and copper concentrations for regeneration of green plants from polyembryogenic cultures of barley (Hordeum vulgare L.)",
    abstract = "In recent years particle bombardment has become the most used method for gene transfer to barley (Hordeum vulgare L.). Transformation efficiency depends greatly on the ability of the target material to regenerate into green plants. In this work we improved the regeneration efficiency of polyembryogenic cultures of barley (cv. Kymppi) by optimizing the nitrogen and copper concentrations in the media.",
    keywords = "Barley, Hordeum vulgare, Mathematical models, Medium optimization, Polyembryogenesis, Regeneration",
    author = "Anna-Maria Nuutila and Jari H{\"a}m{\"a}l{\"a}inen and Leena Mannonen",
    year = "2000",
    doi = "10.1016/S0168-9452(99)00202-2",
    language = "English",
    volume = "151",
    pages = "85 -- 92",
    journal = "Plant Science",
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    number = "1",

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    Optimization of media nitrogen and copper concentrations for regeneration of green plants from polyembryogenic cultures of barley (Hordeum vulgare L.). / Nuutila, Anna-Maria (Corresponding Author); Hämäläinen, Jari; Mannonen, Leena.

    In: Plant Science, Vol. 151, No. 1, 2000, p. 85 - 92.

    Research output: Contribution to journalArticleScientificpeer-review

    TY - JOUR

    T1 - Optimization of media nitrogen and copper concentrations for regeneration of green plants from polyembryogenic cultures of barley (Hordeum vulgare L.)

    AU - Nuutila, Anna-Maria

    AU - Hämäläinen, Jari

    AU - Mannonen, Leena

    PY - 2000

    Y1 - 2000

    N2 - In recent years particle bombardment has become the most used method for gene transfer to barley (Hordeum vulgare L.). Transformation efficiency depends greatly on the ability of the target material to regenerate into green plants. In this work we improved the regeneration efficiency of polyembryogenic cultures of barley (cv. Kymppi) by optimizing the nitrogen and copper concentrations in the media.

    AB - In recent years particle bombardment has become the most used method for gene transfer to barley (Hordeum vulgare L.). Transformation efficiency depends greatly on the ability of the target material to regenerate into green plants. In this work we improved the regeneration efficiency of polyembryogenic cultures of barley (cv. Kymppi) by optimizing the nitrogen and copper concentrations in the media.

    KW - Barley

    KW - Hordeum vulgare

    KW - Mathematical models

    KW - Medium optimization

    KW - Polyembryogenesis

    KW - Regeneration

    U2 - 10.1016/S0168-9452(99)00202-2

    DO - 10.1016/S0168-9452(99)00202-2

    M3 - Article

    VL - 151

    SP - 85

    EP - 92

    JO - Plant Science

    JF - Plant Science

    SN - 0168-9452

    IS - 1

    ER -